LOCUS       sequence-425597-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP expressing shRNA targeting Cdh2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Tan CX, Bindu DS, Hardin EJ, Sakers K, Baumert R, Ramirez JJ, Savage
            JT, Eroglu C
  TITLE     delta-Catenin controls astrocyte morphogenesis via layer-specific 
            astrocyte-neuron cadherin interactions.
  JOURNAL   J Cell Biol. 2023 Nov 6;222(11):e202303138. doi: 
            10.1083/jcb.202303138. Epub 2023 Sep 14.
  PUBMED    37707499
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-11-6"; volume: "222(11):e202303138. doi"; pages:
            " 10.1083/jcb.202303138. Epub 2023 Sep 14"
COMMENT     Sequence Label: pLKO.1-shCdh2.1 GFP
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    37..625
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(90..110)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(508..519)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(628..644)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(629..645)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             834..1067
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1139..1260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1176..1195)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1230..1249
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1300..1435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1455..1474)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1456..1474)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1484..1501)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1484..1500)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1493..1515)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1642..2097
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1729..1748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1939..1960
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2123..2227
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2228..2296,2297..3088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2446..2465)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3259..3847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3748..3767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4001..4018
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4135..4156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4171..4176,4177..4194,4195..4201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4209..4225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4214..4236
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4233..4249
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4233..4249
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4268..4288
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4270..4288
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4316..4542
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4543..4723
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4770..4895
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5388..5621
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5806..5850
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6148..6396
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6148..6168
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6319..6338
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    6493..6610
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6977..7280
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7282..7558
                     /label=chicken beta-actin promoter
     intron          7559..8575
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8499..8521
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8583..8602
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      8652..8661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8658..8660,8661..8663,8664..9377)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8658..8660,8661..8663,8664..9374)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8703..8724)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8964..8983)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9311..9332
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(9397..9430)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttccactagg tacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
       61 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
      121 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
      181 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
      241 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
      301 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
      361 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
      421 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
      481 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
      541 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
      601 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa
      661 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
      721 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
      781 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
      841 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
      901 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      961 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1021 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     1081 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     1141 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1201 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1261 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     1321 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     1381 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     1441 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     1501 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1561 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1621 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1681 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1741 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1801 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     1861 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1921 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1981 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     2041 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     2101 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2161 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2221 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2281 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2341 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2401 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2461 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2521 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2581 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2641 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2701 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2761 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2821 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2881 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2941 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     3001 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     3061 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     3121 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3181 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3241 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3301 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3361 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3421 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3481 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3541 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3601 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3661 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3721 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3781 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3841 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3901 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3961 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4021 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     4081 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     4141 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     4201 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     4261 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     4321 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     4381 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     4441 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     4501 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     4561 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4621 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4681 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     4741 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     4801 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     4861 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     4921 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     4981 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     5041 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     5101 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     5161 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     5221 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     5281 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     5341 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     5401 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     5461 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     5521 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     5581 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     5641 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     5701 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     5761 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     5821 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     5881 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     5941 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     6001 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     6061 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac
     6121 tagcctcgag cggccgcccc cttcaccgag ggcctatttc ccatgattcc ttcatatttg
     6181 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     6241 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     6301 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     6361 ttggctttat atatcttgtg gaaaggacga aacaccgggc tgtagttaga atactcaatc
     6421 tcgagattga gtattctaac tacagctttt tgaattctcg acctcgagac aaatggcagt
     6481 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     6541 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     6601 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg
     6661 ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     6721 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     6781 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     6841 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     6901 aagccgcacg tctcactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     6961 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7021 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7081 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7141 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7201 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7261 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     7321 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     7381 tgggggcggg gggggggggg ggggcgcgcc aggcggggcg gggcggggcg aggggcgggg
     7441 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     7501 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     7561 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     7621 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     7681 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     7741 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     7801 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     7861 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     7921 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     7981 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     8041 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     8101 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     8161 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     8221 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     8281 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     8341 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     8401 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     8461 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     8521 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     8581 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg cggccgctag
     8641 cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     8701 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     8761 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     8821 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     8881 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     8941 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     9001 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     9061 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     9121 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     9181 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     9241 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     9301 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     9361 acgagctgta caagtaggaa ttcgtcgagg gacctaataa cttcgtatag catacattat
     9421 acgaagttat acatgtttaa gggttccgg
//
