LOCUS       sequence-42567-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of PSAM-5HT3 HC (L141F,Y115F) neuronal 
            activator, with IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRE...
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..73
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    144..177
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 aattcgccct tttcagtgtt tcgaacctct tcgagggaca taacttcgta taatgtatgc
       61 tatacgaagt tatgggtcga tggtgagatc tggactagag ggtcgatggt gatgcttgga
      121 tcaaaatagg aagaatccca atgataactt cgtataaagt atcctatacg aagttatact
      181 agctagcaag cttgaattca ccatgcgctg ttctccaggc ggcgtgtggc tcgccctggc
      241 tgcttccctt ctgcacgtta gcctgcaggg tgagttccag cgcaaactgt ataaggagct
      301 tgttaagaat tataaccccc tggagcggcc ggtcgcaaat gattcccagc cactgacagt
      361 gtacttcagc ctctccttgc tgcagatcat ggacgtggat gaaaagaacc aggtgctgac
      421 cactaatatt tggttgcaga tgtcctggac cgatcactac ttgcagtgga atgtgagcga
      481 atacccaggt gtaaagactg taagattccc tgacggccaa atctggaaac cagatatcct
      541 gctgttcaac agcgcagacg aaaggtttga tgcaacattt cacaccaacg tgttggtcaa
      601 ttcttcaggc cactgccagt actttccccc tggaatcttc aagtcctcat gctatatcga
      661 cgtccgctgg tttcccttcg acgtccagca ctgcaaactc aaattcggga gctggagcta
      721 c
//
