LOCUS       sequence-425633-        4982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteria expression of H2BK120C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Morgan MT, Haj-Yahya M, Ringel AE, Bandi P, Brik A, Wolberger C
  TITLE     Structural basis for histone H2B deubiquitination by the SAGA DUB 
            module.
  JOURNAL   Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681.
  PUBMED    26912860
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "725-8"
COMMENT     Sequence Label: H2BK120C
FEATURES             Location/Qualifiers
     source          1..4982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      58..646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     547..566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     800..817
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    832..971
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(897..916)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1057..1079
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1073..1264)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2722..2741
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2825..2844
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2825..2843
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2875..2897
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(3336..3354)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3350..3397
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(3763..3768,3769..3785,3786..3791))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(3818..3836)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3904..4008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4009..4077,4078..4869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4227..4246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
       61 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
      121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
      181 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
      241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
      301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
      361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
      421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
      481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
      541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
      601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
      661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
      721 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
      781 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
      841 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca
      901 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
      961 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     1021 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     1081 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     1141 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     1201 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     1261 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
     1321 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
     1381 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
     1441 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg
     1501 cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg
     1561 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
     1621 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
     1681 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc
     1741 caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     1801 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     1861 ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
     1921 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat
     1981 ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg
     2041 tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg
     2101 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgtcgccgga
     2161 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
     2221 gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     2281 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
     2341 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
     2401 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
     2461 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     2521 cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     2581 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     2641 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     2701 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     2761 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     2821 aaattaatac gactcactat agggagacca caacggtttc cctctagaaa taattttgtt
     2881 taactttaag aaggagatat atatggccaa gtccgctcca gccccgaaga aaggctccaa
     2941 gaaagcggtg accaagactc agaagaaaga cgggaaaaag cgcaggaaga caaggaagga
     3001 gagttatgcc atttacgtgt acaaggtgct gaagcaggtg caccccgata ccggcatctc
     3061 gtccaaggcc atgagcatca tgaactcctt tgtcaacgat gtgtttgagc gcatcgcagg
     3121 ggaagcctcc cgcctggctc attacaacaa gcgctccacc atcacctccc gggagatcca
     3181 gaccgcggtc cgactgctgc tgcctgggga gttggccaaa cacgccgtgt ccgagggcac
     3241 caaggctgtc acctgttaca ccagcgccaa gtaactgctg ctgccggatc cggctgctaa
     3301 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     3361 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
     3421 atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg
     3481 aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc
     3541 atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt
     3601 cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta
     3661 cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg
     3721 gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga
     3781 taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     3841 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     4261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     4561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4861 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4981 ta
//
