LOCUS       sequence-425863-        8852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human GEMIN5. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_GEMIN5:1-1508
FEATURES             Location/Qualifiers
     source          1..8852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5915..6036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5926..5945)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(6151..6184)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6194..6241
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6273..7067
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6327..6346)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6937..6956
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(7225..7813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7305..7324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7939..7987
                     /note="synthetic polyadenylation signal"
     CDS             complement(8011..8409)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      8406..8415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8440..8797)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8454..8589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8606..8797
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8754..8774
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggggca ggagccgcgg
     1261 acgctgccgc cctcccccaa ctggtactgc gcccgctgca gcgatgccgt gcccgggggc
     1321 ctctttggct tcgccgcgcg gacctccgtc ttccttgtcc gcgtgggccc gggcgcaggc
     1381 gagagtccag ggacaccccc gtttcgagtc ataggagagt tggtgggaca caccgaaagg
     1441 gtctctggct tcacattttc tcatcaccct ggtcagtaca acctctgtgc caccagctcc
     1501 gacgatggga ctgtgaaaat atgggatgta gagacaaaaa cagttgtgac agaacatgca
     1561 ctccatcagc atacgatatc aacattacat tggtctcctc gagtaaagga cttaatagta
     1621 tctggggatg aaaaaggagt agttttctgt tactggttta acagaaatga cagccagcac
     1681 ctctttatag aacccaggac aattttctgt cttacttgtt cacctcatca tgaagattta
     1741 gtagccattg gctacaagga tggcatagtg gtgataattg acatcagtaa gaaaggagaa
     1801 gttattcata ggcttcgagg ccatgatgat gaaatccact ccatagcctg gtgtcccctg
     1861 cctggtgaag attgtttatc tataaaccaa gaggaaactt cagaagaagc tgaaattacc
     1921 aacgggaatg ctgtagcaca agctccagta acaaaaggtt gctacttagc cactggaagc
     1981 aaagatcaaa ccattcgaat ctggagctgt tctagaggcc gaggggtgat gattttgaaa
     2041 ttgccctttc tgaagagaag aggagggggt atagacccaa ctgttaaaga gcgcctttgg
     2101 ttgacactcc attggcccag caatcaacca acacagctgg tatctagctg ttttggaggt
     2161 gaactgttgc aatgggatct cactcaatct tggagacgga aatacaccct cttcagtgcc
     2221 tcatcagaag ggcaaaatca ttcaagaatt gtgtttaatt tatgtccttt acaaacagag
     2281 gatgacaaac agctgttact ttctacatca atggatagag atgtaaaatg ttgggacata
     2341 gccaccttgg agtgcagctg gacccttcct tcccttggtg ggtttgcata cagcctggct
     2401 ttctcttctg tggacatagg ctctttggcc ataggtgttg gggatggcat gatccgtgta
     2461 tggaatacac tctccataaa gaacaactat gatgtgaaaa atttttggca aggcgtgaag
     2521 tccaaggtta cagcgctgtg ctggcaccca accaaggaag gttgcttagc ttttggaact
     2581 gatgatggaa aagtgggatt gtatgacacc tactccaaca agcctccaca gatttctagc
     2641 acatatcata agaagactgt atatacgtta gcctgggggc caccagtacc ccccatgtca
     2701 cttggaggag aaggagacag accttccctt gctttataca gctgtggagg agaagggatt
     2761 gtcttacagc ataatccctg gaagcttagt ggagaagcct ttgacatcaa caaactcatc
     2821 agggacacca attcaatcaa atacaaattg cctgtacaca cagagataag ttggaaagca
     2881 gatggcaaaa tcatggctct tggcaatgaa gatggatcaa tagaaatatt tcagattccc
     2941 aacctgaaac tgatctgtac tatccaacag catcacaagc ttgtgaatac cattagctgg
     3001 catcatgagc atggcagcca gccagaattg agctatctga tggcctctgg ctccaacaat
     3061 gcagtcattt acgtgcacaa cctgaagact gtcatagaga gcagccctga gtctccagtg
     3121 accattacag agccctaccg gaccctctca gggcatacgg ccaagattac cagtgtggcg
     3181 tggagcccac atcatgatgg aaggctggta tctgcttcct atgatggtac agcccaggtg
     3241 tgggatgctc tccgggaaga gcccctgtgc aatttccgag gacatcaagg tcgactgctt
     3301 tgtgtggcat ggtctccttt ggatccagac tgcatctatt caggggcaga tgacttttgt
     3361 gtgcacaagt ggctcacttc catgcaagat cattcccggc ctcctcaagg caaaaaaagt
     3421 attgaattgg agaaaaaacg gctctctcaa cctaaggcaa agcccaaaaa gaagaaaaag
     3481 cccaccttga gaactcctgt aaagctggaa tcgattgatg gaaatgaaga agaaagcatg
     3541 aaggagaact caggacctgt tgagaatggt gtgtcagacc aagaagggga ggagcaagca
     3601 cgggagccgg aattaccctg tggccttgct ccagcggttt ctagagaacc agttatctgc
     3661 actccagttt cctcaggctt tgaaaagtca aaagtcacca ttaataacaa agtcatttta
     3721 ctgaaaaagg agccaccaaa agagaagcca gaaaccttaa tcaagaagag aaaagctcgt
     3781 tccttgcttc ccctgagtac aagcctggac cacagatcca aagaggagct tcatcaggac
     3841 tgtttggtac tagcaactgc aaagcactcc agagagctga atgaagatgt gtctgctgat
     3901 gttgaggaaa gatttcatct ggggcttttc acagacaggg ctaccctgta tagaatgatt
     3961 gatattgaag gaaaaggtca cttagaaaat ggccaccctg agttatttca ccagcttatg
     4021 ctttggaaag gagatctcaa aggtgttctc cagactgcag cagaaagagg ggagctgaca
     4081 gacaaccttg tggctatggc accagcagct ggctaccatg tgtggctatg ggctgtggaa
     4141 gcttttgcca aacagctgtg ttttcaggat cagtatgtca aggctgcttc tcacctactt
     4201 tccatccaca aagtgtatga agcggtggag ctgctcaagt caaaccattt ttacagggaa
     4261 gctattgcga ttgccaaggc ccggctgcgc ccggaggacc cagtcctgaa ggacttgtac
     4321 ctcagctggg gaaccgtcct agaaagagat ggccactatg ctgtagctgc caaatgctat
     4381 ttaggggcca cttgtgctta tgatgcagcc aaagttttgg ccaaaaaggg ggatgcggca
     4441 tcacttagaa cggctgcaga gttggctgcc atcgtaggag aggatgagtt gtctgcttcc
     4501 ctggctctca gatgtgccca agagctgctt ctggccaaca actgggtggg agcccaggaa
     4561 gccctgcagc tgcatgaaag tctacagggt cagagattgg tgttttgcct tctggagcta
     4621 ctgtccaggc atctggagga aaagcagctt tcagagggca aaagctcctc ctcttaccac
     4681 acttggaaca cgggcaccga agggcctttc gtggagaggg tgactgcagt gtggaagagc
     4741 atcttcagcc ttgacacccc tgagcagtat caggaagcct ttcagaagct gcagaacatc
     4801 aagtacccat ctgctacaaa taacacacct gccaaacagc tcctgcttca catttgccat
     4861 gacttgaccc tggcagtgct gagccaacag atggcctcct gggacgaggc tgtgcaggcg
     4921 ctccttcggg cggtggtccg gagctatgac tcagggagct tcaccatcat gcaggaagtg
     4981 tactcagcct ttctccctga tggctgtgac cacctaagag acaagttggg ggaccatcaa
     5041 tcccctgcca caccagcttt caaaagtttg gaggcctttt ttctttatgg gcgtctgtat
     5101 gaattctggt ggtctctctc cagaccttgc ccaaattcca gtgtctgggt aagggctggt
     5161 cacagaacac tctctgttga gccaagccag cagttagaca ctgccagcac tgaagaaacg
     5221 gaccctgaaa cttctcagcc agagccaaac aggccttcag aactagactt gagactcaca
     5281 gaagaaggtg agcgaatgct gagtactttt aaggagctct tttcagaaaa gcatgccagt
     5341 ctccaaaact cacagagaac tgttgctgaa gtccaagaga ccttggcaga aatgatccga
     5401 caacaccaaa agagtcaact ctgtaaatcc acagcaaatg gtcctgataa gaatgaaccg
     5461 gaagtagaag cagagcagcc cctctgcagt tctcagagcc agtgtaaaga agaaaaaaat
     5521 gagccacttt ctctgcctga gttaaccaaa aggcttaccg aggcaaatca gagaatggca
     5581 aaatttcctg agagcattaa ggcctggccc ttcccagatg tgctggagtg ctgcctcgtc
     5641 ctgcttctca tcaggtccca ctttcctggc tgtctggccc aggaaatgca gcagcaggcc
     5701 caagagctcc ttcagaaata cggcaacacg aaaacttaca gaagacactg ccagaccttc
     5761 tgtatgggct cgagcggtgg gagctccggt gtgagcggct ggcggctgtt caagaagatt
     5821 agctaagttt aaacggccgc gactctagac tgcaggcatg caagctgatt tggctgctgc
     5881 caccgctgag caaggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     5941 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     6001 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     6061 atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa cctctacaaa
     6121 tgtggtaaaa tcttaattaa gacttctaat ataacttcgt atagcataca ttatacgaag
     6181 ttatggttcc actgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     6241 caaggtgagg aactaaaccc aggaggcaga tcatgattga acaagatgga ttgcacgcag
     6301 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6361 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     6421 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     6481 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     6541 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     6601 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6661 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6721 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6781 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6841 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6901 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6961 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     7021 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgattc gaaatgaccg
     7081 accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7801 aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7921 tttatagtcc ggaatcgtca cacaaaaaac caacacacag atgtaatgaa aataaagata
     7981 ttttattgcg gccatcgtga tggctagagt ttagccctcc cacacataac cagagggcag
     8041 caattcacga atcccaactg ccgtcggctg tccatcactg tccttcacta tggctttgat
     8101 cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg caggggctca agatgcccct
     8161 gttctcattt ccgatcgcga cgatacaagt caggttgcca gctgccgcag cagcagcagt
     8221 gcccagcacc acgagttctg cacaaggtcc cccagtaaaa tgatatacat tgacaccagt
     8281 gaagatgcgg ccgtcgctag agagagctgc gctggcgacg ctgtagtctt cagagatggg
     8341 gatgctgttg attgtagccg ttgctctttc aatgagggtg gattcttctt gagacaaagg
     8401 cttggccatg gtggcgctag tgtcagaaga atcgagcttt ttgcaaaagc ctaggcctcc
     8461 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     8521 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     8581 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct
     8641 ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt
     8701 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa
     8761 ctgacacaca ttccacagct ggttctttcc gcctcagaag gtacctaacc aagttcctct
     8821 ttcagaggtt atttcaggcc atggtgctgc gc
//
