LOCUS       sequence-425341-       13557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of GBBSI fused to the sGFP in 
            plants (for Agrobacterium-mediated genetic transformation.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13557)
  AUTHORS   Ichikawa S, Sakata M, Oba T, Kodama Y
  TITLE     Fluorescein staining of chloroplast starch granules in living 
            plants.
  JOURNAL   Plant Physiol. 2023 Oct 4:kiad528. doi: 10.1093/plphys/kiad528.
  PUBMED    37792703
REFERENCE   2  (bases 1 to 13557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Oct 4:kiad528. doi: 10.1093/plphys/kiad528."
COMMENT     Sequence Label: pGWB605-GBSSI
FEATURES             Location/Qualifiers
     source          1..13557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4159..4183)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4200..4202,4203..4205,4206..4919)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4245..4266)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4506..4525)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4853..4874
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4944..5196
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5229..5245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5229..5245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5242..5264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5253..5269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5277..5283,5284..5301,5302..5307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5322..5343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5379..5631)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5791..6342)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(6394..6573)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7079..7103
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7624..8415
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8661..9249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9150..9169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9403..9420
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9435..9575
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9501..9520)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9661..9683
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9919..10113
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10179..11252)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11681..12310)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctttaaagg aaccaattca gtcgacatgg caactgtgac tgcttcttct aactttgtgt
     1261 caagaacttc acttttcaac aatcatggtg cttcttcatg ctctgatgtc gctcagatta
     1321 ccttaaaagg ccaatccttg actcattgtg ggttaaggtc attcaacatg gtggataacc
     1381 ttcagaggag atctcaagct aaacctgttt ctgctaaatc ctcaaagaga tcttctaaag
     1441 ttaagactgc tggtaagatt gtgtgtgaga aaggaatgtc tgtgattttt attggagctg
     1501 aagttggtcc atggagtaaa actggtggtc ttggtgatgt tctcggtggt ctacctccag
     1561 ctcttgctgt aagttcatat aaacattagc ttgatttgag gtctttactt gttttttgat
     1621 gaacctacat gttatgactt ttgttaggct agaggccacc gtgtgatgac aatttgtcct
     1681 cggtatgacc aatataaaga tgcttgggac acttgtgttg tggttcaggt ataattctct
     1741 caagtttctt ttgctctgta tggaccttga tgaattttgg atgcttaggt aagtcatctt
     1801 ggaatgttat ttgatgcaga tcaaagttgg ggataaagtt gagaatgttc gtttcttcca
     1861 ttgctacaaa cgaggagttg atcgtgtctt tgttgaccat ccaatctttc ttgctaaggt
     1921 ctgtttatat tggttagata atcacaaggg tgtattgtac tttgagctaa tgaggaatgg
     1981 taataatgca ggttgtgggc aaaacaggat ccaaaatcta tggtcctata actggagtag
     2041 actacaatga caaccaactc cggttcagtt tgttgtgtca ggtaacactc tttcctctta
     2101 cttctgtagt tacagttgtg ttgtttctga atcgtgggtt ctcatttgat tgaaacatgt
     2161 agcctacaga gattaaattc ataaataatg cagaatctgt gtttgaatag cgtacaagca
     2221 tgagcaataa cttgatttac tttgatcgtt ggtaggctgc tcttgaggca ccacaggttc
     2281 tgaacctgaa cagcagcaag tacttctctg gaccatatgg taagaaaatg atttctcaat
     2341 tcttaattaa aatggtttct aaaaaataaa acttcaattt ttgaaaagta acaaagccta
     2401 tctctctgat gttataggtg aagatgtagt ctttgttgcc aatgactggc acactgctct
     2461 acttccatgt tacctcaaat ctatgtatca atcccgcgga gtctacatga atgcaaaggt
     2521 atagatataa tttttgttcc ttcatcatgt taattagcta ttcatcttaa agtagcaaac
     2581 attaccaaat cttgaactga aactctagct ttgatacttt ttacaatgaa tatcaaaata
     2641 ttttactttt ttttaggtgg tcttctgcat tcacaacata gcctaccagg gaagatttgc
     2701 ctttgatgac tattcccttc tcaacttgcc catcagcttt aaaagttctt tcgacttcat
     2761 ggacgggtac tggtttctct gaagaacaga tcattacacc attttgtctc atgtttcaat
     2821 cttaatttac tgtaacttct tgttccattt tcaggtatga aaagccagta aaaggacgga
     2881 aaattaactg gatgaaggct gcaattctgg aagctcaccg tgtcttaaca gttagtccat
     2941 actacgctca agaactcatc tctggagttg atagaggcgt ggaattgcat aaatatcttc
     3001 gaatgaaaac agtttccgga attattaatg gaatggatgt tcaagaatgg aacccgtcta
     3061 ctgacaagta catcgatatc aaatacgata ttaccactgt aagatttctc ttgaagatca
     3121 gtgcattagg acagaagaag ttgcgggaaa tctttaccga tcttgctact tgtttttaca
     3181 ggttacagat gctaaaccat tgatcaaaga agcacttcag gctgctgttg gacttcccgt
     3241 ggacagggat gtcccggtta tcggtttcat agggagattg gaggagcaga agggttctga
     3301 tattctagtg gaagctattt ccaagttcat ggggctcaat gttcagatgg ttatccttgt
     3361 gagtacacta agcagtgaac ttgtgatcat aacgtcttta ggatcacaaa gttattgatt
     3421 cttgtctgat gtttttcaca ggggactgga aagaagaaga tggaggctca gattcttgaa
     3481 ctagaagaga agttcccagg gaaggcggtt ggagtggcga aattcaacgt gccattggct
     3541 catatgatca ctgctggagc tgacttcatc attgtcccaa gcaggtttga gccgtgtggt
     3601 ctcattcagc tgcacgcaat gagatatgga accgtcccta ttgtggcatc tactggtgga
     3661 cttgtggaca ctgtgaaaga tggctacaca ggtttccaca ttggaagatt caacgtcaag
     3721 gtaactcaaa atcaccagct tttcgcgctt tttctacaac agtttctttc gacatgacaa
     3781 caaattgatc taaaatgagt taccattttg ctttgttata tagtgtgaag ttgtggatcc
     3841 agatgatgtg atagcaacag caaaggctgt gacaagagcc gttgcagtat atggaacatc
     3901 cgcaatgcaa gaaatggtca agaactgcat ggaccaagac ttctcctgga aggtcagtga
     3961 ttcagttcat tctaaagttt gaaactttgt ttatattgag ttgttgacat caatcttgat
     4021 ttgatgttat gcagggacct gcgaggttgt gggagaaggt actattgtcc cttaatgtgg
     4081 cgggaagtga agccggaacc gagggtgaag agatagctcc tctggccaag gagaacgtag
     4141 cgacgccggg atatctagac ccagctttct tgtacaaagt ggttcgatct agaggatcca
     4201 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4261 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4321 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4381 tcgtgaccac cttcacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4441 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4501 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4561 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4621 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4681 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4741 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4801 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4861 tgctggagtt cgtgaccgcc gccgggatca ctcacggcat ggacgagctg tacaagtaaa
     4921 gcggcccgag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     4981 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     5041 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     5101 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     5161 aattatcgcg cgcggtgtca tctatgttac tagatcggga attggttccg gaaccaattc
     5221 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5281 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5341 ttaggctgaa ttaggcgcgc ctatttctat taattcccga tctagtaaca tagatgacac
     5401 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat
     5461 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat
     5521 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc
     5581 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt
     5641 cgagctgggg acgtccccag ctcggtaccc cctcctgggc acggtcgtac gcgatccgcc
     5701 gcggttccac ctcaccattg cgggtatgcc agttgagttc gatgagttcc cgtaccagct
     5761 ccaactcggg cgatgccacg cgcactccgt tcagatctcg gtgacgggca ggaccggacg
     5821 gggcggtacc ggcaggctga agtccagctg ccagaaaccc acgtcatgcc agttcccgtg
     5881 cttgaagccg gccgcccgca gcatgccgcg gggggcatat ccgagcgcct cgtgcatgcg
     5941 cacgctcggg tcgttgggca gcccgatgac agcgaccacg ctcttgaagc cctgtgcctc
     6001 cagggacttc agcaggtggg tgtagagcgt ggagcccagt cccgtccgct ggtggcgggg
     6061 ggagacgtac acggtcgact cggccgtcca gtcgtaggcg ttgcgtgcct tccaggggcc
     6121 cgcgtaggcg atgccggcga cctcgccgtc cacctcggcg acgagccagg gatagcgctc
     6181 ccgcagacgg acgaggtcgt ccgtccactc ctgcggttcc tgcggctcgg tacggaagtt
     6241 gaccgtgctt gtctcgatgt agtggttgac gatggtgcag accgccggca tgtccgcctc
     6301 ggtggcacgg cggatgtcgg ccgggcgtcg ttctgggctc atatcttatt gccccccggg
     6361 atcagatctg gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg
     6421 aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac
     6481 ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc
     6541 cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg
     6601 tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcttga
     6661 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     6721 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac
     6781 cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct
     6841 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     6901 ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgagtgctt
     6961 gcggcagcgt gaagctgatc cgtcgagatt aatagaaata aattcagcct aattcggcgt
     7021 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     7081 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
     7141 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
     7201 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
     7261 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
     7321 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt
     7381 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     7441 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     7501 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     7561 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     7621 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     7681 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     7741 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     7801 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     7861 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     7921 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     7981 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     8041 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     8101 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     8161 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     8221 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     8281 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     8341 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     8401 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg
     8461 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     8521 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     8581 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9361 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9421 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     9481 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     9541 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     9601 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     9661 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
     9721 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
     9781 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
     9841 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
     9901 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
     9961 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
    10021 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
    10081 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
    10141 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
    10201 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
    10261 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
    10321 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
    10381 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
    10441 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
    10501 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
    10561 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
    10621 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
    10681 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
    10741 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
    10801 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
    10861 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
    10921 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
    10981 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
    11041 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
    11101 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
    11161 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
    11221 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
    11281 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc
    11341 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
    11401 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    11461 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    11521 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    11581 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    11641 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    11701 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    11761 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    11821 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    11881 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    11941 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    12001 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    12061 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    12121 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    12181 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    12241 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    12301 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    12361 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    12421 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    12481 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    12541 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    12601 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    12661 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    12721 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    12781 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    12841 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    12901 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    12961 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    13021 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    13081 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    13141 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    13201 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    13261 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    13321 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    13381 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    13441 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    13501 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
