LOCUS       sequence-425343-       11580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of sGFP flanked with an N-terminal 
            chloroplas inner envelope membrane transit peptide in plants ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Ichikawa S, Ishikawa K, Miyakawa H, Kodama Y
  TITLE     Live-cell imaging of the chloroplast outer envelope membrane using 
            fluorescent dyes.
  JOURNAL   Plant Direct. 2022 Nov 15;6(11):e462. doi: 10.1002/pld3.462. 
            eCollection 2022 Nov.
  PUBMED    36398034
REFERENCE   2  (bases 1 to 11580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2022-11-15"; volume: "6(11):e462. doi"; pages: " 
            10.1002/pld3.462. eCollection 2022 Nov"
COMMENT     Sequence Label: pGWB405-TIC21-sGFP
FEATURES             Location/Qualifiers
     source          1..11580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2734..2758)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2775..2777,2778..2780,2781..3494)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2820..2841)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3081..3100)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3428..3449
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(3898..4692)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4009..4028)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4619..4638
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4713..4896)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4749..4928)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5102..5126
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5647..6438
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6684..7272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7173..7192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7426..7443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7458..7598
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7524..7543)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7684..7706
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7942..8136
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8202..9275)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9704..10333)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctttaaagg aaccaattca gtcgacatgc aatcactact cttgccgccg gcgagttcct
     1261 ccggcgtatc cgccgtcgcg ctacggccag gtttccagca ctcatttaac caccaaagcc
     1321 tctctactcg ctccctgcca ttgtttaatc cacttctctt agctccgaag aagaaaacca
     1381 ccatctcctc atatcaatcg ccaccgtctc tgtccgtcta cggtttccaa atcggaggat
     1441 ctaaacctag tttcacacca tccacggtgg cattttcata tccgacttct ccgtcttccg
     1501 ttcccggtga taatgaggtc gacaaagcaa aactcgctca ggttctctat ccatttcttc
     1561 gcctttttca caagctagct gaaattgaat gtagaaagag agaaaatgat ctaacaaatc
     1621 gaaattagtt acaatctgct tttgatttta ggaagtagat aacaatttag gatttgagtt
     1681 ttcagaattg aaattttgaa aagtgcttag tgtgaagaat ctttatgcag taactgtatt
     1741 ggtgtggaag agattttttt tgttttatga gtatgggtgg tttattattc acaggttgca
     1801 aagagattgg agaagacttc aaggtacttt aagagactag ggagtattgg tttctggggg
     1861 cagcttgtgt caactgtggt tgcagctgtg attctatcct tctccattgt tgtaactggg
     1921 aaacctactt cacctgctac tttctatgct actgctagtg ggattgctgc tgcgtttgtc
     1981 tcggttttct ggtcgtttgg gtatattcgg ctctccgaga ggctccgtcg aacttccatc
     2041 gaccctgcca aggtaaatat gtcgttctct tcattgtagt tcaatcctaa tcagtgaaca
     2101 cccatacaat agcgcttgta ggtacgtatg gtcaactttg tttgttaaag accggtctgt
     2161 gaacctagat ttagtagcag ctcttgaacc ttggatcatg tccagtcagg ttgcccaggt
     2221 cacccaatag cggttactac tcctacttga gtcctgatac tcagttttgt agtttggtgt
     2281 ctaaaggaaa aaatccagtt ttaataaagg ttgtgttgtt gcaggcgccg cctcgcgctg
     2341 atgttgtgaa aggtttgagg agcgggatta tggtaaatat tttgggaatg ggatcagcac
     2401 tactcgggat gcaagcaaca gttggatttt tagttgcaaa ggctttaaca acttcagcaa
     2461 accctttcta ccaaggagtc tctcagggat acagtcccgt tcttgctctt gatgtcttcc
     2521 tagttcaggt attaagctct aattgtgttc ttattataag tcttacttta tttgtgtaag
     2581 ttcacttttt gacttgagag ccatttgtgc aggcatcggc gaacacctta ctctctcatt
     2641 tccttggtct tgtttgctcg ttggagctgt tgcggtctgt gacagtgccc aattcggaat
     2701 ctgtcgtagt tcctaaggtt gctggatatc tagacccagc tttcttgtac aaagtggttc
     2761 gatctagagg atccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2821 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2881 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2941 tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc agccgctacc
     3001 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3061 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3121 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3181 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     3241 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     3301 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3361 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     3421 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactcac ggcatggacg
     3481 agctgtacaa gtaaagcggc ccgagctcga atttcatccg ggtctgtggc gggaactcca
     3541 cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc
     3601 cgccgacgcc gttgatgtgg acgccgggcc cgatcatatt gtcgctcagg atcgtggcgt
     3661 tgtgcttgtc ggccgttgct gtcgtaatga tatcggcacc ttcgaccgcc tgttccgcag
     3721 agatcccgtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     3781 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     3841 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca
     3901 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     3961 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     4021 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     4081 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     4141 gagatcatcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     4201 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     4261 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     4321 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     4381 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     4441 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     4501 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     4561 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     4621 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     4681 ttgttcaatc atgcgaaacg atccagatcc ggtgcagatt atttggattg agagtgaata
     4741 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
     4801 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
     4861 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt
     4921 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
     4981 acgtgactcc cttaattctc cgctcatgat gaattcagaa ataaattcag cctaattcgg
     5041 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     5101 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     5161 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     5221 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     5281 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     5341 acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg
     5401 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     5461 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     5521 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     5581 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     5641 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     5701 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     5761 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     5821 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     5881 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     5941 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     6001 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     6061 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     6121 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     6181 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     6241 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     6301 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     6361 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     6421 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
     6481 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     6541 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     6601 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6661 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6721 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6781 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6841 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6901 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6961 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7021 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7081 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7141 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7201 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7261 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7321 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7381 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7441 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     7501 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     7561 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     7621 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7681 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     7741 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     7801 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     7861 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     7921 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     7981 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     8041 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     8101 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
     8161 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     8221 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     8281 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     8341 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     8401 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
     8461 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
     8521 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
     8581 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
     8641 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
     8701 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
     8761 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
     8821 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
     8881 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
     8941 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
     9001 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
     9061 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
     9121 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
     9181 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
     9241 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
     9301 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
     9361 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
     9421 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
     9481 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
     9541 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
     9601 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
     9661 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
     9721 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
     9781 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
     9841 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
     9901 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
     9961 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    10021 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    10081 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    10141 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    10201 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    10261 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    10321 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    10381 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    10441 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    10501 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    10561 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    10621 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    10681 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    10741 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    10801 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    10861 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    10921 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    10981 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    11041 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    11101 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    11161 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    11221 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    11281 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    11341 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    11401 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    11461 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    11521 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
