LOCUS       sequence-42577-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength maltose sensor constructed from E. coli MalE and
            cpGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Marvin JS, Schreiter ER, Echevarria IM, Looger LL
  TITLE     A genetically encoded, high-signal-to-noise maltose sensor.
  JOURNAL   Proteins. 2011 Nov;79(11):3025-36. doi: 10.1002/prot.23118.
  PUBMED    21989929
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/prot"; 
            journalName: "Proteins"; date: "2011-11"; volume: "79"; issue: "11";
            pages: "3025-36"
COMMENT     Sequence Label: EcMBP165-cpGFP.PPYF.pRSET
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(79..100)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(826..840,841..849))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctcgcccttg ctcaccatgc tccctccggt accgcccttg tacagctcgt ccatgccgag
       61 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      121 gtctttcgaa agtttggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
      181 gtcgccgatg ggggtgttct gctggtagtg gtaggcgagc tgcacgccgc cgtcctcgat
      241 gttgtggcgg atcttgaagt tcgccttgat gccgttcttc tgcttgtcgg ccatgatgaa
      301 gacgttgtag ctgggggggt cagcagcaat cagcggccag gtgaagtacg gttcttgcag
      361 gttgaacatc agcgcgctct tacctttcgc tttcagttct ttatccagcg ccgggatctc
      421 ttcccaggtt tttggcgggt tcggcagcag atctttgtta taaatcagcg ataacgcttc
      481 aacagcgatc gggtaagcaa tcagcttgcc gttgtaacgt acggcatccc aggtaaacgg
      541 atacagcttg tcctggaacg ctttgtccgg ggtgatttca gccaacaggc cagattgagc
      601 gtagccacca aagcggtcgt gtgcccagaa gataatgtca gggccatcgc cagttgccgc
      661 aacctgtggg aatttctctt ccagtttatc cggatgctca acggtgactt taattccggt
      721 atctttctcg aatttcttac cgacttcagc gagaccgtta tagcctttat cgccgttaat
      781 ccagattacc agtttacctt cttcgatttt ggatccccat cgatccttat cgtcatcgtc
      841 gtacagatcc cgacccattt gctgtccacc agtc
//
