LOCUS       sequence-42571-e         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal 
            inhibitor, with IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(16..49)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(112..145)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(156..175)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          158..290
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 agtaatctag ctagtataac ttcgtatagc atacattata cgaagttatg tgtcgatggt
       61 gatgcttggc aattcgggtc gatggtgaag cattggtctt cctattttga tataacttcg
      121 tataggatac tttatacgaa gttatgggga tcatcacctg tggagagaaa ggcaaagtgg
      181 atgtcagtaa gaccaatagg tgcctatcag aaacgcaaga gtcttctctg tctcgacaag
      241 cccagtttct attggtctcc ttaaacctgt cttgtaacct tgatacttac ctgatccaac
      301 ggctgcgact tggggcaggg ggtcctaggg gtggtctggc caggagccgc gggggcggac
      361 tgcgcggtgc ccaggagcac agctgcgctc tcaggcacga cacgactcct ccgctgccca
      421 ccgcagactg aggcagcgct gagtcgccgg cgccgcagcg cagatggtcg cgcccgtgcc
      481 cccctatctc gcgcctcgcg tggtgcggtc cggctgggcc ggcggcggcg cggacgcgac
      541 caaggtggcc gggaagggga gtttgcgggg gaccggcgag tgacgtcagc gcgccttcag
      601 tgctgaggcg gcggtggcgc gcgccgccag gcgggggcga aggcactgtc cgcggtgctg
      661 aagc
//
