LOCUS       sequence-42573-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: pBAD-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..94
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             104..805
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(851..868)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cgctttttat cgcaactctc tactgtttct ccatacccgt tttttgggct aacaggagga
       61 attaaccatg gggagccacc atcaccatca ccatggcaga tctatgagcg agctgattaa
      121 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
      181 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
      241 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
      301 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
      361 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
      421 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
      481 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac
      541 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
      601 cgggagccat ctgatcgcaa acgccaagac cacatataga tccaagaaac ccgctaagaa
      661 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
      721 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
      781 caaactgggg cacaagctta attaagaatt cgaagcttgg ctgttttggt ggatgagaga
      841 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
      901 tgcctggcgg cagta
//
