LOCUS       sequence-42574-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: pmTagBFP2-N1
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    32..112
                     /label=MCS
                     /note="multiple cloning site"
     CDS             120..821
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
      121 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
      181 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
      241 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
      301 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
      361 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
      421 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
      481 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
      541 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
      601 ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca
      661 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
      721 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
      781 actgcgacct ccctagcaaa ctggggcaca agcttaatta aagcggccgc gactctagat
      841 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaa
//
