LOCUS       sequence-425827-       22552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9 guided repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22552)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 22552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: p2301y-tdtomato-dCas9_DegronDead_TPLN188
FEATURES             Location/Qualifiers
     source          1..22552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             496..1125
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1554..2627
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2693..2887
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3123..3145)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3231..3371
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3286..3305
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3386..3403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3557..4145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3637..3656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4401..4808
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4809..5903
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(6983..7777)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8202..8226
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8304..8478
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8535..9332)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8646..8665)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9256..9275
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9395..10071)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(9438..9458)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    10262..10283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(10331..11038)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             11735..11755
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11792..13222
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     16115..16131
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16115..16131
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             16150..16170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(16180..16203,16204..16206,16207..18693,18694..18696,
                     18697..20280)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    21723..21747
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
       61 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
      121 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
      181 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
      241 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
      301 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
      361 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
      421 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
      481 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
      541 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
      601 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
      661 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
      721 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
      781 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
      841 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
      901 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
      961 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     1021 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     1081 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     1141 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     1201 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     1261 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     1321 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     1381 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     1441 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     1501 gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     1561 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     1621 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     1681 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     1741 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     1801 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     1861 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     1921 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     1981 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     2041 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     2101 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     2161 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     2221 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     2281 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     2341 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     2401 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     2461 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     2521 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     2581 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     2641 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     2701 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     2761 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     2821 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     2881 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     2941 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     3001 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     3061 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     3121 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3181 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     3241 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     3301 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     3361 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3961 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4141 ctcaagaaca attccgaccg tcgaggagaa cttctagtat atctacatac ctaatattat
     4201 tgccttatta aaaatggaat cccaacaatt acatcaaaat ccacattctc ttcaaaatca
     4261 attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt tatatttata ggataattat
     4321 actctatttc tcaacaagta attggttgtt tggccgagcg gtctaaggcg cctgattcaa
     4381 gaaatatctt gaccgcagtt aactgtggga atactcaggt atcgtaagat gcaagagttc
     4441 gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca ggggcgctat
     4501 cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc
     4561 atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg
     4621 cttttcatat agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt
     4681 tgacaatatt atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact
     4741 ttctaacttt tcttaccttt tacatttcag caatatatat atatatattt caaggatata
     4801 ccattctaat gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag
     4861 aaatcacagc cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca
     4921 agttcgattt cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgttccac
     4981 ttccagatga ggcgctggaa gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg
     5041 gtggtcctaa atggggaacc ggtagtgtta gacctgaaca aggtttacta aaaatccgta
     5101 aagaacttca attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag
     5161 acttatctcc aatcaagcca caatttgcta aaggtactga cttcgttgtt gtcagagaat
     5221 tagtgggagg tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg
     5281 atagtgaaca atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg
     5341 ccctacaaca tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct
     5401 cttcaagatt atggagaaaa actgtggagg aaaccatcaa gaacgaattt cctacattga
     5461 aggttcaaca tcaattgatt gattctgccg ccatgatcct agttaagaac ccaacccacc
     5521 taaatggtat tataatcacc agcaacatgt ttggtgatat catctccgat gaagcctccg
     5581 ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga
     5641 acaccgcatt tggtttgtac gaaccatgcc acggttctgc tccagatttg ccaaagaata
     5701 aggtcaaccc tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact
     5761 tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa ggttttggat gcaggtatca
     5821 gaactggtga tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag
     5881 aagttaagaa aatccttgct taaaaagatt ctcttttttt atgatatttg tacataaact
     5941 ttataaatga aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg
     6001 gtagacgaaa ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg
     6061 taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt
     6121 gcactttaac attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag
     6181 aaaatcatga aactatgatt cctaatttat atattggagg attttctcta aaaaaaaaaa
     6241 aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta agtcaatacc
     6301 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac
     6361 ggccttaacg acgtagtcga tgacggatcg cttgcctgta acttacacgc gcctcgtatc
     6421 ttttaatgat ggaataattt gggaatttac tctgtgttta tttattttta tgttttgtat
     6481 ttggatttta gaaagtaaat aaagaaggta gaagagttac ggaatgaaga aaaaaaaata
     6541 aacaaaggtt taaaaaattt caacaaaaag cgtactttac atatatattt attagacaag
     6601 aaaagcagat taaatagata tacattcgat taacgataag taaaatgtaa aatcacagga
     6661 ttttcgtgtg tggtcttcta cacagacaag atgaaacaat tcggcattaa tacctgagag
     6721 caggaagagc aagataaaag gtagtatttg ttggcgatcc ccctagagtc ttttacatct
     6781 tcggaaaaca aaaactattt tttctttaat ttcttttttt actttctatt tttaatttat
     6841 atatttatat taaaaaattt aaattataat tatttttata gcacgtcatc tgcgagctcg
     6901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6961 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt
     7021 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
     7081 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
     7141 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
     7201 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
     7261 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
     7321 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
     7381 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
     7441 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
     7501 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
     7561 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
     7621 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
     7681 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
     7741 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
     7801 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
     7861 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
     7921 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
     7981 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
     8041 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
     8101 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
     8161 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     8221 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
     8281 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
     8341 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     8401 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
     8461 cacattatta tggagaaact cgagcttgtc gatcgactct agctagagga tcgatccgaa
     8521 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     8581 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     8641 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     8701 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     8761 tcgccatgtg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     8821 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     8881 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     8941 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     9001 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     9061 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     9121 agccacgata gccgcgctgc ctcgtcctgg agttcattca gggcaccgga caggtcggtc
     9181 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgaa acacggcggc atcagagcag
     9241 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     9301 cctgcgtgca atccatcttg ttcaatcccc atggtcgatc gacagatctg cgaaagctcg
     9361 agagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg
     9421 aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta
     9481 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
     9541 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
     9601 cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt
     9661 ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg
     9721 atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga
     9781 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt
     9841 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
     9901 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
     9961 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
    10021 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
    10081 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
    10141 atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10261 ttaatgtgag ttagctcact cattaggcat ctagtacgcg tcctgcaggc gcgccgaggg
    10321 gatcgagccc ctgctgagcc tcgacatgtt gtcgcaaaat tcgccctgga cccgcccaac
    10381 gatttgtcgt cactgtcaag gtttgacctg cacttcattt ggggcccaca tacaccaaaa
    10441 aaatgctgca taattctcgg ggcagcaagt cggttacccg gccgccgtgc tggaccgggt
    10501 tgaatggtgc ccgtaacttt cggtagagcg gacggccaat actcaacttc aaggaatctc
    10561 acccatgcgc gccggcgggg aaccggagtt cccttcagtg agcgttatta gttcgccgct
    10621 cggtgtgtcg tagatactag cccctggggc acttttgaaa tttgaataag atttatgtaa
    10681 tcagtctttt aggtttgacc ggttctgccg ctttttttaa aattggattt gtaataataa
    10741 aacgcaattg tttgttattg tggcgctcta tcatagatgt cgctataaac ctattcagca
    10801 caatatattg ttttcatttt aatattgtac atataagtag tagggtacaa tcagtaaatt
    10861 gaacggagaa tattattcat aaaaatacga tagtaacggg tgatatattc attagaatga
    10921 accgaaaccg gcggtaagga tctgagctac acatgctcag gttttttaca acgtgcacaa
    10981 cagaattgaa agcaaatatc atgcgatcat aggcgtctcg catatctcat taaagcagat
    11041 tttgtaatat cccgggatat ttcacaaatt gaacatagac tacagaattt tagaaaacaa
    11101 actttctctc tcttatctca cctttatctt ttagagagaa aaagttcgat ttccggttga
    11161 ccggaatgta tctttgtttt ttttgttttg taacatattt cgttttccga tttagatcgg
    11221 atctcctttt ccgttttgtc ggaccttctt ccggtttatc cggatctaat aatatccatc
    11281 ttagacttag ctaagtttgg atctgttttt tggttagctc ttgtcaatcg cctcatcatc
    11341 agcaagaagg tgaaattttt gacaaataaa tcttagaatc atgtagtgtc tttggacctt
    11401 gggaatgata gaaacgattt gttatagcta ctctatgtat cagaccctga ccaagatcca
    11461 acaatctcat aggttttgtg catatgaaac cttcgactaa cgagaagtgg tcttttaatg
    11521 agagagatat ctaaaatgtt atcttaaaag cccactcaaa tctcaaggca taaggtagaa
    11581 atgcaaattt ggaaagtggg ctgggccttt tgtggtaaag gcctgtaacc tagcccaata
    11641 ttagcaaaac cctagacgcg tacattgaca tatataaacc cgcctcctcc ttgtttaggg
    11701 tttctacgtg agagaagacg aaacacaaaa gatgccaaag aagaagagga aagtcggaag
    11761 tggtagtggt agtggaagcg gtagtggcag tatggtgagc aagggcgagg aggtcatcaa
    11821 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
    11881 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
    11941 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
    12001 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
    12061 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
    12121 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
    12181 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
    12241 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
    12301 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
    12361 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
    12421 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac
    12481 cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt
    12541 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
    12601 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
    12661 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
    12721 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
    12781 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
    12841 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
    12901 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
    12961 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
    13021 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
    13081 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
    13141 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
    13201 catggacgag ctgtacaagt aaagagactc ttatcaagaa tcccatctct tgcttgcttt
    13261 tttttgttgt cttccctttg atagggtttg tttttcttgt ttcagtgact ttctatgtta
    13321 aaagataatg tcagtaaaag gatttggttt tctattattc tgaatcgatt acggaagatt
    13381 cttgcttaat tccaatctat acaagtatcg tgaaataatg accgtttatg tgattaggag
    13441 acgtgtttca ttaataaaat ataagatcaa tacattgtta gtagtgataa actatgtaca
    13501 aattgtattg attgtaaaag aaacacaata ggttcctttt ttctacaata tattgtgaca
    13561 gactctctgt tttaacgaat gaattaaatt tgtcaacgaa aaaattaaag aatgatgtga
    13621 tcactttgca ccttggttca tcttcagagg cttctgcatg ttccatgcat tgaggctctc
    13681 tacggtcaca ggttgtgagc cgttgttaaa gacaaacaaa tgtttgcaaa attttccaga
    13741 tcgatttctt cttcctctgt tcttcggcgt tcaatttctg gggttttctc ttcgttttct
    13801 gtaactgaaa cctaaaattt gacctaaaaa aaatctcaaa taatatgatt cagtggtttt
    13861 gtacttttca gttagttgag ttttgcagtt ccgatgagat aaaccaataa ctttgcttag
    13921 atctaattca ttccgttaca cctctgatgg agatggaagg ttcttaataa tgatgccatt
    13981 ttttgggtaa taattttgaa ttagaatcaa gggtataaga ttcataatta acatcactta
    14041 agcaaagttc gtaatatacg accacaggat ataatttttg gtacctcaac aacaaacttt
    14101 ccattcaccc atttatggat acacagtcta gctcaacaga gcttttaacc caaattggta
    14161 caatagaata caactttaga tcataattct caaaagaaag agattcctta gctattctat
    14221 ctgccactcc atttccttct cggcttgtat gcacaagcat aaaatcctca aacttgctaa
    14281 gtagatactt tatgtcttgg ataattggat tgagacttga caagcataac tttcatgtaa
    14341 ccaaagacac aagttgctga gaatccacct caaaaatgat cttcctataa ttgaatcggg
    14401 ataatgacag cacagcccat ctaagagcct ccacttctac ttccagcacg cttcttactt
    14461 ttaccacagc tcttgcacct aaccataaca ccttccctgt atgatcgcga agcacccacc
    14521 ctaagccaca ttttaatcct tctgttggcc atgccccatc aaagttgcac ttaacccaag
    14581 attgtggtgg agcttcccat gtttctcgtc tgtcccgacg gtgttgtggt tggtgctttc
    14641 cttacattct gagcctcttt ccttctaatc cactcatctg catcttcttg tgtccttact
    14701 aatacctcat tggttccaaa ttccctccct ttaagcacca gctcgtttct gttcttccac
    14761 agcctcccaa gtatccaagg gactaaagcc tccacattct tcagatcagg atattcttgt
    14821 ttaagatgtt gaactctatg gaggtttgta tgaactgatg atctaggacc ggataagttc
    14881 ccttcttcat agcgaactta ttcaaagaat gttttgtgta tcattcttgt tacattgtta
    14941 ttaatgaaaa aatattattg gtcattggac tgaacacgag tgttaaatat ggaccaggcc
    15001 ccaaataaga tccattgata tatgaattaa ataacaagaa taaatcgagt caccaaacca
    15061 cttgcctttt ttaacgagac ttgttcacca acttgataca aaagtcatta tcctatgcaa
    15121 atcaataatc atacaaaaat atccaataac actaaaaaat taaaagaaat ggataatttc
    15181 acaatatgtt atacgataaa gaagttactt ttccaagaaa ttcactgatt ttataagccc
    15241 acttgcatta gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat
    15301 tctagaaaat acgaaatacg cttcaatgca gtgggaccca cggttcaatt attgccaatt
    15361 ttcagctcca ccgtatattt aaaaaataaa acgataatgc taaaaaaata taaatcgtaa
    15421 cgatcgttaa atctcaacgg ctggatctta tgacgaccgt tagaaattgt ggttgtcgac
    15481 gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca
    15541 actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt
    15601 tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct
    15661 tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac
    15721 ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc
    15781 tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct
    15841 tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa
    15901 tcttagatcg aagacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta
    15961 gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc
    16021 ttcgatttgt gatttctatc tagatctggt gttagtttct agtttgtgcg atcgaatttg
    16081 tcgattaatc tgagtttttc tgattaacag ggattctaga actagtggat ctacaaaatg
    16141 tctagagccc caaagaagaa gagaaaagtt agacccgggg ataagaaata ctctattggt
    16201 ttggctatcg gtacaaactc tgttggttgg gctgttatta ctgatgaata caaggttcca
    16261 tccaagaagt tcaaggtttt gggtaacact gatagacact ccatcaaaaa gaacttgatt
    16321 ggtgccttgt tgttcgattc tggtgaaact gctgaagcta ctagattgaa aagaaccgct
    16381 agaagaagat acaccagaag aaagaacaga atctgctact tgcaagaaat cttctccaac
    16441 gaaatggcca aggttgatga ttcattcttc cacagattgg aagaatcctt cttggtcgaa
    16501 gaagataaga agcacgaaag acatccaatc ttcggtaaca tcgttgatga agttgcttac
    16561 cacgaaaagt acccaactat ctaccatttg agaaagaagt tggttgactc taccgataag
    16621 gctgatttga gattgatcta tttggctttg gcccacatga ttaagttcag aggtcatttc
    16681 ttgatcgaag gtgatttgaa cccagataac tccgatgttg ataagttgtt catccaatta
    16741 gtccaaacct acaatcaatt attcgaagaa aacccaatca acgcctctgg tgttgatgct
    16801 aaagctattt tgtctgccag attgtccaag tccagaagat tagaaaattt gatcgcccaa
    16861 ttaccaggtg aaaagaagaa tggtttgttc ggtaatttga ttgccttgtc tttgggtttg
    16921 actccaaact tcaagtccaa tttcgatttg gctgaagatg ccaagttgca attatctaag
    16981 gatacctacg atgacgattt ggataacttg ttggctcaaa tcggtgatca atacgctgat
    17041 ttgtttttgg ctgctaagaa cttgtccgat gccattttgt tgtccgatat tttgagagtc
    17101 aacaccgaaa ttactaaggc tccattgtct gcctctatga tcaaaagata cgatgaacac
    17161 caccaagact tgactttgtt gaaggctttg gtcagacaac aattacctga aaagtacaaa
    17221 gaaattttct tcgatcaatc caagaacggt tacgccggtt atattgatgg tggtgcttct
    17281 caagaagaat tttacaagtt catcaagcca atcttggaaa agatggacgg tactgaagaa
    17341 ttattggtca agttgaacag agaagatttg ttgagaaagc aaagaacctt cgacaacggt
    17401 tctattccac atcaaattca cttgggtgaa ttgcacgcaa ttttgagaag acaagaagat
    17461 ttttatccat tcttgaagga caacagagaa aagatcgaaa agattctgac cttcagaatc
    17521 ccttactacg ttggtccatt ggctagaggt aattcaagat ttgcctggat gactagaaag
    17581 tccgaagaaa ctattactcc ttggaacttc gaagaagttg tagataaggg tgcttctgcc
    17641 caatccttta ttgaaagaat gaccaacttc gacaagaact tgccaaacga aaaggttttg
    17701 ccaaagcact ctttgttgta cgaatacttc accgtctaca acgaattgac taaggttaag
    17761 tacgtcaccg aaggtatgag aaaaccagct tttttatccg gtgaacaaaa gaaggctatc
    17821 gtcgatttgt tgttcaagac caacagaaag gttactgtca agcaattaaa agaagattac
    17881 ttcaagaaaa tcgaatgctt cgactccgtt gaaatttctg gtgtcgaaga tagattcaat
    17941 gcctctttag gtacttacca tgacttgttg aaaatcatca aggacaagga tttcttggac
    18001 aacgaagaaa acgaagatat tttggaagat attgtcttga cattgacctt gtttgaagat
    18061 agagaaatga ttgaagaaag attgaaaacc tacgcccact tgttcgatga taaggttatg
    18121 aagcaattaa agagaagaag atacactggt tggggtagat tgtccagaaa attgattaac
    18181 ggtatcagag acaagcaatc cggtaagacc attttggact ttttgaagtc tgatggtttc
    18241 gctaacagaa acttcatgca attaatccac gacgattcct tgactttcaa agaagatata
    18301 caaaaggccc aagtctctgg tcaaggtgat tctttacatg aacatatcgc taacttggct
    18361 ggttctccag ctattaagaa gggtatttta caaaccgtta aggtcgttga cgaattggtc
    18421 aaagttatgg gtagacataa gccagaaaac atcgttatcg aaatggctag agaaaatcaa
    18481 accacccaaa agggtcaaaa gaactccaga gaaagaatga agagaatcga agaaggtatc
    18541 aaagaattgg gttcccaaat tttgaaagaa cacccagttg aaaacaccca attacaaaac
    18601 gaaaagttgt acttgtacta cttgcaaaac ggtagagata tgtacgttga ccaagaattg
    18661 gacatcaaca gattgtctga ttacgatgtt gacgctatcg ttccacaatc ttttttgaag
    18721 gatgactcca ttgacaacaa ggtcttgact agatccgata agaatagagg taagtccgat
    18781 aacgttccat ctgaagaagt cgttaagaaa atgaagaact attggagaca attattgaac
    18841 gccaagttga tcacccaaag aaagtttgac aatttgacca aggctgaaag aggtggtttg
    18901 tctgaattgg ataaggcagg tttcatcaaa agacaattag tagaaaccag acaaatcacc
    18961 aagcacgttg ctcaaatttt ggatagtaga atgaacacta agtacgacga aaacgacaaa
    19021 ttgatcagag aagttaaggt cattaccttg aagtccaagt tggtttccga tttcagaaag
    19081 gacttccaat tctacaaggt cagagaaatc aacaactacc atcatgcaca tgatgcttac
    19141 ttgaatgctg ttgttggtac tgccttgatt aagaagtatc caaagttgga atccgaattt
    19201 gtctacggtg attacaaggt ttacgacgtt agaaagatga tcgccaagtc cgaacaagaa
    19261 attggtaaag ctactgccaa atacttcttc tactccaata ttatgaattt ctttaagacc
    19321 gaaatcactt tggccaacgg tgaaattaga aaaagaccat tgattgaaac taatggtgaa
    19381 acaggtgaaa tcgtttggga taagggtaga gattttgcca ctgttagaaa ggtattgtcc
    19441 atgccacaag taaacatcgt caaaaagacc gaagttcaaa ctggtggttt ctccaaagaa
    19501 tccattttgc ctaagagaaa ctccgataag ttgatcgcta gaaaaaaaga ctgggaccca
    19561 aaaaagtacg gtggttttga ttctccaact gttgcttact ctgttttggt tgttgctaag
    19621 gtcgaaaagg gtaagagtaa gaagttgaag tccgtcaaag aattattagg tatcactatc
    19681 atggaaagat cctcattcga aaagaatcct atcgactttt tggaagccaa gggttacaaa
    19741 gaagtcaaga aggacttgat cattaagttg ccaaagtaca gtttgttcga attggaaaat
    19801 ggtagaaaga gaatgttggc ttctgccggt gaattacaaa agggtaatga attggctttg
    19861 ccatccaagt acgttaattt cttatacttg gcctcccact acgaaaaatt gaaaggttct
    19921 cctgaagata acgaacaaaa gcaattattt gtcgaacaac acaagcacta cttggacgaa
    19981 atcattgaac aaatttccga attttccaaa agagtcattt tggctgacgc caatttggac
    20041 aaagttttgt cagcttacaa caagcacaga gataagccaa ttagagaaca agctgaaaac
    20101 atcattcact tgttcacttt gactaacttg ggtgctccag ctgcttttaa gtatttcgat
    20161 accactatcg acagaaagag atacacctct accaaagaag ttttggacgc tactttgatc
    20221 caccaatcta ttactggttt gtacgaaact agaatcgact tgtctcaatt aggtggtgat
    20281 ggttctggat caggtagtgg ctcaggatct atggaagaag aaaagagatt ggagctaagg
    20341 ctagctcctc cttgtcacca attcacttcc aacaacaaca tcaatggatc taaacaaaaa
    20401 agctcgacca aagaaacatc attcctttcc aataacaggg ttgaggtagc tccagtggtg
    20461 ggatggccgc cggggagatc atcccggaga aacctaacgg cacaactaaa ggaggagatg
    20521 aagaagaagg agagtgatga agagaaggaa ttgtacgtta agatcaacat ggaaggagtt
    20581 ccaataggaa gaaaagtcaa cctttcagct tataacaact accaacagct ttcacatgcc
    20641 gttgaccaac tcttctctaa gaaagattcg tgggatctaa acagacaata cactttggtc
    20701 tacgaagaca ctgaaggaga taaagttctg gtcggggatg ttccttggga gatgtttgta
    20761 tctactgtaa agaggttgca tgttttaaag acctcccacg ccttctcact ctcacctaga
    20821 aaacatggca aggaagctgc aggaggcggt ggaagcatgt cttctcttag tagagagctc
    20881 gttttcttga tcttacagtt tctcgatgaa gagaagttta aagagactgt tcacaagctt
    20941 gaacaagaat ctgggttttt cttcaatatg aagtattttg aggatgaggt tcacaatgga
    21001 aattgggatg aggttgagaa gtatctctct ggtttcacta aagtagatga taatagatac
    21061 tctatgaaga tattcttcga gataagaaag cagaagtatc tcgaggcttt ggataagcat
    21121 gatcgtccca aggctgtgga tatactagtg aaggatttga aagtgttttc aacttttaat
    21181 gaggagcttt tcaaggaaat aacacagctc ttgacattgg agaacttccg ggagaatgaa
    21241 cagttatcca agtatgggga caccaagtct gcaagagcga tcatgttggt ggaactcaaa
    21301 aagctgattg aggcgaatcc tttattccgt gataaattgc agttccctac tcttagaaat
    21361 tcaaggctga ggactttgat caaccagagc ttaaattggc aacaccagct ttgtaaaaat
    21421 tagcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
    21481 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
    21541 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
    21601 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
    21661 atgttactag atcgggcctg caggacgcgt ggcgcgccag atccaattaa actatcagtg
    21721 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
    21781 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
    21841 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc
    21901 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
    21961 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
    22021 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
    22081 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
    22141 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
    22201 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
    22261 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
    22321 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
    22381 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
    22441 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
    22501 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cg
//
