LOCUS       sequence-425855-        6521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human COP1. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_COP1:1-731
FEATURES             Location/Qualifiers
     source          1..6521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3584..3705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3595..3614)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3820..3853)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3863..3910
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3942..4736
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3996..4015)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4606..4625
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4894..5482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4974..4993)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5608..5656
                     /note="synthetic polyadenylation signal"
     CDS             complement(5680..6078)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6075..6084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6109..6466)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6123..6258
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6275..6466
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6423..6443
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgtctgg tagccgccag
     1261 gccgggtcgg gctccgctgg gacaagcccc gggtcctcgg cggcctcctc ggtgacttcc
     1321 gcctcctcgt ctttatcctc ttccccgtcg ccgccttccg tggcggtttc ggcggcagcg
     1381 ctggtgtccg gcggggtggc ccaggccgcc ggctcgggcg gcctcggggg cccggtgcgg
     1441 cctgtgttgg tggcgcccgc cgtatcgggt agcggcggcg gggcggtgtc cacgggcctg
     1501 tcccggcaca gctgcgcggc caggcccagc gccggcgtag gaggcagcag ctccagccta
     1561 ggcagcggca gcaggaagcg acctctcctc gcccccctct gcaacgggct catcaactcc
     1621 tacgaggaca aaagcaacga cttcgtatgc cccatctgct ttgatatgat tgaagaagca
     1681 tacatgacaa aatgtggcca cagcttttgc tacaagtgta ttcatcagag tttggaggac
     1741 aataatagat gtcccaagtg taactatgtt gtggacaata ttgaccatct gtatcctaat
     1801 ttcttggtga atgaactcat tcttaaacag aagcaaagat ttgaggaaaa gaggttcaaa
     1861 ttggaccact cagtgagtag caccaatggc cacaggtggc agatatttca agattggttg
     1921 ggaactgacc aagataacct tgatttggcc aatgtcaatc ttatgttgga gttactagtg
     1981 cagaagaaga aacaactgga agcagaatca catgcagccc aactacagat tcttatggaa
     2041 ttcctcaagg ttgcaagaag aaataagaga gagcaactgg aacagatcca gaaggagcta
     2101 agtgttttgg aagaggatat taagagagtg gaagaaatga gtggcttata ctctcctgtc
     2161 agtgaggata gcacagtgcc tcaatttgaa gctccttctc catcacacag tagtattatt
     2221 gattccacag aatacagcca acctccaggt ttcagtggca gttctcagac aaagaaacag
     2281 ccttggtata atagcacgtt agcatcaaga cgaaaacgac ttactgctca ttttgaagac
     2341 ttggagcagt gttacttttc tacaaggatg tctcgtatct cagatgacag tcgaactgca
     2401 agccagttgg atgaatttca ggaatgcttg tccaagttta ctcgatataa ttcagtacga
     2461 cctttagcca cattgtcata tgctagtgat ctctataatg gttccagtat agtctctagt
     2521 attgaatttg accgggattg tgactatttt gcgattgctg gagttacaaa gaagattaaa
     2581 gtctatgaat atgacactgt catccaggat gcagtggata ttcattaccc tgagaatgaa
     2641 atgacctgca attcgaaaat cagctgtatc agttggagta gttaccataa gaacctgtta
     2701 gctagcagtg attatgaagg cactgttatt ttatgggatg gattcacagg acagaggtca
     2761 aaggtctatc aggagcatga gaagaggtgt tggagtgttg actttaattt gatggatcct
     2821 aaactcttgg cttcaggttc tgatgatgca aaagtgaagc tgtggtctac caatctagac
     2881 aactcagtgg caagcattga ggcaaaggct aatgtgtgct gtgttaaatt cagcccctct
     2941 tccagatacc atttggcttt cggctgtgca gatcactgtg tccactacta tgatcttcgt
     3001 aacactaaac agccaatcat ggtattcaaa ggacaccgta aagcagtctc ttatgcaaag
     3061 tttgtgagtg gtgaggaaat tgtctctgcc tcaacagaca gtcagctaaa actgtggaat
     3121 gtagggaaac catactgcct acgttccttc aagggtcata tcaatgaaaa aaactttgta
     3181 ggcctggctt ccaatggaga ttatatagct tgtggaagtg aaaataactc tctctacctg
     3241 tactataaag gactttctaa gactttgcta acttttaagt ttgatacagt caaaagtgtt
     3301 ctcgacaaag accgaaaaga agatgataca aatgaatttg ttagtgctgt gtgctggagg
     3361 gcactaccag atggggagtc caatgtgctg attgctgcta acagtcaggg tacaattaag
     3421 gtgctagaat tggtaggctc gagcggtggg agctccggtg tgagcggctg gcggctgttc
     3481 aagaagatta gctaagttta aacggccgcg actctagact gcaggcatgc aagctgattt
     3541 ggctgctgcc accgctgagc aaggccgctt cgagcagaca tgataagata cattgatgag
     3601 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3661 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     3721 attcatttta tgtttcaggt tcagggggag atgtgggagg tttttttaag caagtaaaac
     3781 ctctacaaat gtggtaaaat cttaattaag acttctaata taacttcgta tagcatacat
     3841 tatacgaagt tatggttcca ctgttgacaa ttaatcatcg gcatagtata tcggcatagt
     3901 ataatacgac aaggtgagga actaaaccca ggaggcagat catgattgaa caagatggat
     3961 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4021 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4081 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     4141 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4201 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4261 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4321 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4381 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4441 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     4501 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4561 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4621 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4681 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgattcg
     4741 aaatgaccga ccaagcgacg cccaaccggt atcagctcac tcaaaggcgg taatacggtt
     4801 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4861 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4921 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4981 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5041 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5101 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5161 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5221 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5281 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     5341 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5401 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5461 gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5521 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5581 ctagatcctt ttatagtccg gaatcgtcac acaaaaaacc aacacacaga tgtaatgaaa
     5641 ataaagatat tttattgcgg ccatcgtgat ggctagagtt tagccctccc acacataacc
     5701 agagggcagc aattcacgaa tcccaactgc cgtcggctgt ccatcactgt ccttcactat
     5761 ggctttgatc ccaggatgca gatcgagaag cacctgtcgg caccgtccgc aggggctcaa
     5821 gatgcccctg ttctcatttc cgatcgcgac gatacaagtc aggttgccag ctgccgcagc
     5881 agcagcagtg cccagcacca cgagttctgc acaaggtccc ccagtaaaat gatatacatt
     5941 gacaccagtg aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc tgtagtcttc
     6001 agagatgggg atgctgttga ttgtagccgt tgctctttca atgagggtgg attcttcttg
     6061 agacaaaggc ttggccatgg tggcgctagt gtcagaagaa tcgagctttt tgcaaaagcc
     6121 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     6181 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     6241 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     6301 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     6361 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     6421 acaccctaac tgacacacat tccacagctg gttctttccg cctcagaagg tacctaacca
     6481 agttcctctt tcagaggtta tttcaggcca tggtgctgcg c
//
