LOCUS       sequence-425332-       10709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of mVenus flanked with an 
            N-terminal 50 amino acids of chloroplas outer envelope membrane 
            transit peptide in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Ichikawa S, Ishikawa K, Miyakawa H, Kodama Y
  TITLE     Live-cell imaging of the chloroplast outer envelope membrane using 
            fluorescent dyes.
  JOURNAL   Plant Direct. 2022 Nov 15;6(11):e462. doi: 10.1002/pld3.462. 
            eCollection 2022 Nov.
  PUBMED    36398034
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2022-11-15"; volume: "6(11):e462. doi"; pages: " 
            10.1002/pld3.462. eCollection 2022 Nov"
COMMENT     Sequence Label: pGWB602-OEP7-mVenus
FEATURES             Location/Qualifiers
     source          1..10709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        658..1003
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     946..966
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1098..1122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1311..1313,1314..1316,1317..2030)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1356..1377)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1617..1636)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1964..1985
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2040..2064)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2096..2348
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2381..2397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2381..2397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2394..2416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2405..2421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2429..2435,2436..2453,2454..2459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2474..2495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2531..2783)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2943..3494)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(3546..3725)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4231..4255
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4776..5567
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5813..6401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6302..6321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6555..6572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6587..6727
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6653..6672)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6813..6835
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7071..7265
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7331..8404)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8833..9462)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgccaag gaaaggctat cgttcaagat
      481 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
      541 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
      601 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
      661 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
      721 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
      781 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      841 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      901 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
      961 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtg
     1021 tatttttaca acaattacca acaacaacaa acaacaaaca acattacaat tactatttac
     1081 aatttctaga gttatcaaca agtttgtaca aaaaagcagg ctttaaagga accaattcag
     1141 tcgacatggg aaaaacttcg ggagcgaaac aggcgactgt ggtggtcgca gcgatggcgt
     1201 taggatggtt agccatagag atcgctttca agcctttcct cgataaattc cgctcctcaa
     1261 tcgacaaatc tgacccaacc aaagaccccg atgacggcgg tagcggcggt atggtgagca
     1321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1381 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1441 ccctgaagct catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1501 ccctcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     1561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1741 acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     1801 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     1861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1921 accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1981 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gatatctaga
     2041 cccagctttc ttgtacaaag tggttgataa cagcgcttag agctcgaatt tccccgatcg
     2101 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     2161 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     2221 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     2281 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     2341 actagatcgg gaattggttc cggaaccaat tcgtaatcat gtcatagctg tttcctgtgt
     2401 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2461 cctggggtgc ctaatgagtg agctaactca cattaggctg aattaggcgc gcctatttct
     2521 attaattccc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
     2581 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     2641 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     2701 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     2761 tattgccaaa tgtttgaacg atcggggaaa ttcgagctgg ggacgtcccc agctcggtac
     2821 cccctcctgg gcacggtcgt acgcgatccg ccgcggttcc acctcaccat tgcgggtatg
     2881 ccagttgagt tcgatgagtt cccgtaccag ctccaactcg ggcgatgcca cgcgcactcc
     2941 gttcagatct cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc
     3001 tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg
     3061 cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg
     3121 acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc
     3181 gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc
     3241 cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg
     3301 tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac
     3361 tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg
     3421 acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt
     3481 cgttctgggc tcatatctta ttgccccccg ggatcagatc tggattgaga gtgaatatga
     3541 gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa
     3601 atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac
     3661 gtatgtgctt agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc
     3721 ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg
     3781 tgactccctt aattctccgc tcatgatctt gatcccctgc gccatcagat ccttggcggc
     3841 aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct
     3901 ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc
     3961 ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag
     4021 tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc
     4081 ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgaagctga tccgtcgaga
     4141 ttaatagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa acgtccgcaa
     4201 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     4261 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     4321 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     4381 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     4441 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
     4501 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     4561 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     4621 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     4681 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     4741 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag
     4801 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     4861 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     4921 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     4981 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     5041 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     5101 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     5161 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     5221 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     5281 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     5341 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     5401 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     5461 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     5521 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa
     5581 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag
     5641 cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg
     5701 cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg
     5761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5941 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6481 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6541 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     6601 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     6661 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     6721 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6781 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6841 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     6901 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     6961 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     7021 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     7081 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     7141 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     7201 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     7261 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     7321 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     7381 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     7441 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     7501 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     7561 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     7621 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     7681 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     7741 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     7801 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
     7861 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
     7921 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
     7981 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
     8041 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
     8101 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
     8161 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
     8221 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
     8281 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
     8341 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
     8401 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca
     8461 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
     8521 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
     8581 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
     8641 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
     8701 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
     8761 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
     8821 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
     8881 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
     8941 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
     9001 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
     9061 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
     9121 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
     9181 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
     9241 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
     9301 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
     9361 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
     9421 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
     9481 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
     9541 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
     9601 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
     9661 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
     9721 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
     9781 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
     9841 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
     9901 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
     9961 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    10021 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    10081 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    10141 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    10201 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    10261 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    10321 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    10381 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    10441 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    10501 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    10561 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    10621 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    10681 cacatacaaa tggacgaacg gataaacct
//
