LOCUS       sequence-425610-        5622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-ROR2
FEATURES             Location/Qualifiers
     source          1..5622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2411..2510)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2526..2543)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2526..2542)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2535..2557)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2606..2692
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2784..2811
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2970..2987)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3141..3729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3221..3240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3822..4613)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     5044..5060
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5044..5060
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5065..5084
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5065..5083
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5101..5200
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gggatgaact ggggctgtgg gaagggcggg gccttctgct tgggccccac gtagcgggcg
       61 ttgctcacat tgctcactgg gctggtgctc agggagctgg tctgcgtggt gttgctggcc
      121 cccgaggtct gcgccgagct gttgtagttg gaaaggttgc cccaggctcg gagccggctg
      181 tggatgtcct tgaagcgggg ccgccggctg gggaactcgt tccagcactc gatcatgagg
      241 gcatacaccc aggcgggaca gtcatcgggg caaggcagca cctgccggtt ccggatcatc
      301 tccaccacat cctggttgga atacccgcag tagggctgca ggccgtagct gaagacctcc
      361 cacaggacca caccgtagga ccagatgtct gagtcgatgg agaacttgcc gtacatgatg
      421 gcctctgggg ccatccagcg gataggcagc agcgagttcc ccagcagctt gtagtaatcg
      481 gcggcataca cctctcggaa gaggcccaag tctgagatct tcacgttcag cttgtcgtac
      541 actagcacat tgcgggtggc caggtccttg tgaaccacgt ggtggctgga taggtactcc
      601 atccccgccg cgatctgtgc cacaaggtgc acgaagtcgg ggggctccag ggcggacttc
      661 accgtgcggt catcatcggt gctgcccacg tccgagtgcg gcgagcgcat gaccaggaat
      721 tcgtggaggt cgccgtgcga acagtagctg aagatcatgc tcaggggctg gtccttggtc
      781 accacgccca gcaggcagac gacgttgggg tgttgcagcc gtgctcgcag catagcctca
      841 tgccggaact cctcccgcag gggcccctcc gctttgtcct tcagcgtttt gatggccaca
      901 gcctgggtct gctcccccgg ggcagggccg aacaggtgac ctttgtagac tttcccaaac
      961 cggtcctctc ccagctcctc catgaacctc accgcagaca ggctgatctc tttgagtttg
     1021 gcctgtttgt gctggttaat gaggggcatt tccatgtctt ggctgggcga ggccatcagc
     1081 tgtcgccgct gcggtgtgga cgcagatgcc ttctgcttat tccggcacat gcaaaccaag
     1141 aagaaaaggc aagcgatgac cagtggaatt gcgatgctgg ggaccaagat gtacagaatc
     1201 cccatcttgc tgctgtctcg gggactacac gagggtacgt cacacagttc catgcgtacg
     1261 tttttattct gcgtaaagca ccaggggccc tccatctggc ctccggggtt ccggcagtag
     1321 gcgtgccccc ctccaagctc agggaagtct gtgctggaca ggtggtggct gtgggggtgc
     1381 tgcagggccc acggctggca ctggtggcct gacttggtgg tgcttgccgt tcctctgtaa
     1441 tccatgcctg agccgttata gcactgatgg tagcggccca gcctctcggc tgggatgcca
     1501 atgcgcatgc agttggcagc gtcggggctc tcaggcatgg gcagcgcctc acacttgggc
     1561 agctgaagcc gcatgaggat gagcgggttg gagcgggcga tggtgtactc ctggcggcac
     1621 aggtcgctct ccagcacctc gcactcgtcg cggcacagct cacgcggctt gggtgcccgg
     1681 gagcgcgcgt cgcacagagg aaacacgaag tggcagaagg atgggatggc gaactgtgag
     1741 cactggtccg acaggtgcgt agacgtgccg atcatggtga aggccgctgt gattcggttt
     1801 tcaatctccc cctgcatctg aagcgagtcc acataaatgg tccggttgcc aatgaagcgt
     1861 gcacaggcaa ttccccggta aggctggcag aacccatcct cgtggtaatc atcctgaaag
     1921 ttatgatttg ggctgtgcgt tggacccagc cgcacaaaca ggacgccagt ggcggtaatg
     1981 gtcttcatcc cgttggtggc cacgcactgg tagtagccag tgtctgtcgt gtccaggtcc
     2041 tggattcgca gtcgtgaacc atattctgtc ttccggatga tgatccgccg cggctcctgc
     2101 accaccgggg catcattctt tagccaccgc acgttagggg gtgggtttcc tgccaccttg
     2161 cagtgcagaa ttgccgtctg gccttggaca atggtgatat tgtttactgg ctccagaaaa
     2221 ttcagaaagt aacctttcag agttggaatc gggccgtcct gcccatcaag gggtcctaaa
     2281 gggtcgttcg gatccagaac ctccacttca cctgaagtcc gggacactga gagcagaagc
     2341 gcggcggccg cccagacggc cgggatgcac agcagcggcc gccgcgggag cgccgagccc
     2401 cgggccatgg tgccaacttt tttgtacaaa gttggcatta taaaaaagca ttgctcatca
     2461 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gggcccgagc
     2521 ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc tagcgttaac
     2581 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc
     2641 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     2701 agcggatttg aacgttgtga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     2761 aactgccagg catcaaacta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     2821 acaaactctt cctggctagc ggtacgcgta ttaattgcgt tgcgctcact gcccgctttc
     2881 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2941 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3001 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3061 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3121 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3181 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3241 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3301 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3361 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3421 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3481 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3541 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3601 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3661 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3721 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg
     3781 taactcacgt taagggattt tggtcatggg tggctcgagg gttatttgcc gactaccttg
     3841 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     3901 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     3961 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     4021 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     4081 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     4141 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     4201 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     4261 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     4321 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     4381 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     4441 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     4501 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     4561 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct cataatgttt
     4621 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     4681 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     4741 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     4801 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     4861 gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     4921 gcaaccttgg gtagcagcga agtcgaggca tttctgtcct ggctggtcta gaggaggcat
     4981 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc
     5041 tgccaggaaa cagctatgac catgtaatac gactcactat aggggatatc agctggatgg
     5101 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
     5161 tgctttctta taatgccaac tttgtacaag aaagttggca aagcttccag ctggacttgg
     5221 gcctcgtcca cctgcagagt gtcacagtcc cccagcagct cagtctctgg gacagagcct
     5281 tcctcctcct cctctgcttc ctgcacggtg ctctgggccc catcttctgg ggcgttctgt
     5341 gtgtcatcag cgccctctga gagcagggct gccctgtctg ccatggatgt gttggagggg
     5401 gccgtggtga cgtagcctgt gctggtggag ccactgccac tgtggtgtga gctgggcttg
     5461 gggaccatct gaggaggcac ctgctgcggg gccatctgca ttgggatctg caccgggtag
     5521 aagttgggca ggtaggcccc ataggccggc accggctggt agccgttgac ggggatgtag
     5581 agctgcggcg ggggcaccat gggtctgatc tggcccttca tg
//
