LOCUS       sequence-425854-        5867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human SMU1. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_SMU1:1-513
FEATURES             Location/Qualifiers
     source          1..5867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2930..3051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2941..2960)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3166..3199)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3209..3256
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3288..4082
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3342..3361)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3952..3971
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4240..4828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4320..4339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4954..5002
                     /note="synthetic polyadenylation signal"
     CDS             complement(5026..5424)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5421..5430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5455..5812)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5469..5604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5621..5812
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5769..5789
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgtcgat cgaaatcgaa
     1261 tcttcggatg tgatccgcct tattatgcag tacttgaagg agaacagttt acatcgggcg
     1321 ttagccacct tgcaggagga gactactgtg tctctgaata ctgtggacag cattgagagt
     1381 tttgtggctg acattaacag tggccattgg gatactgtgt tgcaggctat acagtctctg
     1441 aaattgccag acaaaaccct cattgacctc tatgaacagg ttgttctgga attgatagag
     1501 ctccgtgaat tgggtgctgc caggtcactt ttgagacaga ctgatcccat gatcatgtta
     1561 aaacaaacac agccagagcg atatattcat ctggagaacc ttttggccag gtcttacttt
     1621 gatcctcgtg aggcataccc agatggaagt agcaaagaaa agagaagagc agcaattgcc
     1681 caggccttag ctggcgaagt cagtgtggtg cctccatctc gtctcatggc attgctggga
     1741 caggcactga agtggcagca gcatcaggga ttgcttcctc ctggtatgac catagatttg
     1801 tttcgaggca aggcagctgt caaagatgtg gaagaagaaa agtttcctac acaactgagc
     1861 aggcatatta agtttggtca gaaatcacat gtggagtgtg ctcgattttc tccagatggt
     1921 cagtatttgg tcactgggtc tgttgatgga ttcattgaag tatggaactt tactactgga
     1981 aaaatcagaa aggatcttaa gtaccaggcc caagataact ttatgatgat ggatgatgct
     2041 gtcctctgca tgtgtttcag cagagataca gaaatgttag caactggggc ccaagatgga
     2101 aaaatcaagg tgtggaagat tcagagtgga caatgtttaa ggagatttga gagggcacac
     2161 agtaagggtg tcacctgtct aagcttttct aaggatagca gtcagatcct tagtgcttct
     2221 tttgaccaga caattagaat tcatggttta aaatctggga aaaccctgaa ggaatttcgt
     2281 ggccattcct cctttgttaa cgaagcaaca tttacacaag atggacatta cattattagt
     2341 gcatcctctg atggcactgt aaagatctgg aatatgaaga ccacagaatg ttcaaatacc
     2401 tttaaatccc tgggcagcac cgcagggaca gatattaccg tcaacagtgt gattctactt
     2461 cctaaaaacc ctgagcactt tgtggtgtgc aacagatcaa acacggtggt catcatgaac
     2521 atgcaggggc agattgtcag aagcttcagt tctggtaaaa gagaaggtgg ggactttgtt
     2581 tgctgtgccc tctctccccg tggtgaatgg atctactgtg taggggagga ctttgtgctc
     2641 tactgtttca gtacagtcac tggcaaactg gagagaactt tgacagtgca cgagaaggat
     2701 gtgattggta ttgcacatca ccctcatcag aacctgattg ctacctacag tgaagatgga
     2761 ctcctaaagc tctggaaacc aggctcgagc ggtgggagct ccggtgtgag cggctggcgg
     2821 ctgttcaaga agattagcta agtttaaacg gccgcgactc tagactgcag gcatgcaagc
     2881 tgatttggct gctgccaccg ctgagcaagg ccgcttcgag cagacatgat aagatacatt
     2941 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3001 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3061 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag
     3121 taaaacctct acaaatgtgg taaaatctta attaagactt ctaatataac ttcgtatagc
     3181 atacattata cgaagttatg gttccactgt tgacaattaa tcatcggcat agtatatcgg
     3241 catagtataa tacgacaagg tgaggaacta aacccaggag gcagatcatg attgaacaag
     3301 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3361 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3421 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     3481 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3541 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3601 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3661 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3721 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3781 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     3841 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3901 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     3961 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4021 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4081 gattcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa aggcggtaat
     4141 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4201 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4261 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4321 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4381 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4441 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4501 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4561 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4621 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4681 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4741 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4801 gattacgcgc agaaaaaaag gatttcaaga agatcctttg atcttttcta cggggtctga
     4861 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4921 cttcacctag atccttttat agtccggaat cgtcacacaa aaaaccaaca cacagatgta
     4981 atgaaaataa agatatttta ttgcggccat cgtgatggct agagtttagc cctcccacac
     5041 ataaccagag ggcagcaatt cacgaatccc aactgccgtc ggctgtccat cactgtcctt
     5101 cactatggct ttgatcccag gatgcagatc gagaagcacc tgtcggcacc gtccgcaggg
     5161 gctcaagatg cccctgttct catttccgat cgcgacgata caagtcaggt tgccagctgc
     5221 cgcagcagca gcagtgccca gcaccacgag ttctgcacaa ggtcccccag taaaatgata
     5281 tacattgaca ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg cgacgctgta
     5341 gtcttcagag atggggatgc tgttgattgt agccgttgct ctttcaatga gggtggattc
     5401 ttcttgagac aaaggcttgg ccatggtggc gctagtgtca gaagaatcga gctttttgca
     5461 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     5521 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     5581 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     5641 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     5701 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     5761 ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc agaaggtacc
     5821 taaccaagtt cctctttcag aggttatttc aggccatggt gctgcgc
//
