LOCUS       sequence-425592-       12063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression in mammalian cells of the proximity
            ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear 
            exclusion sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG
  TITLE     Cooperative polarization of MCAM/CD146 and ERM family proteins in 
            melanoma.
  JOURNAL   Mol Biol Cell. 2023 Dec 20:mbcE23060255. doi: 
            10.1091/mbc.E23-06-0255.
  PUBMED    38117590
REFERENCE   2  (bases 1 to 12063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2023 Dec 20:mbcE23060255. doi: 10.1091/mbc.E23-06-0255."
COMMENT     Sequence Label: BirA*-HA-NES
FEATURES             Location/Qualifiers
     source          1..12063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             831..1011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1058..1183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2411..2528
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2584..3746
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2815..3737
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3694..3714
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3771..3795
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3921..4271,4272..4274,4275..4883)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             4887..4913
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4920..4946
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    complement(5013..5037)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5093..5667,5668..5670,5671..5679)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5260..5277)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5487..5506
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5705..6721
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5705..6721
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    6757..7345
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6810..6830)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7228..7239)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7864..8044
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8070..8087)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8076..8300
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8346..8774
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8433..8452)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8643..8664
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8783..8803)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8788..9117
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8968..9103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9030..9049
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9165..9212
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9231..9605
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9735..9856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9772..9791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9826..9845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9905..9921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9905..9921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9918..9940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9929..9945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9953..9959,9960..9977,9978..9983))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9998..10019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10136..10153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10307..10895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10387..10406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11066..11857,11858..11926))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11689..11708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11927..12031)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
      841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1021 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1081 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1141 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1201 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1261 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1321 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1381 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1441 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1501 taagaccacc gcacagcaag ggccgctgat cttcagacct ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatctacaa atggcagtat
     2401 tcatccacaa ttttaaaaga aaagggggga ttgggaggta cagtgcaggg gaaagaatag
     2461 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     2521 aaaattttcg ggtttattac agggacagca gaaattcact ttgaattaat tcaagcttcg
     2581 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     2641 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     2701 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     2761 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     2821 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     2881 attacttcca cctggctcca gtacgtgatt cttgatcccg agctggagcc aggggcgggc
     2941 cttgcgcttt aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     3001 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     3061 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     3121 taaatgcggg ccaggatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     3181 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     3241 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     3301 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     3361 aaagatggcc gcttcccggc cctgctccag ggggctcaaa atggaggacg cggcgctcgg
     3421 gagagcgggc gggtgagtca cccacacaaa ggaaaggggc ctttccgtcc tcagccgtcg
     3481 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctggagct
     3541 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     3601 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     3661 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     3721 tttttcttcc atttcaggtg tcgtgaacac gctaccggtc tcgagaattg acaagtttgt
     3781 acaaaaaagc aggctccgct agcgctaccg gactcagatc tcgagctcaa gcttggtacc
     3841 gagctcggat cgtcgctagc gatatcggcg cgccagcatt taaatctgta cagaccggtg
     3901 aattcggatc cggtggaggt atgaaggaca acaccgtgcc cctgaagctg atcgccctgc
     3961 tggccaacgg cgagttccac tctggcgagc agctgggaga gaccctggga atgagcagag
     4021 ccgccatcaa caagcacatc cagacactga gagactgggg agtggacgtg ttcaccgtgc
     4081 ctggcaaggg ctacagcctg cctgagccta tccagctgct gaacgccaag cagatcctgg
     4141 gacagctgga tggcggaagc gtggccgtgc tgcctgtgat cgactccacc aatcagtacc
     4201 tgctggacag aatcggagag ctgaagtccg gcgacgcctg catcgccgag taccagcagg
     4261 ctggcagagg aggcagagga cggaagtggt tcagcccatt cggagccaac ctgtacctgt
     4321 ccatgttctg gagactggag cagggacctg ctgctgccat cggactgagt ctggtgatcg
     4381 gaatcgtgat ggccgaggtg ctgagaaagc tgggagccga caaggtgaga gtgaagtggc
     4441 ctaatgacct gtacctccag gaccgcaagc tggctggcat cctggtggag ctgacaggca
     4501 agacaggcga tgccgctcag atcgtgatcg gagccggaat caacatggcc atgagaagag
     4561 tggaggagag cgtggtgaac cagggctgga tcaccctgca ggaggctggc atcaacctgg
     4621 accggaacac cctggccgcc atgctgatca gagagctgag agccgctctg gagctgttcg
     4681 agcaggaggg actggctcct tacctgagca gatgggagaa gctggacaac ttcatcaaca
     4741 gacctgtgaa gctgatcatc ggcgacaagg aaatcttcgg catctccaga ggaatcgaca
     4801 agcagggagc tctgctgctg gagcaggacg gaatcatcaa gccctggatg ggcggagaaa
     4861 tctccctgag aagcgcagag aaggcttacc cttacgatgt accggattac gcactgcagc
     4921 tgcctcccct ggagcgcctg accctggact aagcggccgc tcgacggtac cgcgggcccg
     4981 ggatcggccg cgactctaga tcataatcag ccacccagct ttcttgtaca aagtggtttg
     5041 attcgacccg ggatcgggcc gcactagagg aattccgccc ctctcccccc cccccctctc
     5101 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5161 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5221 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5281 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5341 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5401 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5461 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5521 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5581 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5641 ttttcctttg aaaaacacga tgataagctt gccacaaccc gtaccaaaga tggatagatc
     5701 cggaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     5761 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     5821 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     5881 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     5941 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     6001 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     6061 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     6121 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     6181 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     6241 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     6301 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     6361 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     6421 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     6481 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     6541 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     6601 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     6661 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     6721 agctagagtt aacatcgagg gatcaagctt atcgataatc aacctctgga ttacaaaatt
     6781 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6841 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6901 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6961 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7021 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7081 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7141 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7201 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7261 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7321 atctcccttt gggccgcctc cccgcatcga taccgtcgag acctagaaaa acatggagca
     7381 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     7441 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     7501 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7561 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7621 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     7681 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     7741 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     7801 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     7861 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7921 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7981 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8041 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8101 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8161 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8221 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8281 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     8341 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8401 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8461 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8521 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8581 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8641 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8701 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8761 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8821 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8881 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8941 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9001 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     9061 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9121 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     9181 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     9241 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     9301 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     9361 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     9421 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     9481 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     9541 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     9601 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     9661 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     9721 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9781 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9841 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     9901 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9961 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    10021 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    10081 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    10141 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    10201 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    10261 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10321 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10381 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10441 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10501 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10561 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10621 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10681 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10741 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10801 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10861 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10921 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10981 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    11041 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    11101 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    11161 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    11221 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    11281 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    11341 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    11401 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    11461 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    11521 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    11581 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    11641 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11701 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11761 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11821 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11881 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11941 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    12001 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    12061 tcg
//
