LOCUS       sequence-425668-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, 
            K13A, R15A, K20A, K23A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Uffing A, Gold L, Gensch T, Weiergraber OH, Hoffmann S, Willbold D
  TITLE     Highlighting the hidden: monitoring the avidity-driven association 
            of a fluorescent GABARAP tandem with microtubules in living cells.
  JOURNAL   Autophagy Rep. 2024 May 16;3(1):2348899. doi: 
            10.1080/27694127.2024.2348899. eCollection 2024.
  PUBMED    40395516
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy 
            Rep."; date: "2024-05-16"; volume: "3(1):2348899. doi"; pages: " 
            10.1080/27694127.2024.2348899. eCollection 2024"
COMMENT     Sequence Label: pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(5X)
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(355..1047)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDL
                     PSKLGHKLN"
ORIGIN
        1 gtcactgtag gcaatgtaga gaaagaagtc ttcttcatgg tgttcctggt acagctgacc
       61 cattgtggca ctggtgggtg gaatgacatt gttgacaaag aaaaacaagg catcctcagc
      121 tcggagatga attcgcttcc ggatcaagaa gtagaactga ccaactgtga gatcagaagg
      181 caccaggtat ttctttttgt ccaggtctcc tatccgagct ttgggagcct tttctactat
      241 caccggcacc cggtccgggt atttggctcg gattgcctcg ccctcagatg cgcgcgcctc
      301 gaacggatgc tcttctttgt acacgaatgc catagctcga gatctgagtc cggaattaag
      361 cttgtgcccc agtttgctag ggaggtcgca gtatctggcc actgccacct cgtgctgctc
      421 gacgtaggtc tcgttgttgg cctccttgat tctttccagt ctgtagtcca catagtagac
      481 gccaggcatc ttgaggttct tagcgggttt cttggatcta tatgtggtct tggcgtttgc
      541 gatcagatgg ctcccgccca cgagcttcag ggccatgtcg tttctgcctt ccaggccgcc
      601 gtcagcgggg tacagcgtct cggtgaaggc ctcccagccg agtgttttct tctgcatcac
      661 agggccgttg gatgtgaagt tcacccctct gatcttgacg ttgtagatga ggcagccgtc
      721 ctggaggctg gtgtcctggg tagcggtcag cacgcccccg tcttcgtatg tggtgactct
      781 ctcccatgtg aagccctcag ggaaggactg cttgaagaag tcggggatgc cctgggtgtg
      841 gttgatgaag gtcttgctgc cgtagaggaa gctagtagcc aggatgtcga aggcgaaggg
      901 gagagggccg ccctcgacca ccttgattct catggtctgg gtgccctcgt agggcttgcc
      961 ttcgccctcg gatgtgcact tgaagtgatg gttgtccacg gtgccctcca tgtacagctt
     1021 catgtgcatg ttctccttaa tcagctcttc gccc
//
