LOCUS       sequence-425599-       13894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of MCAM-BirA*-HA fusion protein
            in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG
  TITLE     Cooperative polarization of MCAM/CD146 and ERM family proteins in 
            melanoma.
  JOURNAL   Mol Biol Cell. 2023 Dec 20:mbcE23060255. doi: 
            10.1091/mbc.E23-06-0255.
  PUBMED    38117590
REFERENCE   2  (bases 1 to 13894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2023 Dec 20:mbcE23060255. doi: 10.1091/mbc.E23-06-0255."
COMMENT     Sequence Label: MCAM-BirA*-HA
FEATURES             Location/Qualifiers
     source          1..13894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             831..1011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1058..1183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2411..2528
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2584..3746
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2815..3737
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3694..3714
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3771..3795
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(5788..6138,6139..6141,6142..6750)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             6754..6780
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6844..6868)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6924..7498,7499..7501,7502..7510)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7091..7108)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7318..7337
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7536..8552
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             7536..8552
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    8588..9176
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8641..8661)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9059..9070)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9695..9875
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9901..9918)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9907..10131
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10177..10605
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10264..10283)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10474..10495
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10614..10634)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10619..10948
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10799..10934
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10861..10880
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10996..11043
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11062..11436
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11566..11687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11603..11622)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11657..11676
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11736..11752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11736..11752)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11749..11771)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11760..11776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11784..11790,11791..11808,11809..11814))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11829..11850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11967..11984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12138..12726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12218..12237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12897..13688,13689..13757))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13520..13539
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13758..13862)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
      841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1021 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1081 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1141 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1201 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1261 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1321 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1381 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1441 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1501 taagaccacc gcacagcaag ggccgctgat cttcagacct ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatctacaa atggcagtat
     2401 tcatccacaa ttttaaaaga aaagggggga ttgggaggta cagtgcaggg gaaagaatag
     2461 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     2521 aaaattttcg ggtttattac agggacagca gaaattcact ttgaattaat tcaagcttcg
     2581 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     2641 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     2701 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     2761 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     2821 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     2881 attacttcca cctggctcca gtacgtgatt cttgatcccg agctggagcc aggggcgggc
     2941 cttgcgcttt aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     3001 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     3061 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     3121 taaatgcggg ccaggatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     3181 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     3241 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     3301 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     3361 aaagatggcc gcttcccggc cctgctccag ggggctcaaa atggaggacg cggcgctcgg
     3421 gagagcgggc gggtgagtca cccacacaaa ggaaaggggc ctttccgtcc tcagccgtcg
     3481 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctggagct
     3541 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     3601 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     3661 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     3721 tttttcttcc atttcaggtg tcgtgaacac gctaccggtc tcgagaattg acaagtttgt
     3781 acaaaaaagc aggctccgct agcgctaccg gactcagatc tcgagctcaa gcttatgggg
     3841 cttcccaggc tggtctgcgc cttcttgctc gccgcctgct gctgctgtcc tcgcgtcgcg
     3901 ggtgtgcccg gagaggctga gcagcctgcg cctgagctgg tggaggtgga agtgggcagc
     3961 acagcccttc tgaagtgcgg cctctcccag tcccaaggca acctcagcca tgtcgactgg
     4021 ttttctgtcc acaaggagaa gcggacgctc atcttccgtg tgcgccaggg ccagggccag
     4081 agcgaacctg gggagtacga gcagcggctc agcctccagg acagaggggc tactctggcc
     4141 ctgactcaag tcacccccca agacgagcgc atcttcttgt gccagggcaa gcgccctcgg
     4201 tcccaggagt accgcatcca gctccgcgtc tacaaagctc cggaggagcc aaacatccag
     4261 gtcaaccccc tgggcatccc tgtgaacagt aaggagcctg aggaggtcgc tacctgtgta
     4321 gggaggaacg ggtaccccat tcctcaagtc atctggtaca agaatggccg gcctctgaag
     4381 gaggagaaga accgggtcca cattcagtcg tcccagactg tggagtcgag tggtttgtac
     4441 accttgcaga gtattctgaa ggcacagctg gttaaagaag acaaagatgc ccagttttac
     4501 tgtgagctca actaccggct gcccagtggg aaccacatga aggagtccag ggaagtcacc
     4561 gtccctgttt tctacccgac agaaaaagtg tggctggaag tggagcccgt gggaatgctg
     4621 aaggaagggg accgcgtgga aatcaggtgt ttggctgatg gcaaccctcc accacacttc
     4681 agcatcagca agcagaaccc cagcaccagg gaggcagagg aagagacaac caacgacaac
     4741 ggggtcctgg tgctggagcc tgcccggaag gaacacagtg ggcgctatga atgtcagggc
     4801 ctggacttgg acaccatgat atcgctgctg agtgaaccac aggaactact ggtgaactat
     4861 gtgtctgacg tccgagtgag tcccgcagcc cctgagagac aggaaggcag cagcctcacc
     4921 ctgacctgtg aggcagagag tagccaggac ctcgagttcc agtggctgag agaagagaca
     4981 ggccaggtgc tggaaagggg gcctgtgctt cagttgcatg acctgaaacg ggaggcagga
     5041 ggcggctatc gctgcgtggc gtctgtgccc agcatacccg gcctgaaccg cacacagctg
     5101 gtcaacgtgg ccatttttgg ccccccttgg atggcattca aggagaggaa ggtgtgggtg
     5161 aaagagaata tggtgttgaa tctgtcttgt gaagcgtcag ggcacccccg gcccaccatc
     5221 tcctggaacg tcaacggcac ggcaagtgaa caagaccaag atccacagcg agtcctgagc
     5281 accctgaatg tcctcgtgac cccggagctg ttggagacag gtgttgaatg cacggcctcc
     5341 aacgacctgg gcaaaaacac cagcatcctc ttcctggagc tggtcaattt aaccaccctc
     5401 acaccagact ccaacacaac cactggcctc agcacttcca ctgccagtcc tcataccaga
     5461 gccaacagca cctccacaga gagaaagctg ccggagccgg agagccgggg cgtggtcatc
     5521 gtggctgtga ttgtgtgcat cctggtcctg gcggtgctgg gcgctgtcct ctatttcctc
     5581 tataagaagg gcaagctgcc gtgcaggcgc tcagggaagc aggagatcac gctgcccccg
     5641 tctcgtaaga gcgaacttgt agttgaagtt aagtcagata agctcccaga agagatgggc
     5701 ctcctgcagg gcagcagcgg tgacaagagg gctccgggag accagggaga gaaatacatc
     5761 gatctgaggc atggatccgg tggaggtatg aaggacaaca ccgtgcccct gaagctgatc
     5821 gccctgctgg ccaacggcga gttccactct ggcgagcagc tgggagagac cctgggaatg
     5881 agcagagccg ccatcaacaa gcacatccag acactgagag actggggagt ggacgtgttc
     5941 accgtgcctg gcaagggcta cagcctgcct gagcctatcc agctgctgaa cgccaagcag
     6001 atcctgggac agctggatgg cggaagcgtg gccgtgctgc ctgtgatcga ctccaccaat
     6061 cagtacctgc tggacagaat cggagagctg aagtccggcg acgcctgcat cgccgagtac
     6121 cagcaggctg gcagaggagg cagaggacgg aagtggttca gcccattcgg agccaacctg
     6181 tacctgtcca tgttctggag actggagcag ggacctgctg ctgccatcgg actgagtctg
     6241 gtgatcggaa tcgtgatggc cgaggtgctg agaaagctgg gagccgacaa ggtgagagtg
     6301 aagtggccta atgacctgta cctccaggac cgcaagctgg ctggcatcct ggtggagctg
     6361 acaggcaaga caggcgatgc cgctcagatc gtgatcggag ccggaatcaa catggccatg
     6421 agaagagtgg aggagagcgt ggtgaaccag ggctggatca ccctgcagga ggctggcatc
     6481 aacctggacc ggaacaccct ggccgccatg ctgatcagag agctgagagc cgctctggag
     6541 ctgttcgagc aggagggact ggctccttac ctgagcagat gggagaagct ggacaacttc
     6601 atcaacagac ctgtgaagct gatcatcggc gacaaggaaa tcttcggcat ctccagagga
     6661 atcgacaagc agggagctct gctgctggag caggacggaa tcatcaagcc ctggatgggc
     6721 ggagaaatct ccctgagaag cgcagagaag gcttaccctt acgatgtacc ggattacgca
     6781 taggcggccg ctcgacggta ccgcgggccc gggatcggcc gcgactctag atcataatca
     6841 gccacccagc tttcttgtac aaagtggttt gattcgaccc gggatcgggc cgcactagag
     6901 gaattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt tactggccga
     6961 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     7021 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     7081 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     7141 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7201 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7261 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     7321 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     7381 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     7441 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     7501 tgccacaacc cgtaccaaag atggatagat ccggaaagcc tgaactcacc gcgacgtctg
     7561 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     7621 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     7681 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     7741 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     7801 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     7861 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     7921 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     7981 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     8041 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     8101 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     8161 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     8221 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     8281 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     8341 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     8401 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     8461 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     8521 gacgccccag cactcgtccg agggcaaagg aagctagagt taacatcgag ggatcaagct
     8581 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     8641 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     8701 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     8761 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     8821 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     8881 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     8941 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     9001 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     9061 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     9121 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     9181 ataccgtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     9241 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     9301 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9361 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     9421 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     9481 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     9541 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     9601 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     9661 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     9721 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9781 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9841 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     9901 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     9961 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    10021 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    10081 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
    10141 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
    10201 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
    10261 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
    10321 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
    10381 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
    10441 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    10501 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    10561 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
    10621 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10681 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    10741 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    10801 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    10861 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
    10921 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
    10981 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
    11041 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
    11101 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
    11161 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
    11221 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
    11281 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
    11341 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
    11401 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
    11461 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
    11521 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
    11581 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11641 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
    11701 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    11761 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    11821 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    11881 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    11941 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    12001 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    12061 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    12121 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    12181 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    12241 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    12301 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    12361 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    12421 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    12481 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    12541 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    12601 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    12661 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    12721 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    12781 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    12841 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    12901 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    12961 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    13021 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    13081 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    13141 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    13201 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    13261 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    13321 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    13381 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    13441 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    13501 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    13561 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    13621 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    13681 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    13741 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    13801 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    13861 cgcacatttc cccgaaaagt gccacctgac gtcg
//
