LOCUS       sequence-425907-        6249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human KATNB1. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_KATNB1:1-655
FEATURES             Location/Qualifiers
     source          1..6249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3162..3171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3312..3433
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3323..3342)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3548..3581)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3591..3638
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3670..4464
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3724..3743)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4334..4353
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4622..5210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4702..4721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5336..5384
                     /note="synthetic polyadenylation signal"
     CDS             complement(5408..5806)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5803..5812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5837..6194)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5851..5986
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6003..6194
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6151..6171
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggccacccc
     1261 tgtggtcacc aagacagcct ggaagttgca agagatcgtc gcgcatgcca gcaacgtgtc
     1321 ctcactggtg ctgggcaaag cctccgggcg gctgctggct acaggcgggg atgactgccg
     1381 cgtcaacctg tggtccatca acaagcccaa ctgcatcatg agcctgacgg gccacacatc
     1441 cccagtggag agcgtccgcc tcaacacccc cgaggagctc atcgtggccg gctctcagtc
     1501 gggctccatc cgtgtctggg acctggaagc tgccaaaatt cttcgcacac tcatgggaca
     1561 caaagccaac atctgcagcc tggatttcca cccgtacggc gagtttgtag cctctggttc
     1621 ccaggacaca aacatcaagc tctgggacat caggaggaaa ggctgtgtct tccgatacag
     1681 ggggcacagc caggccgtgc ggtgtctccg gttcagcccc gatgggaagt ggttggcgtc
     1741 ggccgcagat gaccacaccg tgaagctctg ggatctcact gccggcaaga tgatgtctga
     1801 gttccctggt cacacggggc ctgtcaacgt ggtcgagttt caccccaacg agtacctcct
     1861 ggcctccggc agctctgaca ggacaatccg cttctgggac ctggagaagt tccaggtggt
     1921 gagctgcatc gaaggggagc ctgggcccgt caggagcgtc ctcttcaacc cagatggctg
     1981 ctgcctgtac agcggctgcc aggactcact gcgtgtctac ggctgggaac ctgagcggtg
     2041 ctttgatgtg gtcctcgtca actggggcaa ggtggccgac ctggccatct gcaatgacca
     2101 gttgataggt gtggccttct cccagagcaa cgtctcctcc tacgtggtgg atctgacgcg
     2161 tgtcaccagg actggcacgg tggcccggga ccctgtgcag gaccaccggc ccctggcaca
     2221 gccactgccc aaccccagcg cccccctccg gcgcatctat gagcggccca gcacaacctg
     2281 cagcaagcct cagagggtga agcagaactc agagagcgag cgccgcagcc ccagcagcga
     2341 ggatgaccgg gacgagcgcg agtcccgcgc ggagatccag aacgccgagg actacaacga
     2401 gatcttccag cccaagaaca gcatcagtcg gacgccaccc cggagaagtg agcccttccc
     2461 tgcaccccca gaggacgacg cagccacagc aaaggaggca gcaaagccca gccctgccat
     2521 ggatgtgcag ttcccggtgc caaatctgga ggtcctgccc cggcccccag tggttgcttc
     2581 cacacctgca cccaaggctg agcctgccat catccctgcc acccggaacg agcccatcgg
     2641 gctgaaggcc tccgacttcc tgcccgccgt gaagatcccc cagcaggccg agctggtgga
     2701 cgaggatgcc atgtcacaga tccgcaaagg ccacgacacc atgtgtgtgg tgctcaccag
     2761 ccgccacaag aacctggaca ctgtgcgggc tgtgtggacc atgggcgaca tcaagacgtc
     2821 ggtggactcc gctgtggcca tcaacgacct gtcggtggtg gtggacctcc tgaacatcgt
     2881 caaccagaaa gcctccctgt ggaagctgga cctgtgcacc accgtcctgc cacagattga
     2941 gaagcttctg cagagcaagt atgagagcta cgtccagacg ggctgcacct ccctgaagct
     3001 gatcctgcag cggtttctgc ccctcatcac agacatgctg gcggccccac cctctgtggg
     3061 tgtggatatc agcagggagg agaggctgca taagtgccgg ctctgctaca agcagcttaa
     3121 gagcatcagc ggcctggtca agagcaagtc aggcctgagc ggccgccatg gcagtacctt
     3181 ccgcgagctg cacctgctca tggccagtct ggactaatct agaggtcaag cttcgggggt
     3241 ttaaacctgc aggcatgcaa gctgatttgg ctgctgccac cgctgagcaa ggccgcttcg
     3301 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     3361 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     3421 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     3481 gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatct taattaagac
     3541 ttctaatata acttcgtata gcatacatta tacgaagtta tggttccact gttgacaatt
     3601 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaacccagg
     3661 aggcagatca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     3721 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     3781 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     3841 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     3901 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     3961 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     4021 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     4081 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     4141 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     4201 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     4261 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     4321 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     4381 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     4441 cgccttcttg acgagttctt ctgattcgaa atgaccgacc aagcgacgcc caaccggtat
     4501 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4561 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4621 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4681 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4741 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4801 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4861 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4921 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4981 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5041 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     5101 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5161 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa gaagatcctt
     5221 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5281 tcatgagatt atcaaaaagg atcttcacct agatcctttt atagtccgga atcgtcacac
     5341 aaaaaaccaa cacacagatg taatgaaaat aaagatattt tattgcggcc atcgtgatgg
     5401 ctagagttta gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg
     5461 tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca
     5521 cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga
     5581 tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac
     5641 aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga
     5701 gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg
     5761 ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccatggtg gcgctagtgt
     5821 cagaagaatc gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct
     5881 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     5941 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     6001 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     6061 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     6121 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctggt
     6181 tctttccgcc tcagaaggta cctaaccaag ttcctctttc agaggttatt tcaggccatg
     6241 gtgctgcgc
//
