LOCUS       sequence-425353-        1985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for ceramide (putative, mammalian expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Girik V, van Ek L, Dentand Quadri I, Azam M, Cruz Cobo M, Mandavit 
            M, Riezman I, Riezman H, Gavin AC, Nunes-Hasler P
  TITLE     Development of Genetically Encoded Fluorescent KSR1-Based Probes to 
            Track Ceramides during Phagocytosis.
  JOURNAL   Int J Mol Sci. 2024 Mar 5;25(5):2996. doi: 10.3390/ijms25052996.
  PUBMED    38474242
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2024-03-5"; volume: "25(5):2996. doi"; pages: " 
            10.3390/ijms25052996"
COMMENT     Sequence Label: pEGFP-SET-EMD (N-SET-EMD)
FEATURES             Location/Qualifiers
     source          1..1985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        230..433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     384..404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      472..481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(478..480,481..483,484..1194)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(478..480,481..483,484..1194)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(523..544)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(784..803)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1131..1152
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1864..1985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1901..1920)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1955..1974
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
       61 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      121 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      181 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      241 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      301 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      361 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      421 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg
      481 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      541 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      601 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      661 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      721 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      781 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      841 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      901 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
      961 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1021 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1081 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1141 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga
     1201 ctcagatctc gagctggcgg ttcctctggt ggtggtggtg cgcagaagag gtcagaattg
     1261 atcgccaaaa tcccaaattt ttgggtaaca acatttgtca accatccaca agtgtctgca
     1321 ctgcttgggg aggaagatga agaggcactg cattatttga ccagagttga agtgacagaa
     1381 tttgaagata ttaaatcagg ttacagaata gatttttatt ttgatgaaaa tccttacttt
     1441 gaaaataaag ttctctccaa agaatttcat ctgaatgaga gtggtgatcc atcttcgaag
     1501 tccaccgaaa tcaaatggaa atctggaaag gatttgacga aacgttcgag tcaaacgcag
     1561 aataaagcca gcaggaagag gcagcatgag gaaccagaga gcttctttac ctggtttact
     1621 gaccattctg atgcaggtgc tgatgagtta ggagaggtca tcaaagatga tatttggcca
     1681 aacccattac agtactactt ggttcccgat atggattaag gtaccgcggg cccgggatcc
     1741 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     1801 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     1861 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1981 tctta
//
