LOCUS       sequence-42548-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00359
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             513..530
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gctttcttat
       61 aatgccaact ttgtacaaga aagttggcaa gtgttttcgt ttcttgcccg ctttctgtgg
      121 tctaccgcct ttggccgctg gtttgccggc gaacttcttg cccttgtagg atttggagtt
      181 cgatcgtcgg ttttcttccc atttcaattg tttgtcggag gacttgtcct tgttaaagac
      241 ggcattgctg gttccctgac cctttacctt ggccgtctcc tccagcagcg aagagaagtc
      301 atcggcggca gcaaacagag aggacatatc gtgactctgt ttgagtgttt tggcaaagct
      361 tcgtcctccc accatgtcgg tggagtcttt gcgggatttt ttggatctcg gtggtgcatc
      421 ttcgtcgtcg tcatcctctt cgtctggact ctcaggagca tcgcttgaat cactgtcgcc
      481 agggccgcct tctgaaatcg agccctcatc gtcatcatca tcatcatcat cgtcgtcgtc
      541 atcaagtggc tgcaaatcaa tcgagcccgt ttcatcatcg gactgatctt cgccctcaat
      601 ttcatcatct tcatcttctg ccaagtcgtc atcaccccag tcgtcatcaa tatcgtccat
      661 ttcatcttcc gccttgtcgg actgcttctt ctttttctta tccttggact tatccttctt
      721 tatctcgcca cccagctcct gcaggaagtt cagctcctct tcatcctgct cttcgtcgac
      781 accttccttg
//
