LOCUS       sequence-42559-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) 
            neuronal inhibitor, with IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..33
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    96..129
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taacttcgta taaagtatcc tatacgaagt tatatcaaaa taggaagacc aatgcttcac
       61 catcgacccg aattgccaag catcaccatc gacccataac ttcgtataat gtatgctata
      121 cgaagttata ctagcgcgtg atatcgaact tactggttgt gtacgtcctc ccttcgcaca
      181 atcttgtaga tgatccaata aaacatgttg aaaatcagga aagccatggg gaatccgatc
      241 cgggagattt tgtcgatctt tttcgctctc tgaataaaga gtttgcgcat ttcctccggg
      301 ctcttagatg gagcaggggg tgggttggtt gtgttgctat tgttggctcc cttcacggaa
      361 attccgtctt tagcctggag gcaagcaggt cccataccat aggcgctaaa gttgaagcgt
      421 ccttctcctg cctcatcctc tttgtggtgg cgtcgttttc ttctgaaccg caacagctct
      481 ttatgctgtc gagacacaaa attgacagcg gcgtattcca gcagtgcgct gaagacaaac
      541 agaaggcaga cagccatcca gatatcgatg gccttcacgt aagaaacctt gggcaagctg
      601 gctcggctgc ctgagctctg agtagtcatt gtcagcactg tggtgatgcc cagtccgacc
      661 ctagctggag cggcgtccat attaatccag aaggagatcc atgacaaaat cactataagc
      721 aagcttggga tatacatttg gatcagataa taacccatgc gtctccgcat ggtcactgtg
      781 aaggtgacat ctgggtaggg ctctttgcag cattcataaa atcgctcgct tcttttaccg
      841 gggattccca caaggtcc
//
