LOCUS       sequence-425859-        7886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human PALB2. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_PALB2:1-1186
FEATURES             Location/Qualifiers
     source          1..7886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4949..5070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4960..4979)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(5185..5218)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5228..5275
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5307..6101
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5361..5380)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5971..5990
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(6259..6847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6339..6358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6973..7021
                     /note="synthetic polyadenylation signal"
     CDS             complement(7045..7443)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      7440..7449
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7474..7831)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7488..7623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7640..7831
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7788..7808
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggacga gcctcccggg
     1261 aagcccctca gctgtgagga gaaggaaaag ttaaaggaga aattagcatt cttgaaaagg
     1321 gaatacagca agacactagc ccgccttcag cgtgcccaaa gagctgaaaa gattaagcat
     1381 tctattaaga aaacagtaga agaacaagat tgtttgtctc agcaggatct ctcaccgcag
     1441 ctaaaacact cagaacctaa aaataaaata tgtgtttatg acaagttaca catcaaaacc
     1501 catcttgatg aagaaactgg agaaaagaca tctatcacac ttgatgttgg gcctgagtcc
     1561 tttaaccctg gagatggccc aggaggatta cctatacaaa gaacagatga cacccaagaa
     1621 cattttcccc acagggtcag tgaccctagt ggtgagcaaa agcagaagct gccaagcaga
     1681 agaaagaagc agcagaagag gacatttatt tcacaggaga gagactgtgt ctttggcact
     1741 gattcactca gattgtctgg gaaaagacta aaggaacagg aagaaatcag tagcaaaaat
     1801 cctgctagat caccagtaac tgaaataaga actcaccttt taagtcttaa atctgaactt
     1861 ccagattctc cagaaccagt tacagaaatt aatgaagaca gtgtattaat tccaccaact
     1921 gcccaaccag aaaaaggtgt tgatacattc ctaagaagac ctaatttcac cagggcgact
     1981 acagttcctt tacagactct atcagatagc ggtagtagtc agcaccttga acacattcct
     2041 cctaaaggta gcagtgaact tactactcac gacctaaaaa acattagatt tacttcacct
     2101 gtaagtttgg aggcacaagg caaaaaaatg actgtctcta cagataacct ccttgtaaat
     2161 aaagctataa gtaaaagtgg ccaactgccc acaagttcta atttagaggc aaatatttca
     2221 tgttctctaa atgaactcac ctacaataac ttaccagcaa atgaaaacca aaacttaaaa
     2281 gaacaaaatc aaacagagaa atctttaaaa tctcccagtg acactcttga tggcaggaat
     2341 gaaaatcttc aggaaagtga gattctaagt caacctaaga gtcttagcct ggaagcaacc
     2401 tctcctcttt ctgcagaaaa acattcttgc acagtgcctg aaggccttct gtttcctgca
     2461 gaatattatg ttagaacaac acgaagcatg tccaattgcc agaggaaagt agccgtggag
     2521 gctgtcattc agagtcattt ggatgtcaag aaaaaagggt ttaaaaataa aaataaggat
     2581 gcaagtaaaa atttaaacct ttccaatgag gaaactgacc aaagtgaaat taggatgtct
     2641 ggcacatgca caggacaacc aagttcaaga acctctcaga aacttctctc attaactaaa
     2701 gtcagctctc ccgctgggcc cactgaagat aatgacttgt ctaggaaggc agttgcccaa
     2761 gcacctggta gaagatacac aggaaaaaga aaatcagcct gcaccccagc atcagatcat
     2821 tgtgaaccac ttttgccaac ttctagcctg tcgattgtta acaggtccaa ggaagaagtc
     2881 acctcacaca aatatcagca cgaaaaatta tttattcaag tgaaagggaa gaaaagtcgt
     2941 catcaaaaag aggattccct ttcttggagt aatagtgctt atttatcctt ggatgatgat
     3001 gctttcacgg ctccatttca tagggatgga atgctgagtt taaagcaact actgtctttt
     3061 ctcagtatca cagactttca gttacctgat gaagactttg gacctcttaa gcttgaaaaa
     3121 gtgaagtcct gctcagaaaa accagtggag ccctttgagt caaaaatgtt tggagagaga
     3181 catcttaaag agggaagctg tatttttcca gaggaactga gtcctaaacg catggataca
     3241 gaaatggagg acttagaaga ggaccttatt gttctaccag gaaaatcaca tcccaaaagg
     3301 ccaaactcgc aaagccagca tacaaagacg ggcctttctt catccatatt actttatact
     3361 cctttaaata cggttgcgcc tgatgataat gacaggccta ccacagacat gtgttcacct
     3421 gctttcccca tcttaggtac tactccagcc tttggccctc aaggctccta tgaaaaagca
     3481 tctacagaag ttgctggacg aacttgctgc acaccccaac ttgctcattt gaaagactca
     3541 gtctgtcttg ccagtgatac taaacaattc gacagttcag gcagcccagc aaaaccacat
     3601 accaccctgc aagtgtcagg caggcaagga caacctacct gtgactgtga ctctgtcccg
     3661 ccaggaacac ctccacccat tgagtcattc acttttaaag aaaatcagct ctgtagaaac
     3721 acatgccagg agctgcataa acattccgtc gaacagactg aaacagcaga gcttcctgct
     3781 tctgatagca taaacccagg caacctacaa ttggtttcag agttaaagaa tccttcaggt
     3841 tcctgttccg tagatgtgag tgccatgttt tgggaaagag ccggttgtaa agagccatgt
     3901 atcataactg cttgcgaaga tgtagtttct ctttggaaag ctctggatgc ttggcagtgg
     3961 gaaaaacttt atacctggca cttcgcagag gttccagtat tacagatagt tccagtgcct
     4021 gatgtgtata atctcgtgtg tgtagctttg ggaaatttgg aaatcagaga gatcagggca
     4081 ttgttttgtt cctctgatga tgaaagtgaa aagcaagtac tactgaagtc tggaaatata
     4141 aaagctgtgc ttggcctgac aaagaggagg ctagttagta gcagtgggac cctttctgat
     4201 caacaagtag aagtcatgac gtttgcagaa gatggaggag gcaaagaaaa ccaatttttg
     4261 atgccccctg aggagactat actaactttt gctgaggtcc aagggatgca agaagctctg
     4321 cttggtacta ctattatgaa caacattgtt atttggaatt taaaaactgg tcaactcctg
     4381 aaaaagatgc acattgatga ttcttaccaa gcttcagtct gtcacaaagc ctattctgaa
     4441 atggggcttc tctttattgt cctgagtcat ccctgtgcca aagagagtga gtcgttgcga
     4501 agccctgtgt ttcagctcat tgtgattaac cctaagacga ctctcagcgt gggtgtgatg
     4561 ctgtactgtc ttcctccagg gcaggctggc aggttcctgg aaggtgacgt gaaagatcac
     4621 tgtgcagcag caatcttgac ttctggaaca attgccattt gggacttact tctcggtcag
     4681 tgtactgccc tcctcccacc tgtctctgac caacattggt cttttgtgaa atggtcgggt
     4741 acagactctc atttgctggc tggacaaaaa gatggaaata tatttgtata ccactattca
     4801 ggctcgagcg gtgggagctc cggtgtgagc ggctggcggc tgttcaagaa gattagctaa
     4861 gtttaaacgg ccgcgactct agactgcagg catgcaagct gatttggctg ctgccaccgc
     4921 tgagcaaggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     4981 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     5041 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     5101 caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt
     5161 aaaatcttaa ttaagacttc taatataact tcgtatagca tacattatac gaagttatgg
     5221 ttccactgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     5281 gaggaactaa acccaggagg cagatcatga ttgaacaaga tggattgcac gcaggttctc
     5341 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5401 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5461 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     5521 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5581 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     5641 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5701 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5761 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     5821 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     5881 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     5941 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6001 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6061 agcgcatcgc cttctatcgc cttcttgacg agttcttctg attcgaaatg accgaccaag
     6121 cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6661 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     6721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6841 atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6901 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttata
     6961 gtccggaatc gtcacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat
     7021 tgcggccatc gtgatggcta gagtttagcc ctcccacaca taaccagagg gcagcaattc
     7081 acgaatccca actgccgtcg gctgtccatc actgtccttc actatggctt tgatcccagg
     7141 atgcagatcg agaagcacct gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc
     7201 atttccgatc gcgacgatac aagtcaggtt gccagctgcc gcagcagcag cagtgcccag
     7261 caccacgagt tctgcacaag gtcccccagt aaaatgatat acattgacac cagtgaagat
     7321 gcggccgtcg ctagagagag ctgcgctggc gacgctgtag tcttcagaga tggggatgct
     7381 gttgattgta gccgttgctc tttcaatgag ggtggattct tcttgagaca aaggcttggc
     7441 catggtggcg ctagtgtcag aagaatcgag ctttttgcaa aagcctaggc ctccaaaaaa
     7501 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     7561 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     7621 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     7681 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     7741 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     7801 cacattccac agctggttct ttccgcctca gaaggtacct aaccaagttc ctctttcaga
     7861 ggttatttca ggccatggtg ctgcgc
//
