LOCUS       sequence-425889-        6762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human ELP2. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_ELP2:1-826
FEATURES             Location/Qualifiers
     source          1..6762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3825..3946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3836..3855)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4061..4094)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4104..4151
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4183..4977
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4237..4256)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4847..4866
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(5135..5723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5215..5234)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5849..5897
                     /note="synthetic polyadenylation signal"
     CDS             complement(5921..6319)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6316..6325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6350..6707)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6364..6499
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6516..6707
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6664..6684
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggtggcacc
     1261 cgtgctggag acttctcacg tgttttgctg cccaaaccgg gtgcggggag tcctgaactg
     1321 gagctctggg cccagaggac ttctggcctt tggcacgtcc tgctccgtgg tgctctatga
     1381 ccccctgaaa agggttgttg ttaccaactt gaatggtcac accgcccgag tcaattgcat
     1441 acagtggatt tgtaaacagg atggctcccc ttctactgaa ttagtttctg gaggatctga
     1501 taatcaagtg attcactggg aaatagagga taatcagctt ttaaaagcag tgcatcttca
     1561 aggccatgaa ggacctgttt atgcggtgca tgctgtttac cagaggagga catcagatcc
     1621 tgcattatgt ccactgatcg tttctgcagc tgcagattct gctgttcgac tctggtctaa
     1681 aaagggtcca gaagtaatgt gccttcagac tttaaacttt ggaaatggat ttgctttggc
     1741 tctctgctta tcttttttgc caaatactga tgtaccaata ttagcatgtg gcaatgatga
     1801 ttgcagaatt cacatatttg ctcaacaaaa tgatcagttt cagaaagtgc tttctctctg
     1861 tggacatgag gattggatta gaggagtgga atgggcagcc tttggtagag atcttttcct
     1921 agcaagctgt tcacaagatt gcctgataag aatatggaag ctgtatataa agtcaacatc
     1981 tttagaaact caggatgacg ataacataag actgaaagaa aatactttta ccatagaaaa
     2041 tgaaagtgtt aaaatagcat ttgctgttac tctggagaca gtgctagccg gtcatgaaaa
     2101 ctgggtaaat gcagttcact ggcaacctgt gttttacaaa gatggtgtcc tacagcagcc
     2161 aatgagatta ttatctgctt ccatggataa aaccatgatt ctctgggctc cagatgaaga
     2221 gtcaggagtt tggctagaac aggttcgagt aggtgaagta ggtgggaata ctttgggatt
     2281 ttatgattgc cagttcaatg aagatggctc catgatcatt gctcatgctt tccacggagc
     2341 gttgcacctt tggaaacaga atacagttaa cccaagagag tggactccag agattgtcat
     2401 ttcaggacac tttgatggtg tccaagacct agtctgggat ccagaaggag aatttattat
     2461 cactgttggt actgatcaga caactagact ttttgctcca tggaagagaa aagaccaatc
     2521 acaggtgact tggcatgaaa ttgcaaggcc tcagatacat gggtatgacc tgaaatgttt
     2581 ggcaatgatt aatcggtttc agtttgtatc tggagcagat gaaaaagttc ttcgggtttt
     2641 ttctgcacct cggaattttg tggaaaattt ttgtgccatt acaggacaat cactgaatca
     2701 tgtgctctgt aatcaagata gtgatcttcc agaaggagcc actgtccctg cattgggatt
     2761 atcaaataaa gctgtctttc agggagatat agcttctcag ccttctgatg aagaggagct
     2821 gttaactagt actggttttg agtatcagca ggtggccttt cagccctcca tacttactga
     2881 gcctcccact gaggatcatc ttctgcagaa tactttgtgg cctgaagttc aaaaactata
     2941 tgggcacggt tatgaaatat tttgtgttac ttgtaacagt tcaaagactc tgcttgcctc
     3001 agcttgtaag gcagctaaga aagagcatgc agctatcatt ctttggaaca ctacatcttg
     3061 gaaacaggtg cagaatttag ttttccacag tttgacagtc acgcagatgg ccttctcacc
     3121 taatgagaag ttcttactag ctgtttccag agatcgaacc tggtcattgt ggaaaaagca
     3181 ggatacaatc tcacctgagt tcgagccagt ttttagtctt tttgccttca ccaacaaaat
     3241 tacttctgtg cacagtagaa ttatttggtc ttgtgattgg agtcctgaca gcaagtattt
     3301 cttcactggg agtcgagaca aaaaggtggt tgtctggggt gagtgtgact ccactgatga
     3361 ctgtattgag cacaacattg gcccctgctc ctcagtcctg gacgtgggtg gggctgtgac
     3421 agctgtcagc gtctgcccag tgctccaccc ttctcaacga tacgtggttg cagtaggatt
     3481 ggagtgtgga aagatttgct tatatacctg gaaaaagact gatcaagttc cagaaataaa
     3541 tgactggacc cactgtgtag aaacaagtca aagccaaagt catacactgg ctatcagaaa
     3601 attatgctgg aagaattgca gtggaaaaac tgaacagaag gaagcagaag gtgctgagtg
     3661 gttacacttt gcaagctgtg gtgaagatca cactgtgaag atacacagag tcaataaatg
     3721 tgcactgtaa tctagaggtc aagcttcggg ggtttaaacc tgcaggcatg caagctgatt
     3781 tggctgctgc caccgctgag caaggccgct tcgagcagac atgataagat acattgatga
     3841 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3901 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     3961 cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa gcaagtaaaa
     4021 cctctacaaa tgtggtaaaa tcttaattaa gacttctaat ataacttcgt atagcataca
     4081 ttatacgaag ttatggttcc actgttgaca attaatcatc ggcatagtat atcggcatag
     4141 tataatacga caaggtgagg aactaaaccc aggaggcaga tcatgattga acaagatgga
     4201 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     4261 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     4321 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     4381 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     4441 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     4501 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     4561 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     4621 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     4681 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     4741 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     4801 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     4861 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     4921 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgattc
     4981 gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt
     5041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5401 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5461 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5521 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     5581 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5641 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5701 cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5761 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5821 cctagatcct tttatagtcc ggaatcgtca cacaaaaaac caacacacag atgtaatgaa
     5881 aataaagata ttttattgcg gccatcgtga tggctagagt ttagccctcc cacacataac
     5941 cagagggcag caattcacga atcccaactg ccgtcggctg tccatcactg tccttcacta
     6001 tggctttgat cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg caggggctca
     6061 agatgcccct gttctcattt ccgatcgcga cgatacaagt caggttgcca gctgccgcag
     6121 cagcagcagt gcccagcacc acgagttctg cacaaggtcc cccagtaaaa tgatatacat
     6181 tgacaccagt gaagatgcgg ccgtcgctag agagagctgc gctggcgacg ctgtagtctt
     6241 cagagatggg gatgctgttg attgtagccg ttgctctttc aatgagggtg gattcttctt
     6301 gagacaaagg cttggccatg gtggcgctag tgtcagaaga atcgagcttt ttgcaaaagc
     6361 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     6421 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     6481 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     6541 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
     6601 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     6661 cacaccctaa ctgacacaca ttccacagct ggttctttcc gcctcagaag gtacctaacc
     6721 aagttcctct ttcagaggtt atttcaggcc atggtgctgc gc
//
