LOCUS       sequence-425606-        4095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UBQ10 promoter in domestication vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pUPD2-pAtUBQ10 (A1-B1)
FEATURES             Location/Qualifiers
     source          1..4095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2052..2109
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2133..2150)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2304..2892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2384..2403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3075..3169)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3190..3849)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3764..3783
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     4011..4029
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4033..4076)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(4079..4095,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg ggagagtcta gctcaacaga gcttttaacc caaattggta
       61 caatagaata caactttaga tcataattct caaaagaaag agattcctta gctattctat
      121 ctgccactcc atttccttct cggcttgtat gcacaagcat aaaatcctca aacttgctaa
      181 gtagatactt tatgtcttgg ataattggat tgagacttga caagcataac tttcatgtaa
      241 ccaaagacac aagttgctga gaatccacct caaaaatgat cttcctataa ttgaatcggg
      301 ataatgacag cacagcccat ctaagagcct ccacttctac ttccagcacg cttcttactt
      361 ttaccacagc tcttgcacct aaccataaca ccttccctgt atgatcgcga agcacccacc
      421 ctaagccaca ttttaatcct tctgttggcc atgccccatc aaagttgcac ttaacccaag
      481 attgtggtgg agcttcccat gtttctcgtc tgtcccgacg gtgttgtggt tggtgctttc
      541 cttacattct gagcctcttt ccttctaatc cactcatctg catcttcttg tgtccttact
      601 aatacctcat tggttccaaa ttccctccct ttaagcacca gctcgtttct gttcttccac
      661 agcctcccaa gtatccaagg gactaaagcc tccacattct tcagatcagg atattcttgt
      721 ttaagatgtt gaactctatg gaggtttgta tgaactgatg atctaggacc ggataagttc
      781 ccttcttcat agcgaactta ttcaaagaat gttttgtgta tcattcttgt tacattgtta
      841 ttaatgaaaa aatattattg gtcattggac tgaacacgag tgttaaatat ggaccaggcc
      901 ccaaataaga tccattgata tatgaattaa ataacaagaa taaatcgagt caccaaacca
      961 cttgcctttt ttaacgagac ttgttcacca acttgataca aaagtcatta tcctatgcaa
     1021 atcaataatc atacaaaaat atccaataac actaaaaaat taaaagaaat ggataatttc
     1081 acaatatgtt atacgataaa gaagttactt ttccaagaaa ttcactgatt ttataagccc
     1141 acttgcatta gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat
     1201 tctagaaaat acgaaatacg cttcaatgca gtgggaccca cggttcaatt attgccaatt
     1261 ttcagctcca ccgtatattt aaaaaataaa acgataatgc taaaaaaata taaatcgtaa
     1321 cgatcgttaa atctcaacgg ctggatctta tgacgaccgt tagaaattgt ggttgtcgac
     1381 gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca
     1441 actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt
     1501 tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct
     1561 tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac
     1621 ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc
     1681 tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct
     1741 tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa
     1801 tcttagatcg aagacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta
     1861 gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc
     1921 ttcgatttgt gatttctatc tagatctggt gttagtttct agtttgtgcg atcgaatttg
     1981 tcgattaatc tgagtttttc tgattaacag ccattgagac cactcatcgc gactagtagc
     2041 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
     2101 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     2161 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2221 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2281 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     2341 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2461 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2701 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2761 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2821 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2881 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2941 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3061 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     3121 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     3181 gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat
     3241 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     3301 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     3361 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa
     3421 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     3481 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga
     3541 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     3601 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     3661 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     3721 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     3781 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     3841 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     3901 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag
     3961 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4021 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
     4081 attcgcggcc gcttc
//
