LOCUS       sequence-42580-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM
  TITLE     New rabies virus variants for monitoring and manipulating activity 
            and gene expression in defined neural circuits.
  JOURNAL   Neuron. 2011 Aug 25;71(4):617-31.
  PUBMED    21867879
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-08-25"; volume: "71(4)"; pages: "617-31"
COMMENT     Sequence Label: pSADdeltaG-GFP-ER(T2)CreER(T2)
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      210..219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(216..218,219..221,222..734)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIE"
     primer_bind     complement(261..282)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(522..541)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     869..890
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagattata tcccgcaaat ttatcacttg tttacctctg gaggagagaa catatgggct
       61 caactccaac ccttgggagc aatataacaa aaaacatgtt atggtgccat taaaccgctg
      121 catttcatca aagtcaagtt gattaccttt acattttgat cctcttggat gtgaaaaaaa
      181 ctattaacat ccctcaaagg acgggatccg ccaccatggt gagcaagggc gaggagctgt
      241 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      301 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      361 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg
      421 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      481 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      541 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      601 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      661 acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc
      721 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      781 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
      841 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
//
