LOCUS       sequence-42550-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00368
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(528..627)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(644..663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(645..663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(668..684)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(668..684)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttttttttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
       61 aatgccaact ttgtacaaaa aagttggcac catgtcggac tgggactctg tgactgttct
      121 tcggaagaaa gcaccgaaat cgtcgaccct caagacggaa tcggcggtca accaggcacg
      181 ccgtcaagga gtcgccgtgg atacccaaca gaagtatggt gctggcacca acaagcagca
      241 tgtgaccacc aagaacactg ccaaactaga ccgcgagacc gaggagctgc gccacgacaa
      301 gattcccctc gatgtgggca agctcatcca gcagggacga cagagcaagg gcctcagcca
      361 aaaggacctg gcaactaaaa tctgcgaaaa gcagcaagtg gtaaccgact acgaggccgg
      421 tcgcggtatt ccaaacaact tgattctggg taaaatggaa cgcgttctcg gcattaagct
      481 gcgcggcaag gagcgcggcc aaccaatagc acctcccggt aaaaagttgc caactttctt
      541 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
      601 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
      661 ttacatggtc atagctgttt cctggcagct ctggnccgtg tctcaaaatc tctgatgtta
      721 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
//
