LOCUS       sequence-425630-       13541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For microglia differentiation from iPSC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13541)
  AUTHORS   Drager NM, Sattler SM, Huang CT, Teter OM, Leng K, Hashemi SH, Hong 
            J, Aviles G, Clelland CD, Zhan L, Udeochu JC, Kodama L, Singleton 
            AB, Nalls MA, Ichida J, Ward ME, Faghri F, Gan L, Kampmann M
  TITLE     A CRISPRi/a platform in human iPSC-derived microglia uncovers 
            regulators of disease states.
  JOURNAL   Nat Neurosci. 2022 Aug 11. pii: 10.1038/s41593-022-01131-4. doi: 
            10.1038/s41593-022-01131-4.
  PUBMED    35953545
REFERENCE   2  (bases 1 to 13541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2022 Aug 11. pii: 10.1038/s41593-022-01131-4. doi: 
            10.1038/s41593-022-01131-4."
COMMENT     Sequence Label: CYBL-SPI1-CEBPB-IRF5
FEATURES             Location/Qualifiers
     source          1..13541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1391..1409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1427..1445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1463..1481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1499..1517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1535..1553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1571..1589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1607..1625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1670..1694
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1777..1786
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2602..2655
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3718..3771
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    5285..5406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5322..5341)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5376..5395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5455..5471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5455..5471)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5468..5490)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5479..5495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5503..5509,5510..5527,5528..5533))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5548..5569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5686..5703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5995..6014
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6013..6068
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6114..6133
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(6224..6970)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7002..7021)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7029..8045
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7083..7105)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8046..8323)
                     /label=chicken beta-actin promoter
     enhancer        8325..8704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    8710..8934
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8923..8940
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    8956..8989
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(9265..9284)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9534..9552
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      9694..9703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             9741..10009
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(10022..10233)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    10269..10493
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(10498..11298)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPRIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10609..10628)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11219..11238
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(11293..11346)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    11370..11395
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    11396..11429
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(12287..12355,12356..13147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12505..12524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(13318..13541,1..365)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
       61 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc gaattcgccc ttagagcaga
      421 gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg tggacgagga
      481 agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa acccagaagc
      541 ccagagcagg gccttaggga agcgggacgg ctctgttgga gagtccagta ttgaattagt
      601 gatagataca cagaaaaaaa gcaaattgaa taggtgaatg gataaacaaa ctgtggaaca
      661 tccagacaat gaaatattca ccaataaaaa gaaatgagct atcaagccac attggctatg
      721 cccttcacat ttaaggagga accttaaatg catattgcca aattttaaaa gctagtctga
      781 aaatgctaca gactgtatga ttccaattat atgacatttt agaaaaggca aaactataga
      841 gatcagtggt tgccagcggc tcatggaggc aggatgaatt ggtggaacac agggatgctt
      901 tcagggtaat gaaaatatct tgtgtgatcc tgtaataatg gatacgtggc ataatgcatt
      961 tgttaaaacc tgtaaactag ccaggtatag tggctttttt gcacctatag tctcagctac
     1021 ttggtaggct gagacaggaa gatcacttga gcccaggact tcaagaccag cctgggtaat
     1081 atagtaagac cccatctcta ttttttaaag aaattaagaa agaaaagaaa cccatcaact
     1141 gtataacaca aagaatcgta atgtaaacta cagtcctgag taatgaaaca tttttaaaga
     1201 aataaataaa ttcaaggcaa ttattaactt caggaaaaac agtaaagtaa acataagtat
     1261 acaaatcaaa catggctcag ttgtgaaaat atttacaagg tcactttaag taacattgaa
     1321 tgttgatata atcccaaaat atatttatgt tggaaggatg aggagatatc tccaccccac
     1381 agtggtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     1441 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     1501 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     1561 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     1621 agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     1681 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt
     1741 accacttcct accctcgtaa acttaaggtt aattaagcca ccatggaagg attccccctg
     1801 gtgccccctc cctccgaaga tctcgtgccc tacgacaccg atctgtacca gagacagaca
     1861 catgagtatt acccctacct gtccagcgac ggagagtccc acagcgacca ctattgggat
     1921 tttcaccccc accatgtgca ctccgagttc gagagctttg ctgagaacaa cttcaccgag
     1981 ctccagtccg tgcagcctcc tcaactccag cagctctata gacacatgga gctggagcag
     2041 atgcatgtgc tggatacccc catggtgcct ccccacccta gcctgggaca ccaagtctcc
     2101 tacctgccca ggatgtgcct ccagtaccct tccctgtccc ccgctcagcc cagcagcgat
     2161 gaggaagaag gcgaaaggca gtcccctcct ctggaggtgt ccgacggaga ggctgatggc
     2221 ctcgagcctg gacccggact gctgcccggc gagaccggca gcaaaaagaa gattaggctg
     2281 taccagttcc tcctggatct gctcagatcc ggcgacatga aggacagcat ttggtgggtc
     2341 gacaaggaca agggcacctt tcagttctcc agcaagcaca aagaggctct ggctcacagg
     2401 tggggcatcc agaagggcaa caggaagaag atgacctatc agaagatggc cagggccctc
     2461 agaaactacg gcaagaccgg agaggtgaaa aaagtcaaga agaagctcac ataccaattc
     2521 tccggcgagg tgctgggaag aggaggactg gctgagagga gacaccctcc tcactccagg
     2581 atgcatagca ggggcagcgg cgagggaagg ggttctctgc tcacctgtgg agatgtcgag
     2641 gagaaccccg gccccatgca gaggctggtg gcttgggacc ctgcctgcct ccctctccct
     2701 cctccccctc ctgctttcaa gagcatggag gtcgccaact tttactacga ggccgattgt
     2761 ctggccgccg cttatggcgg aaaagctgcc cccgccgccc cccccgccgc cagacccggc
     2821 cccaggcccc ccgctggcga actgggaagc atcggcgacc acgagagggc catcgacttt
     2881 agcccctacc tggagcctct cggcgccccc caagcccccg cccctgccac agctaccgac
     2941 acattcgagg ccgccccccc tgcccccgct cccgcccccg cctccagcgg ccagcaccat
     3001 gactttctgt ccgacctgtt ctccgatgac tacggcggca aaaactgcaa gaaacccgcc
     3061 gagtacggct atgtgtccct gggcaggctg ggagccgcta aaggagccct gcaccctggc
     3121 tgttttgccc ctctgcaccc yccycccccc cccccccccc cccccgccga gctcaaagct
     3181 gagcctggct tcgagcccgc cgattgcaag agaaaagagg aggctggcgc ccctggagga
     3241 ggcgccggca tggctgctgg attcccctat gctctgaggg cctacctggg ctaccaagcc
     3301 gtccctagcg gaagcagcgg ctccctgtcc acatcctcct cctccagccc tcctggaaca
     3361 ccttcccccg ccgatgctaa agcccccccc acagcctgtt acgccggcgc cgctcccgcc
     3421 ccttcccaag tgaaaagcaa ggccaagaag accgtggaca agcactccga cgagtataaa
     3481 atcagaaggg aaagaaacaa catcgccgtc agaaagtcca gggataaggc caagatgagg
     3541 aacctcgaga cacagcacaa ggtgctcgag ctcaccgccg agaacgaaag actgcaaaag
     3601 aaagtcgagc agctctccag ggaactgtcc accctcagaa acctgttcaa acagctgccc
     3661 gagcccctcc tggccagcag cggccactgt ggaagcatgc attccagagg ttctggcgaa
     3721 ggaaggggca gcctgctgac atgcggcgac gtggaagaaa atcccggccc catgaatcag
     3781 agcattcccg tggcccctac cccccctaga agagtcagac tgaaaccttg gctcgtcgcc
     3841 caggtgaata gctgtcagta ccctggcctg caatgggtga acggagagaa gaagctcttt
     3901 tgcatcccct ggagacacgc taccaggcac ggaccttccc aggatggcga caacaccatt
     3961 ttcaaggcct gggccaagga gaccggaaaa tacaccgagg gcgtcgacga agccgaccct
     4021 gccaagtgga aagccaacct cagatgtgcc ctcaacaaga gcagggattt caggctgatt
     4081 tacgacggcc ccagggacat gcctcctcaa ccttacaaaa tctatgaggt gtgcagcaat
     4141 ggccctgccc ccacagacag ccagcctccc gaggactatt ccttcggagc cggagaagag
     4201 gaagaagaag aagaggagct ccagaggatg ctccccagcc tgagcctcac cgaagacgtg
     4261 aagtggcccc ctacactcca gcctcccaca ctgaggcccc ccacactcca accccctaca
     4321 ctgcagcccc ccgtggtgct gggccccccc gcccccgacc ccagccctct ggctcctcct
     4381 cccggcaatc ctgccggctt tagggaactg ctgagcgaag tcctggagcc cggccccctg
     4441 cctgctagcc tgccccctgc tggagagcag ctgctgcctg atctcctcat tagcccccac
     4501 atgctgcccc tcaccgatct ggagatcaag ttccagtaca gaggcaggcc ccccagagcc
     4561 ctgaccatct ccaaccctca cggctgtagg ctcttctact cccagctgga ggccacacaa
     4621 gagcaggtgg aactgttcgg ccccatcagc ctggaacagg tgaggttccc ctcccccgag
     4681 gatatcccct ccgacaagca gaggttttac accaatcagc tcctggatgt gctggacaga
     4741 ggcctgatcc tgcagctgca gggccaggat ctctacgcta tcaggctctg ccagtgcaag
     4801 gtgttttgga gcggaccttg tgctagcgcc cacgactcct gtcccaaccc cattcaaaga
     4861 gaggtgaaga ccaaactctt cagcctcgag cacttcctca atgagctcat cctgttccag
     4921 aaaggccaaa ccaatacccc cccccctttc gagatcttct tctgcttcgg agaggagtgg
     4981 cctgacagga agcccagaga aaaaaagctc attacagtgc aggtcgtgcc cgtcgctgcc
     5041 agactcctcc tggagatgtt tagcggcgag ctcagctgga gcgccgacag catcaggctg
     5101 cagatcagca accccgacct caaagatagg atggtggagc agtttaaaga gctccaccac
     5161 atctggcaat cccagcagag actgcagcct gtcgctcagg ctccccccgg cgccggcctg
     5221 ggcgtgggcc aaggcccctg gcctatgcac cctgctggaa tgcagtgaat cgatcgccca
     5281 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5401 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     5461 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5521 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5581 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5641 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5701 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg acggccgcac
     5761 atgtaaggaa aattttaggg atgttaaaga aaaaaataac acaaaacaaa atataaaaaa
     5821 aatctaacct caagtcaagg cttttctatg gaataaggaa tggacagcag ggggctgttt
     5881 catatactga tgacctcttt atagccaacc tttgttcatg gcagccagca tatgggcata
     5941 tgttgccaaa ctctaaacca aatactcatt ctgatgtttt aaatgatttg ccctcccata
     6001 tgtccttccg agtgagagac acaaaaaatt ccaacacact attgcaatga aaataaattt
     6061 cctttattag ccagaagtca gatgctcaag gggcttcatg atgtccccat aatttttggc
     6121 agagggaaaa agatctcagt ggtatttgtg agccagggca ttggccacac cagtcaccac
     6181 cttctgatag gcagcctgca cctgaggagt gaattcacgc gttttagcct ggcagcatat
     6241 cgagatcgaa gtcatcgaga gcgtcagcag ggagcatatc cagatcaaaa tcatccaggg
     6301 catcggcggg cagcatgtcc aggtcgaagt cgtccagggc gtcggtgggt ccgccgctct
     6361 cgcacttcag ctgcttctcg aggccgcaga tgatcagttc caggccgaac aggaaggcgg
     6421 gctcggcgcc ctgtctgtcg aacagctcga tggcctgctt cagcaggggg ggcatgctgt
     6481 cggtggtggg tgtctctctt tcctctttgg cgacctggtg ttcctgttct tccagcacgc
     6541 agcccagggt gaagtggccc acggcgctca gggcgtacag ggcgttttcc aggctgaagc
     6601 cctgctggca caggaaggcc agctggtttt ccagtgtctc gtactgcttc tcggtgggtc
     6661 tggtgcccag gtgcactttg gcgccgtccc ggtggctcag cagggcgcat ctgtagctct
     6721 tggcgttgtt ccgcaggaag tcctgccagc tctcgccttc cagagggcag ctgtgggtgt
     6781 ggtgccggtc cagcatctcg atgggcaggg cgtccagcag ggcccgcttg ttcttcacgt
     6841 gccagtacag ggtgggctgt tccacgccca gtttctgggc cagcttccgg gtggtcaggc
     6901 cctcgatgcc cacgccgttc agcagttcca gggcgctgtt gatcactttg ctcttgtcca
     6961 gccggctcat gtcgacattc tttgccaaaa tgatgagaca gcacaataac cagcacgttg
     7021 cccaggagct gtaggaaaaa gaagaaggca tgaacatggt tagcagaggc tctagagccg
     7081 ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc
     7141 ccccgcggac agccccgagg ctggagaggg agaaggggac ggcggcgcgg cgacgcacga
     7201 aggccctccc cgcccatttc cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta
     7261 gagggggtgc ggcggcgcct cccagatttc ggctccgcac agatttggga caaaggaagt
     7321 ccctgcgccc tctcgcacga ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga
     7381 cagccgccgg cgctccgggg gccgccgcgc ccctcccccg agccctcccc ggcccgaggc
     7441 ggccccgccc cgcccggcac ccccacctgc cgccaccccc cgcccggcac ggcgagcccc
     7501 gcgccacgcc ccgtacggag ccccgcaccc gaagccgggc cgtgctcagc aactcgggga
     7561 ggggggtgca gggggggttg cagcccgacc gacgcgccca caccccctgc tcaccccccc
     7621 acgcacacac cccgcacgca gcctttgttc ccctcgcagc cccccccgca ccgcggggca
     7681 ccgcccccgg ccgcgctccc ctcgcgcaca ctgcggagcg cacaaagccc cgcgccgcgc
     7741 ccgcagcgct cacagccgcc gggcagcgcg gagccgcacg cggcgctccc cacgcacaca
     7801 cacacgcacg caccccccga gccgctcccc ccgcacaaag ggccctcccg gagcccctca
     7861 aggctttcac gcagccacag aaaagaaaca agccgtcatt aaaccaagcg ctaattacag
     7921 cccggaggag aagggccgtc ccgcccgctc acctgtggga gtaacgcggt cagtcagagc
     7981 cggggcgggc ggcgcgaggc ggcggcggag cggggcacgg ggcgaaggca gcgcgcagcg
     8041 actcccgccc gccgcgcgct tcgcttttta tagggccgcc gccgccgccg cctcgccata
     8101 aaaggaaact ttcggagcgc gccgctctga ttggctgccg ccgcacctct ccgcctcgcc
     8161 ccgccccgcc cctcgccccg ccccgccccg cctggcgcgc gccccccccc cccccccgcc
     8221 cccatcgctg cacaaaataa ttaaaaaata aataaataca aaattggggg tggggagggg
     8281 ggggagatgg ggagagtgaa gcagaacgtg gggctcacct cgaccatggt aatagcgatg
     8341 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     8401 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     8461 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     8521 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     8581 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     8641 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     8701 tgtcgagacc catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct
     8761 cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat
     8821 gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca
     8881 aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg
     8941 ctgatcagcg ggtttataac ttcgtataat gtatgctata cgaagttatg gtaccttact
     9001 cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt
     9061 agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct
     9121 gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct
     9181 tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct
     9241 cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt
     9301 tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg
     9361 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     9421 ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc
     9481 cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca
     9541 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     9601 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga
     9661 tgatggccat gttatcctcc tcgcccttgc tcaccatggt ggcgaccggt agcgctagcg
     9721 taggcgccgg tcacagcttg gatctgtaac ggcgcagaac agaaaacgaa acaaagacgt
     9781 agagttgagc aagcagggtc aggcaaagcg tggagagccg gctgagtcta ggtaggctcc
     9841 aagggagcgc cggacaaagg cccggtctcg acctgagctt taaacttacc tagacggcgg
     9901 acgcagttca ggaggcacca caggcgggag gcggcagaac gcgactcaac cggcgtggat
     9961 ggcggcctca ggtagggcgg cgggcgcgtg aaggagagat gcgagcccct cgaagcttca
    10021 gctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta
    10081 tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc
    10141 agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct ccccccaact
    10201 tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag cgatcgcaga
    10261 tcatgcatcc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc
    10321 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
    10381 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
    10441 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca caggttttta
    10501 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
    10561 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
    10621 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
    10681 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
    10741 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
    10801 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
    10861 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
    10921 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
    10981 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
    11041 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
    11101 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
    11161 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
    11221 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
    11281 ttgttcaatc ctagggccgg gattctcctc cacgtcaccg catgttagaa gacttcctct
    11341 gccctctccg ctgccagatc tctcgaggcc ctgtgggagg aagagaagag gtcagataac
    11401 ttcgtataat gtatgctata cgaagttatg cggccgctgg gagaagggta agagagctca
    11461 atcctcatct tacatagaag gaagtcagta gataatatct tacatttaaa agtcaagaaa
    11521 taacagtctg accatactat ctagaaatat ggaagttaac agtaaggaag ttaaaacagt
    11581 tgaaagtagt ttcgtctaga aagaaagact tggggaagaa caaagaaggc taattttcat
    11641 tatgagcttt attgtaacag aaaagagacc actaactcag ctaggttttc ccaattcctc
    11701 aacactttgg ggcacagtgt ttagtcagag ctgtgatcac tctctgagca catggattgt
    11761 atcttaattt tcatctcatt gaaaatatgt accatttgtc caagtggaaa ctgggctgaa
    11821 gcatgactgt gaccaccacc aaagaaatgg ctagactcgc taaggtctgc tggggtgacc
    11881 cgtgattgtg ttgtgcttga tcttgttgag catggctctt ttaagtcacc atacctgccc
    11941 ctgccaatgc accaaacacc aacttaaagg gactttcagc ttttgtaagt cagtctcagc
    12001 cttacttctg aaccatttct ttgacttctt tcatgactct gatcctgtcc cagttttaag
    12061 cctcccagcc accagtcatg ctgttttgct ctgctattta cattcttctc tacctacttt
    12121 ggacttgatg gtatgcctac tatattagtt atttattgct gtgtaacaaa ccactctgaa
    12181 acatagtggc ttacaaccac aaccatttta ttttattatg aaactgtggg tcagaatgta
    12241 tattggccca ttggcatgat tctcctgcta ttgaaaaagg aagagtatga gtattcaaca
    12301 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
    12361 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
    12421 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
    12481 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
    12541 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
    12601 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
    12661 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
    12721 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
    12781 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
    12841 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
    12901 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
    12961 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
    13021 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    13081 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    13141 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    13201 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    13261 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    13321 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    13381 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    13441 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    13501 gaactctgta gcaccgccta catacctcgc tctgctaatc c
//
