LOCUS       sequence-425906-        7017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human GTF3C2. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_GTF3C2:1-911
FEATURES             Location/Qualifiers
     source          1..7017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4080..4201
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4091..4110)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4316..4349)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4359..4406
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4438..5232
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4492..4511)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5102..5121
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(5390..5978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5470..5489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6104..6152
                     /note="synthetic polyadenylation signal"
     CDS             complement(6176..6574)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6571..6580
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6605..6962)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6619..6754
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6771..6962
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6919..6939
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggatacctg
     1261 cggggtcggc tatgttgccc tgggggaggc cggccccgtg gggaacatga ctgtggtaga
     1321 ctctcctgga caagaggtgc taaatcagct tgatgtcaag acctcttcag aaatgaccag
     1381 tgcagaggct tccgtagaga tgtcattacc tacccctttg cctggatttg aggattctcc
     1441 tgatcagagg aggctccctc cagagcagga aagcctctcc agactggaac agccagatct
     1501 ttcttcagag atgtcaaagg tctcaaagcc tagggcctca aagcctggcc ggaagagagg
     1561 tggtaggaca cgaaaaggcc ccaaaaggcc ccaacagcct aatcctccat cagccccact
     1621 ggttcctggt ctcttagatc aatccaaccc tctgtccacc cccatgccta agaaacgagg
     1681 tcgaaagtcc aaggcagagc tgctgctgct gaagttgtca aaagacctag atcggccaga
     1741 atctcaatct ccaaagaggc cccctgagga ctttgagacc ccttctgggg aacgaccccg
     1801 ccgaagggct gcccaagtgg cacttctgta tcttcaggaa ctggctgaag agctctcaac
     1861 agccctgcct gcccctgtgt cctgtcctga gggccccaag gtgagcagcc ccaccaaacc
     1921 gaagaagatc cggcagccag cagcctgtcc aggtggagaa gaggtggatg gtgctccacg
     1981 ggatgaagac ttttttctcc aggttgaggc tgaagatgtg gaagaaagtg agggcccaag
     2041 tgagagctca tctgaacctg agcctgtagt gccccgaagc accccacgag gatctacttc
     2101 agggaaacag aaaccacact gccgaggaat ggctcccaat ggcttaccag atcatatcat
     2161 ggctcctgtt tggaagtgcc tccatctcac caaggacttc cgagagcaga aacattcata
     2221 ctgggagttt gctgagtgga ttcctttagc ctggaagtgg cacttgttat ctgagcttga
     2281 ggccgctccc tacctgcccc aggaggagaa gtctccattg ttttctgtac aacgtgaagg
     2341 gctacctgaa gatggcaccc tctaccgaat aaacagattt agctcgatca cagcacatcc
     2401 agagcgctgg gatgtgtcct tcttcacggg gggaccgctc tgggctctgg actggtgccc
     2461 agtgccagag ggggcaggag cctcgcaata tgtggctctt ttctccagcc ctgacatgaa
     2521 tgagacacac ccactgagcc agcttcattc gggtcctggg ctgctccagc tctggggcct
     2581 tgggaccttg cagcaagaaa gctgtcctgg caacagggcc cactttgtct atgggattgc
     2641 ttgtgacaac ggctgcatct gggacctcaa gttctgcccc agtggagcat gggaacttcc
     2701 aggcacccct cggaaggctc ctctcctgcc ccggttgggt ctcttggctc tggcctgctc
     2761 agacgggaaa gtactgctat tcagtctacc ccatccggag gccctgctgg ctcagcaacc
     2821 cccagatgca gtgaagcctg ccatatataa ggtacaatgt gtggcaactc tgcaggtggg
     2881 gtctatgcaa gctacagacc cctctgagtg tggtcagtgc cttagcctgg cctggatgcc
     2941 taccaggccc caccaacacc tagctgctgg atattataat ggcatggtgg ttttctggaa
     3001 ccttcccact aactcacccc tgcagcggat acggctctct gatggctcct taaagctcta
     3061 ccccttccag tgtttcctag cccatgacca ggctgtgcgt acccttcaat ggtgcaaagc
     3121 taacagccat ttccttgtct ctgcggggag tgaccggaaa atcaaattct gggaccttcg
     3181 acgtccttac gaacccataa actctatcaa gcgcttcttg agtacagaac tggcctggct
     3241 gcttccctac aatggtgtca ctgtggctca ggacaactgc tatgcctctt atggactctg
     3301 tgggattcat tatattgacg ctggttacct tggtttcaag gcctacttca ctgctcctcg
     3361 aaaaggcacc gtttggagtc tttcaggatc cgactggctt gggacaatag ctgcaggaga
     3421 tatatccggg gagctcattg ctgctatatt accagatatg gcactgaatc caataaatgt
     3481 caagcgacct gtagagcgaa gatttcctat atataaagca gatctgatac cgtatcagga
     3541 cagtcctgaa ggtccagacc attcttctgc ttcatctggg gtccccaacc ctcctaaggc
     3601 tcgaacttac actgaaactg tcaaccatca ctacttgctc tttcaagaca cagatttggg
     3661 ttcattccat gatctgctcc gtagagaacc aatgctgcgc atgcaggagg gagaggggca
     3721 ttctcaactc tgcctggaca ggctgcagct ggaggctatt cataaggtac gtttcagccc
     3781 aaacctggac tcctatggat ggctggtatc tggggggcag tcagggctgg ttcgaatcca
     3841 ttttgtccgt ggactcgcct ccccactggg ccaccgtatg cagcttgaaa gccgagccca
     3901 cttcaatgct atgttccaac catcctcccc cactagacgg cctggcttct ctccaaccag
     3961 ccatcgcctt ctgcccactc cctaatctag aggtcaagct tcgggggttt aaacctgcag
     4021 gcatgcaagc tgatttggct gctgccaccg ctgagcaagg ccgcttcgag cagacatgat
     4081 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4141 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4201 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     4261 tttaagcaag taaaacctct acaaatgtgg taaaatctta attaagactt ctaatataac
     4321 ttcgtatagc atacattata cgaagttatg gttccactgt tgacaattaa tcatcggcat
     4381 agtatatcgg catagtataa tacgacaagg tgaggaacta aacccaggag gcagatcatg
     4441 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4501 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4561 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     4621 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4681 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4741 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4801 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4861 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4921 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     4981 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5041 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5101 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5161 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5221 gagttcttct gattcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa
     5281 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5341 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5401 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5461 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5521 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5581 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5641 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5701 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5761 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5821 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5881 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5941 gcaagcagca gattacgcgc agaaaaaaag gatttcaaga agatcctttg atcttttcta
     6001 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6061 caaaaaggat cttcacctag atccttttat agtccggaat cgtcacacaa aaaaccaaca
     6121 cacagatgta atgaaaataa agatatttta ttgcggccat cgtgatggct agagtttagc
     6181 cctcccacac ataaccagag ggcagcaatt cacgaatccc aactgccgtc ggctgtccat
     6241 cactgtcctt cactatggct ttgatcccag gatgcagatc gagaagcacc tgtcggcacc
     6301 gtccgcaggg gctcaagatg cccctgttct catttccgat cgcgacgata caagtcaggt
     6361 tgccagctgc cgcagcagca gcagtgccca gcaccacgag ttctgcacaa ggtcccccag
     6421 taaaatgata tacattgaca ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg
     6481 cgacgctgta gtcttcagag atggggatgc tgttgattgt agccgttgct ctttcaatga
     6541 gggtggattc ttcttgagac aaaggcttgg ccatggtggc gctagtgtca gaagaatcga
     6601 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     6661 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     6721 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     6781 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     6841 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     6901 agcctgggga ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc
     6961 agaaggtacc taaccaagtt cctctttcag aggttatttc aggccatggt gctgcgc
//
