LOCUS       sequence-425600-       12099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression in mammalian cells of the proximity
            ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane 
            localization sequence and C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12099)
  AUTHORS   Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG
  TITLE     Cooperative polarization of MCAM/CD146 and ERM family proteins in 
            melanoma.
  JOURNAL   Mol Biol Cell. 2023 Dec 20:mbcE23060255. doi: 
            10.1091/mbc.E23-06-0255.
  PUBMED    38117590
REFERENCE   2  (bases 1 to 12099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2023 Dec 20:mbcE23060255. doi: 10.1091/mbc.E23-06-0255."
COMMENT     Sequence Label: Lyn11-BirA*-HA
FEATURES             Location/Qualifiers
     source          1..12099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             831..1011
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1058..1183
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2411..2528
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2584..3746
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2815..3737
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3694..3714
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3771..3795
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3993..4343,4344..4346,4347..4955)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             4959..4985
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5049..5073)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5129..5703,5704..5706,5707..5715)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5296..5313)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5523..5542
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5741..6757
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5741..6757
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    6793..7381
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6846..6866)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7264..7275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7900..8080
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8106..8123)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8112..8336
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8382..8810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8469..8488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8679..8700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8819..8839)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8824..9153
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9004..9139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9066..9085
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9201..9248
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9267..9641
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9771..9892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9808..9827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9862..9881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9941..9957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9941..9957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9954..9976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9965..9981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9989..9995,9996..10013,10014..10019))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10034..10055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10172..10189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10343..10931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10423..10442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11102..11893,11894..11962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11725..11744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11963..12067)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
      841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1021 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1081 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1141 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1201 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1261 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1321 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1381 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1441 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1501 taagaccacc gcacagcaag ggccgctgat cttcagacct ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatctacaa atggcagtat
     2401 tcatccacaa ttttaaaaga aaagggggga ttgggaggta cagtgcaggg gaaagaatag
     2461 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     2521 aaaattttcg ggtttattac agggacagca gaaattcact ttgaattaat tcaagcttcg
     2581 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     2641 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     2701 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     2761 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     2821 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     2881 attacttcca cctggctcca gtacgtgatt cttgatcccg agctggagcc aggggcgggc
     2941 cttgcgcttt aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     3001 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     3061 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     3121 taaatgcggg ccaggatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     3181 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     3241 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     3301 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     3361 aaagatggcc gcttcccggc cctgctccag ggggctcaaa atggaggacg cggcgctcgg
     3421 gagagcgggc gggtgagtca cccacacaaa ggaaaggggc ctttccgtcc tcagccgtcg
     3481 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctggagct
     3541 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     3601 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     3661 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     3721 tttttcttcc atttcaggtg tcgtgaacac gctaccggtc tcgagaattg acaagtttgt
     3781 acaaaaaagc aggctccgct agcgctaccg gactcagatc tcgagctcaa gcttggtacc
     3841 gagctcggat cgtcgctagc gatatcggcg cgccagcatt taaatctgta cagaccggtg
     3901 aattcatggg atgtataaaa tcaaaaggga aagacagcgc gggagcagat agtgctggta
     3961 gtgctggtag tgctggtgga tccggtggag gtatgaagga caacaccgtg cccctgaagc
     4021 tgatcgccct gctggccaac ggcgagttcc actctggcga gcagctggga gagaccctgg
     4081 gaatgagcag agccgccatc aacaagcaca tccagacact gagagactgg ggagtggacg
     4141 tgttcaccgt gcctggcaag ggctacagcc tgcctgagcc tatccagctg ctgaacgcca
     4201 agcagatcct gggacagctg gatggcggaa gcgtggccgt gctgcctgtg atcgactcca
     4261 ccaatcagta cctgctggac agaatcggag agctgaagtc cggcgacgcc tgcatcgccg
     4321 agtaccagca ggctggcaga ggaggcagag gacggaagtg gttcagccca ttcggagcca
     4381 acctgtacct gtccatgttc tggagactgg agcagggacc tgctgctgcc atcggactga
     4441 gtctggtgat cggaatcgtg atggccgagg tgctgagaaa gctgggagcc gacaaggtga
     4501 gagtgaagtg gcctaatgac ctgtacctcc aggaccgcaa gctggctggc atcctggtgg
     4561 agctgacagg caagacaggc gatgccgctc agatcgtgat cggagccgga atcaacatgg
     4621 ccatgagaag agtggaggag agcgtggtga accagggctg gatcaccctg caggaggctg
     4681 gcatcaacct ggaccggaac accctggccg ccatgctgat cagagagctg agagccgctc
     4741 tggagctgtt cgagcaggag ggactggctc cttacctgag cagatgggag aagctggaca
     4801 acttcatcaa cagacctgtg aagctgatca tcggcgacaa ggaaatcttc ggcatctcca
     4861 gaggaatcga caagcaggga gctctgctgc tggagcagga cggaatcatc aagccctgga
     4921 tgggcggaga aatctccctg agaagcgcag agaaggctta cccttacgat gtaccggatt
     4981 acgcataggc ggccgctcga cggtaccgcg ggcccgggat cggccgcgac tctagatcat
     5041 aatcagccac ccagctttct tgtacaaagt ggtttgattc gacccgggat cgggccgcac
     5101 tagaggaatt ccgcccctct cccccccccc cctctccctc ccccccccct aacgttactg
     5161 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     5221 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     5281 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     5341 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     5401 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     5461 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     5521 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     5581 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     5641 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     5701 aagcttgcca caacccgtac caaagatgga tagatccgga aagcctgaac tcaccgcgac
     5761 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     5821 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     5881 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     5941 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     6001 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     6061 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     6121 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     6181 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     6241 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     6301 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     6361 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     6421 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     6481 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     6541 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     6601 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     6661 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     6721 aaaccgacgc cccagcactc gtccgagggc aaaggaagct agagttaaca tcgagggatc
     6781 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6961 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7021 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7081 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7141 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7201 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7261 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7321 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7381 catcgatacc gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7441 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7501 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7561 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7621 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     7681 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     7741 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     7801 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     7861 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     7921 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7981 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8041 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     8101 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     8161 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     8221 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     8281 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     8341 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     8401 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8461 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8521 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8581 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8641 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8701 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     8761 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     8821 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8881 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8941 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9001 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9061 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     9121 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     9181 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     9241 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     9301 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     9361 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     9421 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     9481 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     9541 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     9601 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     9661 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     9721 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     9781 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9841 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9901 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     9961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    10021 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    10081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    10141 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    10201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    10261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    10321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    10381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    10441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    10501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    10561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    10621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    10681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    10741 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    10801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    10861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    10921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    10981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    11041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    11101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    11161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    11221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    11281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    11341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    11401 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    11461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    11521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    11581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    11641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    11701 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    11761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    11821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    11881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    11941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    12001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    12061 ttccgcgcac atttccccga aaagtgccac ctgacgtcg
//
