LOCUS       sequence-425667-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, 
            K13A, R15A, K20A, K23A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Uffing A, Gold L, Gensch T, Weiergraber OH, Hoffmann S, Willbold D
  TITLE     Highlighting the hidden: monitoring the avidity-driven association 
            of a fluorescent GABARAP tandem with microtubules in living cells.
  JOURNAL   Autophagy Rep. 2024 May 16;3(1):2348899. doi: 
            10.1080/27694127.2024.2348899. eCollection 2024.
  PUBMED    40395516
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy 
            Rep."; date: "2024-05-16"; volume: "3(1):2348899. doi"; pages: " 
            10.1080/27694127.2024.2348899. eCollection 2024"
COMMENT     Sequence Label: pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(5X)
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..22
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     32..56
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct
       61 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
      121 ggactctagc gtttaaacgc aaccatgaag ttcgtgtaca aagaagagca tccgttcgag
      181 aagcgccgct ctgagggcga gaagatccga aagaaatacc cggaccgggt gccggtgata
      241 gtagaaaagg ctcccaaagc tcggatagga gacctggaca aaaagaaata cctggtgcct
      301 tctgatctca cagttggtca gttctacttc ttgatccgga agcgaattca tctccgagct
      361 gaggatgcct tgtttttctt tgtcaacaat gtcattccac ccaccagtgc cacaatgggt
      421 cagctgtacc aggaacacca tgaagaagac ttctttctct acattgccta cagtgacgaa
      481 agtgtctacg ctctgtccgg atcagatgga tccatggtgt ctaagggcga agagctgatt
      541 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag
      601 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
      661 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc
      721 agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct
      781 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
      841 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
      901 ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag
      961 acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg
     1021 g
//
