LOCUS       sequence-425375-       12085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for Uni-Vector prime editing system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12085)
  AUTHORS   Huang CH, Chiu SY, Chou YC, Wu KJ
  TITLE     A refined Uni-vector prime editing system improves genome editing 
            outcomes in mammalian cells.
  JOURNAL   Biotechnol J. 2024 Feb;19(2):e2300353. doi: 10.1002/biot.202300353.
  PUBMED    38403398
REFERENCE   2  (bases 1 to 12085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J."; date: "2024-02"; volume: "19(2):e2300353. doi"; pages: " 
            10.1002/biot.202300353"
COMMENT     Sequence Label: pPE3-pPuro
FEATURES             Location/Qualifiers
     source          1..12085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..123
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        130..378
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     130..150
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     301..320
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2470..2681
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2689..2698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2701..2721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2725..6825
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSGFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8998..9018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9028..9084
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9085..9681
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9578..9598
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9688..10276
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9741..9761)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10159..10170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10283..10331
                     /note="synthetic polyadenylation signal"
     promoter        10353..10457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10458..10526,10527..11318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10676..10695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11489..12077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11978..11997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgagtttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
       61 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      121 tttactagtg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
      181 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
      241 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      301 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      361 tggaaaggac gaaacaccgg agacggtgtt gtaaatgagc acacaaaata cacatgctaa
      421 aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa taactttagt
      481 atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat gagcaaaata
      541 aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat agctactcca
      601 ccacctgttc ctaagtggtc agctcctgct tcaatcattt tttgagcatc ttcaaatgtt
      661 ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt cattagtcta
      721 acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac aaattctaat
      781 ccagcttcaa cagctatttc acaagctttc atgatttctt cttttgttaa taaacaattt
      841 tccataatac atttaacaac atgtgatcca gctgcttttt ttacagcttt catgtcttct
      901 aaaactaatt cataattttt gtcttttaat gcaccaatat ttaataccat atcaatttct
      961 gttgcaccat ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt tgtgcatgca
     1021 cctagaggaa aacctacaac atttgttatt cctacatttg tgccttttaa taattcttta
     1081 caatagcttg ttcaatatga attaacacaa actgttgcaa aatcaaattc aattgcttca
     1141 tcacataatt gtttaatttc agctttcgta gcatcttgtt ttaataatgt gtgatctata
     1201 tattcgttta gtttcatttt ttctcctata tattcatttt taattttaat tctttaataa
     1261 tttcgtctac tttaacttta gcgttttgaa cagattcacc aacacctata aaataaattt
     1321 ttagtttagg ttcagttcca cttgggcgaa cagcaaatca tgacttatct tctaaataaa
     1381 attttagtaa gtcttgtcct ggcatattat acattccatc gatgtagtct tcaacattaa
     1441 caactttaag tccagcaatt tgagttaagg gtgttgctct caatgatttc attaatggtt
     1501 caatttttaa tttcttttct tctggtttaa aattcaagtt taaagtgaaa gtgtaatatg
     1561 cacccatttc tttaaataaa tcttctaaat agtctactaa tgttttattt tgttttttat
     1621 aaaatcaagc agcctctgct attaatatag aagcttgtat tccatcttta tctctagctg
     1681 agtcatcaat tacatatcca taactttctt cataagcaaa aacaaaattt aatccgttat
     1741 cttcttcttt agcaatttct ctacccattc atttaaatcc agttaaagtt tttacaatat
     1801 taactccata tttttcatga gcgattctat cacccaaatc acttgttaca aaacttgaat
     1861 atagagccgg attttttgga atgctattta agcgttttag atttgataat tttcaatcaa
     1921 ttaaaattgg tcctgtttga tttccatcta atcttacaaa atgaccatca tgttttattg
     1981 ccattccaaa tctgtcagca tctgggtcat tcataataat aatatctgca tcatgtttaa
     2041 taccatattc aagcggtatt tttcatgcag gatcaaattc tggatttgga tttacaacat
     2101 ttttaaatgt ttcatcttca aatgcatgct cttcaacctc aataacgtta tatcctgatt
     2161 cacgtaatat ttttggggta aatttagttc ctgttccatt aactgcgcta aaaataattt
     2221 ttaaatcttt tttagcttct tgctcttttt tgtacgtctc tgtttaagag ctatgctgga
     2281 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     2341 tcggtgcata ggcaggtgta taatagcacc tgctatacgc ggttctatct agttacgcgt
     2401 taaaccaact agaatttttt gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2461 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2521 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2581 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2641 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggtgc caccatggcc
     2701 ccaaagaaga agcggaaggt cggtgacaag aagtacagca tcggcctgga catcggcacc
     2761 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
     2821 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
     2881 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
     2941 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
     3001 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
     3061 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
     3121 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
     3181 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
     3241 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
     3301 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
     3361 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
     3421 aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
     3481 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
     3541 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
     3601 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
     3661 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
     3721 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
     3781 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
     3841 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
     3901 aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
     3961 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
     4021 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
     4081 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
     4141 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
     4201 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
     4261 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
     4321 atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc
     4381 aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag
     4441 tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca
     4501 taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag
     4561 gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag
     4621 gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg
     4681 cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag
     4741 cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc
     4801 atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg
     4861 tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt
     4921 aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg
     4981 cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga
     5041 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
     5101 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
     5161 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
     5221 tccgactacg atgtggacgc tatcgtgcct cagagctttc tgaaggacga ctccatcgac
     5281 aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa
     5341 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
     5401 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
     5461 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
     5521 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
     5581 aaagtgatca ccctgaagtc caagctggtg tccggtttcc ggaaggattt ccagttttac
     5641 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
     5701 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac
     5761 aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc
     5821 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
     5881 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
     5941 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
     6001 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
     6061 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
     6121 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
     6181 tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc
     6241 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
     6301 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
     6361 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
     6421 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
     6481 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
     6541 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
     6601 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
     6661 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
     6721 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
     6781 ggcctgtacg agacacggat cgacctgtct cagctgggag gtgactctgg aggatctagc
     6841 ggaggatcct ctggcagcga gacaccagga acaagcgagt cagcaacacc agagagcagt
     6901 ggcggcagca gcggcggcag cagcacccta aatatagaag atgagtatcg gctacatgag
     6961 acctcaaaag agccagatgt ttctctaggg tccacatggc tgtctgattt tcctcaggcc
     7021 tgggcggaaa ccgggggcat gggactggca gttcgccaag ctcctctgat catacctctg
     7081 aaagcaacct ctacccccgt gtccataaaa caatacccca tgtcacaaga agccagactg
     7141 gggatcaagc cccacataca gagactgttg gaccagggaa tactggtacc ctgccagtcc
     7201 ccctggaaca cgcccctgct acccgttaag aaaccaggga ctaatgatta taggcctgtc
     7261 caggatctga gagaagtcaa caagcgggtg gaagacatcc accccaccgt gcccaaccct
     7321 tacaacctct tgagcgggct cccaccgtcc caccagtggt acactgtgct tgatttaaag
     7381 gatgcctttt tctgcctgag actccacccc accagtcagc ctctcttcgc ctttgagtgg
     7441 agagatccag agatgggaat ctcaggacaa ttgacctgga ccagactccc acagggtttc
     7501 aaaaacagtc ccaccctgtt taatgaggca ctgcacagag acctagcaga cttccggatc
     7561 cagcacccag acttgatcct gctacagtac gtggatgact tactgctggc cgccacttct
     7621 gagctagact gccaacaagg tactcgggcc ctgttacaaa ccctagggaa cctcgggtat
     7681 cgggcctcgg ccaagaaagc ccaaatttgc cagaaacagg tcaagtatct ggggtatctt
     7741 ctaaaagagg gtcagagatg gctgactgag gccagaaaag agactgtgat ggggcagcct
     7801 actccgaaga cccctcgaca actaagggag ttcctaggga aggcaggctt ctgtcgcctc
     7861 ttcatccctg ggtttgcaga aatggcagcc cccctgtacc ctctcaccaa accggggact
     7921 ctgtttaatt ggggcccaga ccaacaaaag gcctatcaag aaatcaagca agctcttcta
     7981 actgccccag ccctggggtt gccagatttg actaagccct ttgaactctt tgtcgacgag
     8041 aagcagggct acgccaaagg tgtcctaacg caaaaactgg gaccttggcg tcggccggtg
     8101 gcctacctgt ccaaaaagct agacccagta gcagctgggt ggcccccttg cctacggatg
     8161 gtagcagcca ttgccgtact gacaaaggat gcaggcaagc taaccatggg acagccacta
     8221 gtcattctgg ccccccatgc agtagaggca ctagtcaaac aaccccccga ccgctggctt
     8281 tccaacgccc ggatgactca ctatcaggcc ttgcttttgg acacggaccg ggtccagttc
     8341 ggaccggtgg tagccctgaa cccggctacg ctgctcccac tgcctgagga agggctgcaa
     8401 cacaactgcc ttgatatcct ggccgaagcc cacggaaccc gacccgacct aacggaccag
     8461 ccgctcccag acgccgacca cacctggtac acggatggaa gcagtctctt acaagaggga
     8521 cagcgtaagg cgggagctgc ggtgaccacc gagaccgagg taatctgggc taaagccctg
     8581 ccagccggga catccgctca gcgggctgaa ctgatagcac tcacccaggc cctaaagatg
     8641 gcagaaggta agaagctaaa tgtttatact gatagccgtt atgcttttgc tactgcccat
     8701 atccatggag aaatatacag aaggcgtggg tggctcacat cagaaggcaa agagatcaaa
     8761 aataaagacg agatcttggc cctactaaaa gccctctttc tgcccaaaag acttagcata
     8821 atccattgtc caggacatca aaagggacac agcgccgagg ctagaggcaa ccggatggct
     8881 gaccaagcgg cccgaaaggc agccatcaca gagactccag acacctctac cctcctcata
     8941 gaaaattcat caccctctgg cggctcaaaa agaaccgccg acggcagcga attcgagccc
     9001 aagaagaaga ggaaagtcgg atccggcgca acaaacttct ctctgctgaa acaagccgga
     9061 gatgtcgaag agaatcctgg accgaccgag tacaagccca cggtgcgcct cgccacccgc
     9121 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     9181 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     9241 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     9301 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     9361 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     9421 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt ctcgcccgac
     9481 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     9541 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     9601 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     9661 acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     9721 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     9781 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     9841 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     9901 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     9961 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
    10021 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
    10081 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
    10141 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
    10201 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
    10261 tgggccgcct ccccgcacgc gtaataaaag atctttattt tcattagatc tgtgtgttgg
    10321 ttttttgtgt gctctagatt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10381 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10441 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10501 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10561 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10621 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10681 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10741 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10801 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10861 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10921 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10981 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    11041 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    11101 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    11161 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    11221 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11281 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11341 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11401 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11461 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11521 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11581 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11641 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11701 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11761 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11821 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11881 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11941 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    12001 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    12061 ggcggagcct atggaaaaac gccac
//
