LOCUS       sequence-425761-        9276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant transient expression cassette for GFP (control) under UBQ10 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pDGB3_alpha1-AtUBQ10:GFP-T35S
FEATURES             Location/Qualifiers
     source          1..9276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2008..2010,2011..2013,2014..2727)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2008..2010,2011..2013,2014..2724)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2053..2074)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2314..2333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2661..2682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2941..2965
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4266..4895
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5324..6397
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6463..6657
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6893..6915)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7001..7141
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7056..7075
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7156..7173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7327..7915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7407..7426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8003..8818)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8003..8818)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8726..8745
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9251..9275
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 taacgaattc gtctcaggag agtctagctc aacagagctt ttaacccaaa ttggtacaat
       61 agaatacaac tttagatcat aattctcaaa agaaagagat tccttagcta ttctatctgc
      121 cactccattt ccttctcggc ttgtatgcac aagcataaaa tcctcaaact tgctaagtag
      181 atactttatg tcttggataa ttggattgag acttgacaag cataactttc atgtaaccaa
      241 agacacaagt tgctgagaat ccacctcaaa aatgatcttc ctataattga atcgggataa
      301 tgacagcaca gcccatctaa gagcctccac ttctacttcc agcacgcttc ttacttttac
      361 cacagctctt gcacctaacc ataacacctt ccctgtatga tcgcgaagca cccaccctaa
      421 gccacatttt aatccttctg ttggccatgc cccatcaaag ttgcacttaa cccaagattg
      481 tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta
      541 cattctgagc ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata
      601 cctcattggt tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc
      661 tcccaagtat ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa
      721 gatgttgaac tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt
      781 cttcatagcg aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa
      841 tgaaaaaata ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa
      901 ataagatcca ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg
      961 ccttttttaa cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca
     1021 ataatcatac aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa
     1081 tatgttatac gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt
     1141 gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta
     1201 gaaaatacga aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca
     1261 gctccaccgt atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat
     1321 cgttaaatct caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt
     1381 cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc
     1441 aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg
     1501 tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc
     1561 tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa
     1621 agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc
     1681 aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga
     1741 ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt
     1801 agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt
     1861 ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg
     1921 atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga
     1981 ttaatctgag tttttctgat taacagaatg gtgagcaagg gcgaggagct gttcaccggg
     2041 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2101 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2161 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     2221 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2281 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2341 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2401 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2461 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2521 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2581 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2641 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2701 ctcggcatgg acgagctgta caagtaagct taaatcacca gtctctctct acaaatctat
     2761 ctctctctat aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     2821 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     2881 tctatcaata aaatttctaa ttcctaaaac caaaatccag cgctgtcatg agacgaattc
     2941 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     3001 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     3061 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg
     3121 cttctgacgt tcagtgcagc cgtcatctga aaacgacatg tcgcacaagt cctaagttac
     3181 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat
     3241 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc
     3301 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc
     3361 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg
     3421 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt
     3481 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat
     3541 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc
     3601 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat
     3661 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc
     3721 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg
     3781 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc
     3841 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga
     3901 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca
     3961 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag
     4021 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg
     4081 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg
     4141 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
     4201 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
     4261 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
     4321 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
     4381 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
     4441 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
     4501 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
     4561 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
     4621 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
     4681 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
     4741 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
     4801 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
     4861 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
     4921 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
     4981 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
     5041 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
     5101 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
     5161 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
     5221 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc
     5281 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc
     5341 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
     5401 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
     5461 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
     5521 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
     5581 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
     5641 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
     5701 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc
     5761 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
     5821 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
     5881 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
     5941 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
     6001 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
     6061 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
     6121 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
     6181 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
     6241 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
     6301 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
     6361 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
     6421 aattgcccta gcaggggaaa aaggtcgaaa aggactcttt cctgtggata gcacgtacat
     6481 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
     6541 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
     6601 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
     6661 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc
     6721 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
     6781 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
     6841 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     6901 acatgcagct cccggtgacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6961 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     7021 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     7081 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     7141 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7201 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7261 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7321 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7381 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7441 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7501 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7561 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7621 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7681 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7741 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     7801 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7861 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7981 tttggtcatg cattctaggt gattagaaaa actcatcgag catcaaatga aactgcaatt
     8041 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     8101 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     8161 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     8221 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     8281 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     8341 aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag
     8401 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     8461 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc cctgggatcg
     8521 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     8581 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     8641 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcggtaga
     8701 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     8761 ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata tggctcataa
     8821 cagaacttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
     8881 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
     8941 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
     9001 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
     9061 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
     9121 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
     9181 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
     9241 ggctggctgg tggcaggata tattgtggtg taaaca
//
