LOCUS       sequence-42569-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of PSAM-5HT3 HC (L141F,Y115F) neuronal 
            activator, with IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRE...
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(6..39)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(102..135)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(146..165)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          148..280
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ctagtataac ttcgtatagc atacattata cgaagttatg tgtcgatggt gatgcttggc
       61 aattcgggtc gatggtgaag cattggtctt cctattttga tataacttcg tataggatac
      121 tttatacgaa gttatgggga tcatcacctg tggagagaaa ggcaaagtgg atgtcagtaa
      181 gaccaatagg tgcctatcag aaacgcaaga gtcttctctg tctcgacaag cccagtttct
      241 attggtctcc ttaaacctgt cttgtaacct tgatacttac ctgatccaac ggctgcgact
      301 tggggcaggg ggtcctaggg gtggtctggc caggagccgc gggggcggac tgcgcggtgc
      361 ccaggagcac agctgcgctc tcaggcacga cacgactcct ccgctgccca ccgcagactg
      421 aggcagcgct gagtcgccgg cgccgcagcg cagatggtcg cgcccgtgcc cccctatctc
      481 gcgcctcgcg tggtgcggtc cggctgggcc ggcggcggcg cggacgcgac caaggtggcc
      541 gggaagggga gtttgcgggg gaccggcgag tgacgtcagc gcgccttcag tgctgaggcg
      601 gcggtggcgc gcgccgccag gcgggggcga aggcactgtc cgcggtgctg aagctggcag
      661 tgcgcacgcg cctcgccgca tcctgtttcc cctccccctc tc
//
