LOCUS       sequence-42547-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Iacovino M, Bosnakovski D, Fey H, Rux D, Bajwa G, Mahen E, Mitanoska
            A, Xu Z, Kyba M
  TITLE     Inducible cassette exchange: a rapid and efficient system enabling 
            conditional gene expression in embryonic stem and primary cells.
  JOURNAL   Stem Cells. 2011 Oct;29(10):1580-8.
  PUBMED    22039605
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem 
            Cells."; date: "2011-10"; volume: "29(10)"; pages: "1580-8"
COMMENT     Sequence Label: p2Lox
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(41..57)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(41..57)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    81..202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(92..111)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     146..165
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      385..840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(472..491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     682..703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tgtcagtaat gaattaatta agaattcctg cagcccgggg gatccactag ttctagagcg
       61 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      121 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      181 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      241 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcgc
      301 gataaggatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      361 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      421 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      481 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      541 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      601 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      661 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      721 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
      781 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      841 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt cacaccgcat atggtgcact
      901 ctcagtac
//
