LOCUS       sequence-425867-        6359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR48. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_WDR48:1-677
FEATURES             Location/Qualifiers
     source          1..6359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1241..1250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3422..3543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3433..3452)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3658..3691)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3701..3748
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3780..4574
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3834..3853)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4444..4463
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4732..5320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4812..4831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5446..5494
                     /note="synthetic polyadenylation signal"
     CDS             complement(5518..5916)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5913..5922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5947..6304)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5961..6096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6113..6304
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6261..6281
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggcggc ccatcaccgg
     1261 cagaacacag cagggcggag gaaagtgcag gtttcctatg ttattcgaga tgaagtggag
     1321 aagtacaacc gaaatggagt caatgctctg cagctggatc cagcactaaa tagacttttc
     1381 acagccggtc gagactctat cataagaata tggagtgtca atcagcacaa gcaagatcca
     1441 tatatagcat ctatggaaca ccatactgat tgggtaaacg acattgtact ctgttgtaat
     1501 gggaaaacat taatatctgc ttcttctgac acgacagtaa aagtatggaa tgcacacaag
     1561 ggattttgca tgtcaacatt aaggacacat aaggattacg taaaggcctt agcatatgcc
     1621 aaggataaag aactagtagc atcagctggg ttggacagac aaatattcct ttgggatgtg
     1681 aatactctaa cagcattgac tgcctcaaat aacactgtca caacttcttc tttaagtgga
     1741 aacaaagatt ccatttatag cctggccatg aatcaactgg gaacaatcat tgtatcaggg
     1801 tccactgaaa aggtgttacg ggtatgggat ccaagaacat gtgcaaaact aatgaagctt
     1861 aaagggcaca cggataatgt gaaggcattg ctattaaaca gagatggcac gcaatgcctg
     1921 tcaggcagtt ctgatgggac aattcgcctt tggtcccttg gccagcagag atgtatagca
     1981 acataccgag tccatgatga aggtgtttgg gcgctgcaag tcaatgatgc cttcacacat
     2041 gtgtattctg gtggaaggga caggaagatt tattgtacag acctaagaaa ccctgacatt
     2101 cgggtgctaa tttgtgaaga aaaagcacca gttctcaaga tggagcttga tagatcagct
     2161 gatcctcctc ctgcaatttg ggttgcaaca actaagtcta cagtaaataa atggactttg
     2221 aaaggaattc ataattttag agcctctgga gattatgaca atgactgtac aaatcctata
     2281 acacctcttt gtacacaacc tgaccaggtt attaaagggg gtgctagtat tattcagtgc
     2341 cacattctta atgataagag acatatatta accaaagata ccaataataa tgtggcatat
     2401 tgggatgtat tgaaggcatg taaagttgaa gatctgggca aagtggattt tgaagatgaa
     2461 attaagaaaa gatttaaaat ggtgtatgtg ccaaattggt tctcagtaga cttaaaaaca
     2521 gggatgttaa ctattacttt ggatgaaagt gattgttttg ctgcctgggt ttctgcaaaa
     2581 gatgctggtt tcagcagccc tgatgggtca gatccaaaat tgaatttagg aggactttta
     2641 ctccaagcac tcctggaata ttggcctaga acacatgtga atccaatgga tgaagaggaa
     2701 aatgaagtaa accatgtaaa tggggagcag gagaaccgag tgcagaaggg aaatggatat
     2761 tttcaagtgc ccccacatac acccgtgatc tttggtgaag ctggaggtcg cacactgttc
     2821 aggctgctct gccgagattc cgggggtgag actgagtcta tgcttcttaa tgaaacagtg
     2881 ccacaatggg taattgacat cactgtggat aaaaatatgc ccaaattcaa caaaattcct
     2941 ttctacctcc aacctcatgc atcttcagga gcaaaaacct taaaaaaaga tagactctct
     3001 gctagtgaca tgctccaagt ccgaaaagtt atggaacatg tttatgaaaa aattatcaac
     3061 ttggataatg agtctcaaac cactagctct tctaataatg aaaaaccagg agaacaggaa
     3121 aaagaagaag atattgctgt gttggcagag gagaaaattg aacttttgtg ccaagaccag
     3181 gttttggatc caaatatgga ccttcgaaca gtgaaacact tcatatggaa gagcggtgga
     3241 gacctcaccc tccattaccg tcagaagtcc acgggctcga gcggtgggag ctccggtgtg
     3301 agcggctggc ggctgttcaa gaagattagc taagtttaaa cggccgcgac tctagactgc
     3361 aggcatgcaa gctgatttgg ctgctgccac cgctgagcaa ggccgcttcg agcagacatg
     3421 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3481 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3541 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     3601 tttttaagca agtaaaacct ctacaaatgt ggtaaaatct taattaagac ttctaatata
     3661 acttcgtata gcatacatta tacgaagtta tggttccact gttgacaatt aatcatcggc
     3721 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaacccagg aggcagatca
     3781 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     3841 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     3901 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3961 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4021 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4081 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4141 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4201 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     4261 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     4321 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     4381 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     4441 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     4501 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     4561 acgagttctt ctgattcgaa atgaccgacc aagcgacgcc caaccggtat cagctcactc
     4621 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4681 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4741 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4801 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4861 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4921 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4981 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5041 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5101 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5161 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5221 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5281 ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa gaagatcctt tgatcttttc
     5341 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5401 atcaaaaagg atcttcacct agatcctttt atagtccgga atcgtcacac aaaaaaccaa
     5461 cacacagatg taatgaaaat aaagatattt tattgcggcc atcgtgatgg ctagagttta
     5521 gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg tcggctgtcc
     5581 atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca cctgtcggca
     5641 ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga tacaagtcag
     5701 gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac aaggtccccc
     5761 agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga gagctgcgct
     5821 ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg ctctttcaat
     5881 gagggtggat tcttcttgag acaaaggctt ggccatggtg gcgctagtgt cagaagaatc
     5941 gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     6001 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     6061 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     6121 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     6181 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     6241 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc
     6301 tcagaaggta cctaaccaag ttcctctttc agaggttatt tcaggccatg gtgctgcgc
//
