LOCUS       sequence-425887-        6866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human DDB1. loop deletion amino 
            acids I396-D689. C-terminal HiBiT tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-C_DDB1:1-1140
FEATURES             Location/Qualifiers
     source          1..6866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3929..4050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3940..3959)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4165..4198)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4208..4255
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4287..5081
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4341..4360)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4951..4970
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(5239..5827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5319..5338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5953..6001
                     /note="synthetic polyadenylation signal"
     CDS             complement(6025..6423)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6420..6429
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6454..6811)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6468..6603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6620..6811
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6768..6788
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggcga tcgcaacaag cttcgggaag
     1201 atctggagct agcggcgggg gatccggtgg gaattcgaca ccatgtcgta caactacgtg
     1261 gtaacggccc agaagcccac cgccgtgaac ggctgcgtga ccggacactt tacttcggcc
     1321 gaagacttaa acctgttgat tgccaaaaac acgagattag agatctatgt ggtcaccgcc
     1381 gaggggcttc ggcccgtcaa agaggtgggc atgtatggga agattgcggt catggagctt
     1441 ttcaggccca agggggagag caaggacctg ctgtttatct tgacagcgaa gtacaatgcc
     1501 tgcatcctgg agtataaaca gagtggcgag agcattgaca tcattacgcg agcccatggc
     1561 aatgtccagg accgcattgg ccgcccctca gagaccggca ttattggcat cattgaccct
     1621 gagtgccgga tgattggcct gcgtctctat gatggccttt tcaaggttat tccactagat
     1681 cgcgataata aagaactcaa ggccttcaac atccgcctgg aggagctgca tgtcattgat
     1741 gtcaagttcc tatatggttg ccaagcacct actatttgct ttgtctacca ggaccctcag
     1801 gggcggcacg taaaaaccta tgaggtgtct ctccgagaaa aggaattcaa taagggccct
     1861 tggaaacagg aaaatgtcga agctgaagct tccatggtga tcgcagtccc agagcccttt
     1921 gggggggcca tcatcattgg acaggagtca atcacctatc acaatggtga caaatacctg
     1981 gctattgccc ctcctatcat caagcaaagc acgattgtgt gccacaatcg agtggaccct
     2041 aatggctcaa gatacctgct gggagacatg gaaggccggc tcttcatgct gcttttggag
     2101 aaggaggaac agatggatgg caccgtcact ctcaaggatc tccgtgtaga actccttgga
     2161 gagacctcta ttgctgagtg cttgacatac cttgataatg gtgttgtgtt tgtcgggtct
     2221 cgcctgggtg actcccagct tgtgaagctc aacgttgaca gtaatgaaca aggctcctat
     2281 gtagtggcca tggaaacctt taccaactta ggacccattg tcgatatgtg cgtggtggac
     2341 ctggagaggc aggggcaggg gcagctggtc acttgctctg gggctttcaa ggaaggttct
     2401 ttgcggatca tccggaatgg aattggaagc ctggcgctgg ccaacaatag caccctcacc
     2461 attggcacca tcgatgagat ccagaagctg cacattcgca cagttcccct ctatgagtct
     2521 ccaaggaaga tctgctacca ggaagtgtcc cagtgtttcg gggtcctctc cagccgcatt
     2581 gaagtccaag acacgagtgg gggcacgaca gccttgaggc ccagcgctag cacccaggct
     2641 ctgtccagca gtgtaagctc cagcaagctg ttctccagca gcactgctcc tcatgagacc
     2701 tcctttggag aagaggtgga ggtgcacaac ctacttatca ttgaccaaca cacctttgaa
     2761 gtgcttcatg cccaccagtt tctgcagaat gaatatgccc tcagtctggt ttcctgcaag
     2821 ctgggcaaag accccaacac ttacttcatt gtgggcacag caatggtgta tcctgaagag
     2881 gcagagccca agcagggtcg cattgtggtc tttcagtatt cggatggaaa actacagact
     2941 gtggctgaaa aggaagtgaa aggggccgtg tactctatgg tggaatttaa cgggaagctg
     3001 ttagccagca tcaatagcac ggtgcggctc tatgagtgga caacagagaa ggagctgcgc
     3061 actgagtgca accactacaa caacatcatg gccctctacc tgaagaccaa gggcgacttc
     3121 atcctggtgg gcgaccttat gcgctcagtg ctgctgcttg cctacaagcc catggaagga
     3181 aactttgaag agattgctcg agactttaat cccaactgga tgagtgctgt ggaaatcttg
     3241 gatgatgaca attttctggg ggctgaaaat gcctttaact tgtttgtgtg tcaaaaggat
     3301 agcgctgcca ccactgacga ggagcggcag cacctccagg aggttggtct tttccacctg
     3361 ggcgagtttg tcaatgtctt ttgccacggc tctctggtaa tgcagaatct gggtgagact
     3421 tccaccccca cacaaggctc ggtgctcttc ggcacggtca acggcatgat agggctggtg
     3481 acctcactgt cagagagctg gtacaacctc ctgctggaca tgcagaatcg actcaataaa
     3541 gtcatcaaaa gtgtggggaa gatcgagcac tccttctgga gatcctttca caccgagcgg
     3601 aagacagaac cagccacagg tttcatcgac ggtgacttga ttgagagttt cctggatatt
     3661 agccgcccca agatgcagga ggtggtggca aacctacagt atgacgatgg cagcggtatg
     3721 aagcgagagg ccactgcaga cgacctcatc aaggttgtgg aggagctaac tcggatccat
     3781 ggctcgagcg gtgggagctc cggtgtgagc ggctggcggc tgttcaagaa gattagctaa
     3841 gtttaaacgg ccgcgactct agactgcagg catgcaagct gatttggctg ctgccaccgc
     3901 tgagcaaggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     3961 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4021 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4081 caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt
     4141 aaaatcttaa ttaagacttc taatataact tcgtatagca tacattatac gaagttatgg
     4201 ttccactgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     4261 gaggaactaa acccaggagg cagatcatga ttgaacaaga tggattgcac gcaggttctc
     4321 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4381 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4441 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     4501 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     4561 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     4621 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     4681 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     4741 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     4801 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     4861 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     4921 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     4981 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     5041 agcgcatcgc cttctatcgc cttcttgacg agttcttctg attcgaaatg accgaccaag
     5101 cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5401 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5641 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     5701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5821 atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttata
     5941 gtccggaatc gtcacacaaa aaaccaacac acagatgtaa tgaaaataaa gatattttat
     6001 tgcggccatc gtgatggcta gagtttagcc ctcccacaca taaccagagg gcagcaattc
     6061 acgaatccca actgccgtcg gctgtccatc actgtccttc actatggctt tgatcccagg
     6121 atgcagatcg agaagcacct gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc
     6181 atttccgatc gcgacgatac aagtcaggtt gccagctgcc gcagcagcag cagtgcccag
     6241 caccacgagt tctgcacaag gtcccccagt aaaatgatat acattgacac cagtgaagat
     6301 gcggccgtcg ctagagagag ctgcgctggc gacgctgtag tcttcagaga tggggatgct
     6361 gttgattgta gccgttgctc tttcaatgag ggtggattct tcttgagaca aaggcttggc
     6421 catggtggcg ctagtgtcag aagaatcgag ctttttgcaa aagcctaggc ctccaaaaaa
     6481 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     6541 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     6601 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     6661 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     6721 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     6781 cacattccac agctggttct ttccgcctca gaaggtacct aaccaagttc ctctttcaga
     6841 ggttatttca ggccatggtg ctgcgc
//
