LOCUS       sequence-425344-       10818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of RBCS fused to the tagRFPn plants
            (for Agrobacterium-mediated genetic transformation.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Ichikawa S, Sakata M, Oba T, Kodama Y
  TITLE     Fluorescein staining of chloroplast starch granules in living 
            plants.
  JOURNAL   Plant Physiol. 2023 Oct 4:kiad528. doi: 10.1093/plphys/kiad528.
  PUBMED    37792703
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Oct 4:kiad528. doi: 10.1093/plphys/kiad528."
COMMENT     Sequence Label: pGWB602-RBCS-tagRFP
FEATURES             Location/Qualifiers
     source          1..10818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      16..268
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(301..317)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(301..317)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(314..336)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    325..341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(349..355,356..373,374..379))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    394..415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(451..703)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(863..1414)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1466..1645)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2151..2175
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2696..3487
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3733..4321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4222..4241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4475..4492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4507..4647
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4573..4592)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4733..4755
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4991..5185
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5251..6324)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6753..7382)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8683..8707
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8895..8917
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8909..8926
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8910..8926
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9034..9710
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9647..9667
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9799..9823
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10063..10776
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    complement(10778..10802)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
       61 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
      121 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
      181 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
      241 cgcgcggtgt catctatgtt actagatcgg gaattggttc cggaaccaat tcgtaatcat
      301 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      361 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaggctg
      421 aattaggcgc gcctatttct attaattccc gatctagtaa catagatgac accgcgcgcg
      481 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt
      541 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta
      601 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag
      661 gattcaatct taagaaactt tattgccaaa tgtttgaacg atcggggaaa ttcgagctgg
      721 ggacgtcccc agctcggtac cccctcctgg gcacggtcgt acgcgatccg ccgcggttcc
      781 acctcaccat tgcgggtatg ccagttgagt tcgatgagtt cccgtaccag ctccaactcg
      841 ggcgatgcca cgcgcactcc gttcagatct cggtgacggg caggaccgga cggggcggta
      901 ccggcaggct gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc
      961 cggccgcccg cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg
     1021 ggtcgttggg cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact
     1081 tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt
     1141 acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg
     1201 cgatgccggc gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac
     1261 ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc
     1321 ttgtctcgat gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac
     1381 ggcggatgtc ggccgggcgt cgttctgggc tcatatctta ttgccccccg ggatcagatc
     1441 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     1501 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     1561 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg
     1621 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     1681 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatctt gatcccctgc
     1741 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     1801 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     1861 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     1921 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     1981 tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc
     2041 gtgaagctga tccgtcgaga ttaatagaaa taaattcagc ctaattcggc gttaattcag
     2101 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     2161 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     2221 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     2281 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     2341 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     2401 ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     2461 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2521 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     2581 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     2641 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg
     2701 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     2761 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     2821 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     2881 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     2941 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3001 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3061 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3121 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3181 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3241 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3301 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3361 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3421 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     3481 caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     3541 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
     3601 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat
     3661 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3721 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3781 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3841 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3901 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3961 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4021 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4081 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4141 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4201 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4261 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4321 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4381 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4441 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4501 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     4561 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     4621 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     4681 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4741 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
     4801 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     4861 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     4921 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
     4981 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
     5041 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
     5101 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
     5161 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
     5221 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
     5281 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
     5341 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
     5401 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
     5461 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
     5521 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
     5581 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
     5641 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
     5701 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
     5761 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
     5821 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
     5881 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
     5941 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
     6001 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
     6061 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
     6121 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
     6181 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
     6241 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
     6301 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
     6361 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
     6421 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
     6481 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
     6541 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
     6601 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
     6661 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
     6721 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
     6781 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
     6841 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
     6901 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
     6961 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
     7021 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
     7081 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
     7141 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
     7201 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
     7261 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
     7321 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
     7381 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
     7441 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
     7501 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
     7561 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
     7621 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
     7681 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
     7741 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
     7801 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
     7861 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
     7921 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
     7981 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
     8041 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
     8101 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
     8161 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
     8221 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
     8281 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
     8341 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
     8401 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
     8461 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
     8521 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
     8581 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
     8641 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
     8701 cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa
     8761 gctgctctag cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     8821 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     8881 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcgta
     8941 ttggctagag cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat
     9001 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc
     9061 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga
     9121 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
     9181 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     9241 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg
     9301 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
     9361 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     9421 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
     9481 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc
     9541 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     9601 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     9661 acccttcctc tatataagga agttcatttc atttggagag gacacgctga aatcaccagt
     9721 gtatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa ttactattta
     9781 caatttctag agttatcaac aagtttgtac aaaaaagcag gcttcatggc ttcctctatg
     9841 ctctcttccg ctactatggt tgcctctccg gctcaggcca ctatggtcgc tcctttcaac
     9901 ggacttaagt cctccgctgc cttcccagcc acccgcaagg ctaacaacga cattacttcc
     9961 atcacaagca acggcggaag agttaactgc atgcaggtgt ggcctccgat tggaaagaag
    10021 aagtttgaga ctctctctta ccttcctgac cttaccgatt ccatggtgtc taagggcgaa
    10081 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac
    10141 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
    10201 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc
    10261 atgtacggca gcagaacctt catcaaccac acccagggca tccccgactt ctttaagcag
    10321 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
    10381 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
    10441 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
    10501 aacaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaaccga catggccctg
    10561 aagctcgtgg gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa
    10621 cccgctaaga acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc
    10681 aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc
    10741 gacctcccta gcaaactggg gcacaaactt aattgagacc cagctttctt gtacaaagtg
    10801 gttgataaca gcgcttag
//
