LOCUS       sequence-425876-        6035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR20. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_WDR20:1-569
FEATURES             Location/Qualifiers
     source          1..6035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3098..3219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3109..3128)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3334..3367)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3377..3424
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3456..4250
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3510..3529)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4120..4139
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4408..4996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4488..4507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5122..5170
                     /note="synthetic polyadenylation signal"
     CDS             complement(5194..5592)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5589..5598
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5623..5980)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5637..5772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5789..5980
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5937..5957
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatggcgac ggagggagga
     1261 gggaaggaga tgaacgagat taagacccaa ttcaccaccc gggaaggtct gtacaagctg
     1321 ctgccgcact cggagtacag ccggcccaac cgggtgccct tcaactcgca gggatccaac
     1381 cctgtccgcg tctccttcgt aaacctcaac gaccagtctg gcaacggcga ccgcctctgc
     1441 ttcaatgtgg gccgggagct gtacttctat atctacaagg gggtccgcaa ggctgctgac
     1501 ttgagtaaac caatagataa aaggatatac aaaggaacac agcctacttg tcatgacttc
     1561 aaccacctaa cagccacagc agaaagtgtc tctctcctag tgggcttttc cgcaggccaa
     1621 gtccagctta tagacccaat caaaaaagaa actagcaaac tttttaatga ggaaagacta
     1681 atagacaagt cacgagttac ctgtgtcaaa tgggttcacg gttcggaaag ccttttccta
     1741 gtagcccact cgagtgggaa catgtactta tataatgtgg agcacacttg tggcaccaca
     1801 gccccccact accagcttct gaagcaggga gagagctttg ccgtgcacac ttgcaagagc
     1861 aaatccacga ggaaccctct ccttaagtgg acggtgggcg agggggccct caacgagttt
     1921 gctttctccc cagatggcaa gttcttagcg tgcgtgagcc aggacgggtt tctgcgggtg
     1981 ttcaactttg actcagtgga gctgcacggt acgatgaaaa gctactttgg gggcttgctg
     2041 tgtgtgtgct ggagcccgga tggcaagtac atcgtgacag gtggggagga cgacttggtg
     2101 acagtctggt cctttgtaga ctgccgagta atagccagag gccacgggca caagtcctgg
     2161 gtcagtgttg tagcgtttga cccttatacc actagtgtag aagaaggtga ccctatggag
     2221 tttagtggca gcgatgagga cttccaagac cttcttcatt ttggcagaga tcgagcaaat
     2281 agtacacagt ccaggctctc caaacggaac tctacagaca gccgccccgt aagtgtcacg
     2341 tatcggtttg gttccgtggg ccaggacaca cagctctgtt tatgggacct tacagaagat
     2401 atccttttcc ctcaccaacc cctctcaaga gcaaggacac acacaaatgt catgaatgcc
     2461 acgagtcctc ctgctggaag caatgggaac agtgttacaa cacccgggaa ctctgtgccg
     2521 cctcctctgc cacggtccaa cagccttcca cattcagcag tctcaaatgc tggcagcaaa
     2581 agcagtgtca tggacggggc cattgcttct ggggtcagca aatttgcaac actttcacta
     2641 catgaccgga aggagaggca ccacgagaaa gatcacaagc gaaatcatag catgggacac
     2701 atttctagca agagcagtga caaactgaat ctagttacca aaaccaaaac ggaccctgct
     2761 aaaactctgg gaacgcccct gtgtcctcga atggaagatg ttcccttgtt agagccgctg
     2821 atatgtaaaa agatagcaca tgagagactg actgtactaa tatttcttga agactgtata
     2881 gtcactgctt gtcaggaggg atttatttgc acatggggaa ggcctggtaa agtggtaagt
     2941 tttaatcctg gctcgagcgg tgggagctcc ggtgtgagcg gctggcggct gttcaagaag
     3001 attagctaag tttaaacggc cgcgactcta gactgcaggc atgcaagctg atttggctgc
     3061 tgccaccgct gagcaaggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     3121 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     3181 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     3241 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta aaacctctac
     3301 aaatgtggta aaatcttaat taagacttct aatataactt cgtatagcat acattatacg
     3361 aagttatggt tccactgttg acaattaatc atcggcatag tatatcggca tagtataata
     3421 cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat ggattgcacg
     3481 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3541 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3601 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     3661 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3721 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3781 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3841 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3901 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3961 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     4021 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4081 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4141 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4201 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ttcgaaatga
     4261 ccgaccaagc gacgcccaac cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4741 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4801 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4861 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4921 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4981 aaaaaaagga tttcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5041 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5101 ccttttatag tccggaatcg tcacacaaaa aaccaacaca cagatgtaat gaaaataaag
     5161 atattttatt gcggccatcg tgatggctag agtttagccc tcccacacat aaccagaggg
     5221 cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca ctatggcttt
     5281 gatcccagga tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc tcaagatgcc
     5341 cctgttctca tttccgatcg cgacgataca agtcaggttg ccagctgccg cagcagcagc
     5401 agtgcccagc accacgagtt ctgcacaagg tcccccagta aaatgatata cattgacacc
     5461 agtgaagatg cggccgtcgc tagagagagc tgcgctggcg acgctgtagt cttcagagat
     5521 ggggatgctg ttgattgtag ccgttgctct ttcaatgagg gtggattctt cttgagacaa
     5581 aggcttggcc atggtggcgc tagtgtcaga agaatcgagc tttttgcaaa agcctaggcc
     5641 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     5701 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     5761 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     5821 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     5881 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     5941 taactgacac acattccaca gctggttctt tccgcctcag aaggtaccta accaagttcc
     6001 tctttcagag gttatttcag gccatggtgc tgcgc
//
