LOCUS       sequence-425886-        7748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DDB1. C-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_DDB1:1-1140
FEATURES             Location/Qualifiers
     source          1..7748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4811..4932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4822..4841)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(5047..5080)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5090..5137
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5169..5963
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5223..5242)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5833..5852
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(6121..6709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6201..6220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6835..6883
                     /note="synthetic polyadenylation signal"
     CDS             complement(6907..7305)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      7302..7311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7336..7693)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7350..7485
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7502..7693
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7650..7670
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgtcgta caactacgtg
     1261 gtaacggccc agaagcccac cgccgtgaac ggctgcgtga ccggacactt tacttcggcc
     1321 gaagacttaa acctgttgat tgccaaaaac acgagattag agatctatgt ggtcaccgcc
     1381 gaggggcttc ggcccgtcaa agaggtgggc atgtatggga agattgcggt catggagctt
     1441 ttcaggccca agggggagag caaggacctg ctgtttatct tgacagcgaa gtacaatgcc
     1501 tgcatcctgg agtataaaca gagtggcgag agcattgaca tcattacgcg agcccatggc
     1561 aatgtccagg accgcattgg ccgcccctca gagaccggca ttattggcat cattgaccct
     1621 gagtgccgga tgattggcct gcgtctctat gatggccttt tcaaggttat tccactagat
     1681 cgcgataata aagaactcaa ggccttcaac atccgcctgg aggagctgca tgtcattgat
     1741 gtcaagttcc tatatggttg ccaagcacct actatttgct ttgtctacca ggaccctcag
     1801 gggcggcacg taaaaaccta tgaggtgtct ctccgagaaa aggaattcaa taagggccct
     1861 tggaaacagg aaaatgtcga agctgaagct tccatggtga tcgcagtccc agagcccttt
     1921 gggggggcca tcatcattgg acaggagtca atcacctatc acaatggtga caaatacctg
     1981 gctattgccc ctcctatcat caagcaaagc acgattgtgt gccacaatcg agtggaccct
     2041 aatggctcaa gatacctgct gggagacatg gaaggccggc tcttcatgct gcttttggag
     2101 aaggaggaac agatggatgg caccgtcact ctcaaggatc tccgtgtaga actccttgga
     2161 gagacctcta ttgctgagtg cttgacatac cttgataatg gtgttgtgtt tgtcgggtct
     2221 cgcctgggtg actcccagct tgtgaagctc aacgttgaca gtaatgaaca aggctcctat
     2281 gtagtggcca tggaaacctt taccaactta ggacccattg tcgatatgtg cgtggtggac
     2341 ctggagaggc aggggcaggg gcagctggtc acttgctctg gggctttcaa ggaaggttct
     2401 ttgcggatca tccggaatgg aattggaatc cacgagcatg ccagcattga cttaccaggc
     2461 atcaaaggat tatggccact gcggtctgac cctaatcgtg agactgatga cactttggtg
     2521 ctctcttttg tgggccagac aagagttctc atgttaaatg gagaggaggt agaagaaacc
     2581 gaactgatgg gtttcgtgga tgatcagcag actttcttct gtggcaacgt ggctcatcag
     2641 cagcttatcc agatcacttc agcatcggtg aggttggtct ctcaagaacc caaagctctg
     2701 gtcagtgaat ggaaggagcc tcaggccaag aacatcagtg tggcctcctg caatagcagc
     2761 caggtggtgg tggctgtagg cagggccctc tactatctgc agatccatcc tcaggagctc
     2821 cggcagatca gccacacaga gatggaacat gaagtggctt gcttggacat caccccatta
     2881 ggagacagca atggactgtc ccctctttgt gccattggcc tctggacgga catctcggct
     2941 cgtatcttga agttgccctc ttttgaacta ctgcacaagg agatgctggg tggagagatc
     3001 attcctcgct ccatcctgat gaccaccttt gagagtagcc attacctcct ttgtgccttg
     3061 ggagatggag cgcttttcta ctttgggctc aacattgaga caggtctgtt gagcgaccgt
     3121 aagaaggtga ctttgggcac ccagcccacc gtattgagga cttttcgttc tctttctacc
     3181 accaacgtct ttgcttgttc tgaccgcccc actgtcatct atagcagcaa ccacaaattg
     3241 gtcttctcaa atgtcaacct caaggaagtg aactacatgt gtcccctcaa ttcagatggc
     3301 tatcctgaca gcctggcgct ggccaacaat agcaccctca ccattggcac catcgatgag
     3361 atccagaagc tgcacattcg cacagttccc ctctatgagt ctccaaggaa gatctgctac
     3421 caggaagtgt cccagtgttt cggggtcctc tccagccgca ttgaagtcca agacacgagt
     3481 gggggcacga cagccttgag gcccagcgct agcacccagg ctctgtccag cagtgtaagc
     3541 tccagcaagc tgttctccag cagcactgct cctcatgaga cctcctttgg agaagaggtg
     3601 gaggtgcaca acctacttat cattgaccaa cacacctttg aagtgcttca tgcccaccag
     3661 tttctgcaga atgaatatgc cctcagtctg gtttcctgca agctgggcaa agaccccaac
     3721 acttacttca ttgtgggcac agcaatggtg tatcctgaag aggcagagcc caagcagggt
     3781 cgcattgtgg tctttcagta ttcggatgga aaactacaga ctgtggctga aaaggaagtg
     3841 aaaggggccg tgtactctat ggtggaattt aacgggaagc tgttagccag catcaatagc
     3901 acggtgcggc tctatgagtg gacaacagag aaggagctgc gcactgagtg caaccactac
     3961 aacaacatca tggccctcta cctgaagacc aagggcgact tcatcctggt gggcgacctt
     4021 atgcgctcag tgctgctgct tgcctacaag cccatggaag gaaactttga agagattgct
     4081 cgagacttta atcccaactg gatgagtgct gtggaaatct tggatgatga caattttctg
     4141 ggggctgaaa atgcctttaa cttgtttgtg tgtcaaaagg atagcgctgc caccactgac
     4201 gaggagcggc agcacctcca ggaggttggt cttttccacc tgggcgagtt tgtcaatgtc
     4261 ttttgccacg gctctctggt aatgcagaat ctgggtgaga cttccacccc cacacaaggc
     4321 tcggtgctct tcggcacggt caacggcatg atagggctgg tgacctcact gtcagagagc
     4381 tggtacaacc tcctgctgga catgcagaat cgactcaata aagtcatcaa aagtgtgggg
     4441 aagatcgagc actccttctg gagatccttt cacaccgagc ggaagacaga accagccaca
     4501 ggtttcatcg acggtgactt gattgagagt ttcctggata ttagccgccc caagatgcag
     4561 gaggtggtgg caaacctaca gtatgacgat ggcagcggta tgaagcgaga ggccactgca
     4621 gacgacctca tcaaggttgt ggaggagcta actcggatcc atggctcgag cggtgggagc
     4681 tccggtgtga gcggctggcg gctgttcaag aagattagct aagtttaaac ggccgcgact
     4741 ctagactgca ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga
     4801 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     4861 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     4921 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     4981 tgggaggttt ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagact
     5041 tctaatataa cttcgtatag catacattat acgaagttat ggttccactg ttgacaatta
     5101 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga
     5161 ggcagatcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5221 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5281 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5341 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5401 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5461 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5521 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5581 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5641 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5701 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5761 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5821 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5881 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5941 gccttcttga cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc
     6001 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6061 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6121 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6181 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6241 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6301 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6361 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6421 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6481 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6541 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6601 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6661 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
     6721 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6781 catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca
     6841 aaaaaccaac acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc
     6901 tagagtttag ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt
     6961 cggctgtcca tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac
     7021 ctgtcggcac cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat
     7081 acaagtcagg ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca
     7141 aggtccccca gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag
     7201 agctgcgctg gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc
     7261 tctttcaatg agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc
     7321 agaagaatcg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     7381 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     7441 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     7501 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     7561 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     7621 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     7681 ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg
     7741 tgctgcgc
//
