LOCUS       sequence-425339-       13297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of GBBSI fused to the tagRFP in 
            plants (for Agrobacterium-mediated genetic transformation.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13297)
  AUTHORS   Ichikawa S, Sakata M, Oba T, Kodama Y
  TITLE     Fluorescein staining of chloroplast starch granules in living 
            plants.
  JOURNAL   Plant Physiol. 2023 Oct 4:kiad528. doi: 10.1093/plphys/kiad528.
  PUBMED    37792703
REFERENCE   2  (bases 1 to 13297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Oct 4:kiad528. doi: 10.1093/plphys/kiad528."
COMMENT     Sequence Label: pGWB560-GBSSI
FEATURES             Location/Qualifiers
     source          1..13297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4159..4183)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4194..4907
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             complement(5057..6082)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6134..6313)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6819..6843
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7364..8155
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8401..8989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8890..8909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9143..9160
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9175..9315
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9241..9260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9401..9423
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9659..9853
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9919..10992)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11421..12050)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctttaaagg aaccaattca gtcgacatgg caactgtgac tgcttcttct aactttgtgt
     1261 caagaacttc acttttcaac aatcatggtg cttcttcatg ctctgatgtc gctcagatta
     1321 ccttaaaagg ccaatccttg actcattgtg ggttaaggtc attcaacatg gtggataacc
     1381 ttcagaggag atctcaagct aaacctgttt ctgctaaatc ctcaaagaga tcttctaaag
     1441 ttaagactgc tggtaagatt gtgtgtgaga aaggaatgtc tgtgattttt attggagctg
     1501 aagttggtcc atggagtaaa actggtggtc ttggtgatgt tctcggtggt ctacctccag
     1561 ctcttgctgt aagttcatat aaacattagc ttgatttgag gtctttactt gttttttgat
     1621 gaacctacat gttatgactt ttgttaggct agaggccacc gtgtgatgac aatttgtcct
     1681 cggtatgacc aatataaaga tgcttgggac acttgtgttg tggttcaggt ataattctct
     1741 caagtttctt ttgctctgta tggaccttga tgaattttgg atgcttaggt aagtcatctt
     1801 ggaatgttat ttgatgcaga tcaaagttgg ggataaagtt gagaatgttc gtttcttcca
     1861 ttgctacaaa cgaggagttg atcgtgtctt tgttgaccat ccaatctttc ttgctaaggt
     1921 ctgtttatat tggttagata atcacaaggg tgtattgtac tttgagctaa tgaggaatgg
     1981 taataatgca ggttgtgggc aaaacaggat ccaaaatcta tggtcctata actggagtag
     2041 actacaatga caaccaactc cggttcagtt tgttgtgtca ggtaacactc tttcctctta
     2101 cttctgtagt tacagttgtg ttgtttctga atcgtgggtt ctcatttgat tgaaacatgt
     2161 agcctacaga gattaaattc ataaataatg cagaatctgt gtttgaatag cgtacaagca
     2221 tgagcaataa cttgatttac tttgatcgtt ggtaggctgc tcttgaggca ccacaggttc
     2281 tgaacctgaa cagcagcaag tacttctctg gaccatatgg taagaaaatg atttctcaat
     2341 tcttaattaa aatggtttct aaaaaataaa acttcaattt ttgaaaagta acaaagccta
     2401 tctctctgat gttataggtg aagatgtagt ctttgttgcc aatgactggc acactgctct
     2461 acttccatgt tacctcaaat ctatgtatca atcccgcgga gtctacatga atgcaaaggt
     2521 atagatataa tttttgttcc ttcatcatgt taattagcta ttcatcttaa agtagcaaac
     2581 attaccaaat cttgaactga aactctagct ttgatacttt ttacaatgaa tatcaaaata
     2641 ttttactttt ttttaggtgg tcttctgcat tcacaacata gcctaccagg gaagatttgc
     2701 ctttgatgac tattcccttc tcaacttgcc catcagcttt aaaagttctt tcgacttcat
     2761 ggacgggtac tggtttctct gaagaacaga tcattacacc attttgtctc atgtttcaat
     2821 cttaatttac tgtaacttct tgttccattt tcaggtatga aaagccagta aaaggacgga
     2881 aaattaactg gatgaaggct gcaattctgg aagctcaccg tgtcttaaca gttagtccat
     2941 actacgctca agaactcatc tctggagttg atagaggcgt ggaattgcat aaatatcttc
     3001 gaatgaaaac agtttccgga attattaatg gaatggatgt tcaagaatgg aacccgtcta
     3061 ctgacaagta catcgatatc aaatacgata ttaccactgt aagatttctc ttgaagatca
     3121 gtgcattagg acagaagaag ttgcgggaaa tctttaccga tcttgctact tgtttttaca
     3181 ggttacagat gctaaaccat tgatcaaaga agcacttcag gctgctgttg gacttcccgt
     3241 ggacagggat gtcccggtta tcggtttcat agggagattg gaggagcaga agggttctga
     3301 tattctagtg gaagctattt ccaagttcat ggggctcaat gttcagatgg ttatccttgt
     3361 gagtacacta agcagtgaac ttgtgatcat aacgtcttta ggatcacaaa gttattgatt
     3421 cttgtctgat gtttttcaca ggggactgga aagaagaaga tggaggctca gattcttgaa
     3481 ctagaagaga agttcccagg gaaggcggtt ggagtggcga aattcaacgt gccattggct
     3541 catatgatca ctgctggagc tgacttcatc attgtcccaa gcaggtttga gccgtgtggt
     3601 ctcattcagc tgcacgcaat gagatatgga accgtcccta ttgtggcatc tactggtgga
     3661 cttgtggaca ctgtgaaaga tggctacaca ggtttccaca ttggaagatt caacgtcaag
     3721 gtaactcaaa atcaccagct tttcgcgctt tttctacaac agtttctttc gacatgacaa
     3781 caaattgatc taaaatgagt taccattttg ctttgttata tagtgtgaag ttgtggatcc
     3841 agatgatgtg atagcaacag caaaggctgt gacaagagcc gttgcagtat atggaacatc
     3901 cgcaatgcaa gaaatggtca agaactgcat ggaccaagac ttctcctgga aggtcagtga
     3961 ttcagttcat tctaaagttt gaaactttgt ttatattgag ttgttgacat caatcttgat
     4021 ttgatgttat gcagggacct gcgaggttgt gggagaaggt actattgtcc cttaatgtgg
     4081 cgggaagtga agccggaacc gagggtgaag agatagctcc tctggccaag gagaacgtag
     4141 cgacgccggg atatctagac ccagctttct tgtacaaagt ggttgataac agcatggtgt
     4201 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     4261 tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     4321 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     4381 ctaccagctt catgtacggc agcagaacct tcatcaacca cacccagggc atccccgact
     4441 tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     4501 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     4561 tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
     4621 gctgggaggc caacaccgag atgctgtacc ccgctgacgg cggcctggaa ggcagaagcg
     4681 acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttcaag accacataca
     4741 gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg gaccacagac
     4801 tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg
     4861 ccagatactg cgacctccct agcaaactgg ggcacaaact taattgagct tagagctggg
     4921 gatcgatccc gcgaactgtg gacgagaact gtgccaccaa gcgtaaggcc gttctctcgc
     4981 atttgccttg ctaggctcgc gcgagttgct ggctgaggcg ttctcgaaat cagctcttgt
     5041 tcggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac
     5101 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
     5161 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
     5221 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
     5281 ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt
     5341 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
     5401 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
     5461 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
     5521 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
     5581 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
     5641 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
     5701 taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca
     5761 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     5821 tcaggctctc gctgaatgcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     5881 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     5941 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     6001 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     6061 cggtgagttc aggctttttc atatcttatt gccccccggg atcagatctg gattgagagt
     6121 gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt
     6181 gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg
     6241 tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc
     6301 aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg
     6361 tcataacgtg actcccttaa ttctccgctc atgatcttga tcccctgcgc catcagatcc
     6421 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     6481 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     6541 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     6601 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     6661 tgttccgctt cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gaagctgatc
     6721 cgtcgagatt aatagaaata aattcagcct aattcggcgt taattcagta cattaaaaac
     6781 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     6841 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     6901 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     6961 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     7021 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     7081 aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     7141 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     7201 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     7261 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     7321 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat
     7381 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     7441 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     7501 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     7561 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     7621 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     7681 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     7741 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     7801 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     7861 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     7921 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     7981 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     8041 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     8101 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     8161 agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     8221 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     8281 taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc
     8341 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8401 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8461 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8521 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     8581 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8641 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8701 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8761 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8821 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8881 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8941 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9001 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9061 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9121 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     9181 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     9241 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     9301 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     9361 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     9421 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     9481 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     9541 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     9601 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     9661 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     9721 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     9781 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     9841 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     9901 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     9961 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
    10021 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
    10081 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
    10141 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
    10201 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
    10261 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
    10321 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
    10381 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
    10441 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
    10501 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
    10561 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
    10621 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
    10681 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
    10741 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
    10801 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
    10861 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
    10921 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
    10981 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
    11041 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
    11101 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
    11161 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
    11221 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
    11281 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
    11341 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
    11401 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
    11461 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
    11521 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
    11581 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
    11641 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
    11701 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
    11761 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
    11821 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
    11881 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
    11941 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
    12001 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
    12061 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
    12121 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
    12181 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
    12241 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
    12301 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
    12361 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
    12421 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
    12481 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
    12541 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
    12601 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
    12661 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
    12721 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
    12781 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
    12841 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
    12901 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
    12961 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
    13021 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
    13081 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
    13141 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
    13201 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
    13261 ttggcatgca catacaaatg gacgaacgga taaacct
//
