LOCUS       sequence-425799-        8915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for PiggyBac mediated integration and stable expression of 
            destabilised EGFP as a reporter to MAPK/ERK pathway activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Zlinska V, Feketova Z, Czyrek A, Chudzian J, Zivkovic ML, Ursachi 
            VC, Dudeja P, Fafilek B, Rynes J, Rico-Llanos G, Koudelka A, Roy T, 
            Biadun M, Raskova V, Svozilova K, Stroblova M, Krzyscik M, Hristova 
            K, Krowarsch D, Foldynova-Trantirkova S, Zakrzewska M, Trantirek L, 
            Krejci P
  TITLE     Specific inhibition of fibroblast growth factor receptor 1 signaling
            by a DNA aptamer.
  JOURNAL   Mol Ther Nucleic Acids. 2024 Nov 28;36(1):102405. doi: 
            10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11.
  PUBMED    39759879
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2024-11-28"; volume: "36(1):102405. doi"; 
            pages: " 10.1016/j.omtn.2024.102405. eCollection 2025 Mar 11"
COMMENT     Sequence Label: pKrox24(MapERK)d1EGFP (KroxDs)
FEATURES             Location/Qualifiers
     source          1..8915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     1421..1440
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        1888..1919
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1946..1955
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1952..1954,1955..1957,1958..2668,2669..2674,
                     2675..2797)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to a mutated version 
                     of residues 422-461 of mouse ornithine decarboxylase, 
                     giving an in vivo half-life of ~1 hour"
                     /label=d1EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1997..2018)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2258..2277)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2605..2626
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4036..4157
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4047..4066)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4101..4120
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4404..4615
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4628..4896
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4934..5308
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(5335..5352)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5341..5565
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6369..6403
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6894..6910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6894..6910)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6907..6929)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6918..6934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6942..6948,6949..6966,6967..6972))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6987..7008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7125..7142)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7296..7884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7376..7395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8055..8846,8847..8915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8678..8697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gtattatgcc cagtacatga
     1381 ccttatggga ctttcctact tggcagtaca tctacgtaaa ctagcaaaat aggctgtccc
     1441 cagtgcaagt gcaggtgcca gaacatttct ctggcctaac tggccggtac ctgagctcgc
     1501 tagcggatgg ggggtgacgt cattcttccg ggcccatatt aggacttccg gcccatatta
     1561 ggccgcccgc gccggatggg gggtgacgtc attcttccgg gcccatatta ggacttccgg
     1621 cccatattag gccgcccgcg ccggatgggg ggtgacgtca ttcttccggg cccatattag
     1681 gacttccggc ccatattagg ccgcccgcgc cggatggggg gtgacgtcat tcttccgggc
     1741 ccatattagg acttccggcc catattaggc cgcccgcgcc ggatgggggg tgacgtcatt
     1801 cttccgggcc catattagga cttccggccc atattaggcc gcccgcgccg atatcaagat
     1861 ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc gacttccagc
     1921 ttggcaatcc ggggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     1981 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2041 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2101 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2161 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2221 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2281 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2341 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2401 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2461 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2521 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2581 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2641 cactctcggc atggacgagc tgtacaagaa gcttagccat ggcttcccgc cggcggtggc
     2701 ggcgcaggat gatggcacgc tgcccatgtc ttgtgcccag gagagcggga tggaccgtca
     2761 ccctgcagcc tgtgcttctg ctaggatcaa tgtgtaggcg gccgcaggga ataaaagaaa
     2821 gcaaagggag aggcaggaaa gacataaaag cacaggaggg aagagatggc cgcaagaggg
     2881 gccacctctt aggtcagatg gaagatctca gagccaagtc cttctactca cgagtagaag
     2941 gaccgttggc caacagccct ttcacttacc atccctgcct cccccgtcct gttccctttg
     3001 acttcagctg cctgaaacag ccatgtccaa gttcttcacc tctatccaaa ggacttgatt
     3061 tgcatggtat tggataaatc atttcagtat cctctccatc acatgcctgg cccttgctcc
     3121 cttcagcgct agaccatcaa gttggcataa agaaaaaaaa atgggtttgg gccctcagaa
     3181 ccctgccctg catctttgta cagcatctgt gccatggatt ttgttttcct tggggtattc
     3241 ttgatgtgaa gataatttgc atactctatt gtattatttg gagttaaatc ctcactttgg
     3301 gggagggggg agcaaagcca agcaaaccaa tgatgatcct ctattttgtg atgactctgc
     3361 tgtgacatta ggtttgaagc attttttttt tcaagcagca gtcctaggta ttaactggag
     3421 catgtgtcag agtgttgttc cgttaatttt gtaaatactg ctcgactgta actctcacat
     3481 gtgacaaagt atggtttgtt tggttgggtt ttgtttttga gaattttttt gcccgtccct
     3541 ttggtttcaa aagtttcacg tcttggtgcc ttttgtgtga cacgccttgc cgatggcttg
     3601 acatgcgcag atgtgaggga cacgctcacc ttagccttaa gggggtagga gtgatgtttt
     3661 gggggaggct ttgagagcaa aaacgaggaa gagggctgag ctgagctttc ggtctccaga
     3721 atgtaagaag aaaaaattta aacaaaaatc tgaactctca aaagtctatt tttctaaact
     3781 gaaaatgtaa atttatacat ctattcagga gttggagtgt tgtggttacc tactgagtag
     3841 gctgcagttt ttgtatgtta tgaacatgaa gttcattatt ttgtggtttt attttacttt
     3901 gtacttgtgt ttgcttaaac aaagtaacct gtttggctta taaacacatt gaatgcgctc
     3961 tattgcccat gggatatgtg gtgtgtatcc ttcagatcta gagtcggggc ggccggccgc
     4021 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     4081 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4141 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4201 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     4261 atccgtttgc gtattgggcg ctcttccgct gatctgcgca gcaccatggc ctgaaataac
     4321 ctctgaaaga ggaacttggt tagctacctt ctaaatcgga tccgcggccg caaggatctg
     4381 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4441 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     4501 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     4561 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     4621 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     4681 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     4741 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     4801 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     4861 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct
     4921 agacgacgcg tccatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     4981 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     5041 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     5101 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     5161 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     5221 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     5281 gcacttcgtg gccgaggagc aggactgact cgagagttta aacccgctga tcagcctcga
     5341 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5401 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5461 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5521 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5581 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5641 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5701 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5761 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5821 ttgattaggg tgatggttca cgtagtgggc cattggtatg gctttttccc cgtatccccc
     5881 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     5941 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
     6001 ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt
     6061 acatccctgg gggctttggg ggggggctgt ccctgatatc tataacaaga aaatatatat
     6121 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa
     6181 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt
     6241 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac
     6301 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt
     6361 caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca
     6421 ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg
     6481 catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt
     6541 tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat
     6601 tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga
     6661 taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag
     6721 caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag
     6781 cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa
     6841 atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag
     6901 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6961 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7021 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7081 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7141 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7201 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7261 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7321 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7381 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7441 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7501 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7561 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7621 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7681 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7741 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7801 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7921 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7981 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8041 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8101 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8161 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8221 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8281 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8341 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8401 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8461 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8521 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8581 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8641 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8701 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8761 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8821 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8881 ggaataaggg cgacacggaa atgttgaata ctcat
//
