LOCUS       sequence-425849-        5711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human CORO1A. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_CORO1A:1-461
FEATURES             Location/Qualifiers
     source          1..5711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2774..2895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2785..2804)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3010..3043)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3053..3100
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3132..3926
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3186..3205)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3796..3815
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4084..4672)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4164..4183)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4798..4846
                     /note="synthetic polyadenylation signal"
     CDS             complement(4870..5268)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5265..5274
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5299..5656)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5313..5448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5465..5656
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5613..5633
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgagccg gcaggtggtc
     1261 cgctccagca agttccgcca cgtgtttgga cagccggcca aggccgacca gtgctatgaa
     1321 gatgtgcgcg tctcacagac cacctgggac agtggcttct gtgctgtcaa ccctaagttt
     1381 gtggccctga tctgtgaggc cagcggggga ggggccttcc tggtgctgcc cctgggcaag
     1441 actggacgtg tggacaagaa tgcgcccacg gtctgtggcc acacagcccc tgtgctagac
     1501 atcgcctggt gcccgcacaa tgacaacgtc attgccagtg gctccgagga ctgcacagtc
     1561 atggtgtggg agatcccgga tgggggcctg atgctgcccc tgcgggagcc cgtcgtcacc
     1621 ctggagggcc acaccaagcg tgtgggcatt gtggcctggc acaccacagc ccagaacgtg
     1681 ctgctcagtg caggttgtga caacgtgatc atggtgtggg acgtgggcac tggggcggcc
     1741 atgctgacac tgggcccaga ggtgcaccca gacacgatct acagtgtgga ctggagccga
     1801 gatggaggcc tcatttgtac ctcctgccgt gacaagcgcg tgcgcatcat cgagccccgc
     1861 aaaggcactg tcgtagctga gaaggaccgt ccccacgagg ggacccggcc cgtgcgtgca
     1921 gtgttcgtgt cggaggggaa gatcctgacc acgggcttca gccgcatgag tgagcggcag
     1981 gtggcgctgt gggacacaaa gcacctggag gagccgctgt ccctgcagga gctggacacc
     2041 agcagcggtg tcctgctgcc cttctttgac cctgacacca acatcgtcta cctctgtggc
     2101 aagggtgaca gctcaatccg gtactttgag atcacttccg aggccccttt cctgcactat
     2161 ctctccatgt tcagttccaa ggagtcccag cggggcatgg gctacatgcc caaacgtggc
     2221 ctggaggtga acaagtgtga gatcgccagg ttctacaagc tgcacgagcg gaggtgtgag
     2281 cccattgcca tgacagtgcc tcgaaagtcg gacctgttcc aggaggacct gtacccaccc
     2341 accgcagggc ccgaccctgc cctcacggct gaggagtggc tggggggtcg ggatgctggg
     2401 cccctcctca tctccctcaa ggatggctac gtacccccaa agagccggga gctgagggtc
     2461 aaccggggcc tggacaccgg gcgcaggagg gcagcaccag aggccagtgg cactcccagc
     2521 tcggatgccg tgtctcggct ggaggaggag atgcggaagc tccaggccac ggtgcaggag
     2581 ctccagaagc gcttggacag gctggaggag acagtccagg ccaagggctc gagcggtggg
     2641 agctccggtg tgagcggctg gcggctgttc aagaagatta gctaagttta aacggccgcg
     2701 actctagact gcaggcatgc aagctgattt ggctgctgcc accgctgagc aaggccgctt
     2761 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     2821 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     2881 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     2941 atgtgggagg tttttttaag caagtaaaac ctctacaaat gtggtaaaat cttaattaag
     3001 acttctaata taacttcgta tagcatacat tatacgaagt tatggttcca ctgttgacaa
     3061 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccca
     3121 ggaggcagat catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     3181 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3241 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3301 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3361 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3421 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3481 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     3541 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     3601 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     3661 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     3721 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     3781 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     3841 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     3901 atcgccttct tgacgagttc ttctgattcg aaatgaccga ccaagcgacg cccaaccggt
     3961 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4021 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4081 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4141 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4201 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4261 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4321 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4381 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4441 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4501 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4561 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4621 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatttc aagaagatcc
     4681 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4741 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttatagtccg gaatcgtcac
     4801 acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg ccatcgtgat
     4861 ggctagagtt tagccctccc acacataacc agagggcagc aattcacgaa tcccaactgc
     4921 cgtcggctgt ccatcactgt ccttcactat ggctttgatc ccaggatgca gatcgagaag
     4981 cacctgtcgg caccgtccgc aggggctcaa gatgcccctg ttctcatttc cgatcgcgac
     5041 gatacaagtc aggttgccag ctgccgcagc agcagcagtg cccagcacca cgagttctgc
     5101 acaaggtccc ccagtaaaat gatatacatt gacaccagtg aagatgcggc cgtcgctaga
     5161 gagagctgcg ctggcgacgc tgtagtcttc agagatgggg atgctgttga ttgtagccgt
     5221 tgctctttca atgagggtgg attcttcttg agacaaaggc ttggccatgg tggcgctagt
     5281 gtcagaagaa tcgagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     5341 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     5401 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     5461 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5521 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     5581 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     5641 gttctttccg cctcagaagg tacctaacca agttcctctt tcagaggtta tttcaggcca
     5701 tggtgctgcg c
//
