LOCUS       sequence-42591-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GRM3 (3SM9)
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     84..146
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             198..218
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tacagttttg tataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg
       61 ggcgcggatc tcggtccgaa accatgaaat tcttagtcaa cgttgccctt gtttttatgg
      121 tcgtatacat ttcttacatc tatgcggccg ctccggaaca tcaccatcac catcacgatt
      181 acgatatccc aacgaccgaa aacctgtatt ttcagggcgc catggatcat aactttctaa
      241 ggagagagat taaaatagaa ggtgaccttg ttttaggggg cctgtttcct attaacgaaa
      301 aaggcactgg aactgaagaa tgtgggcgaa tcaatgaaga ccgagggatt caacgcctgg
      361 aagccatgtt gtttgctatt gatgaaatca acaaagatga ttacttgcta ccaggagtga
      421 agttgggtgt tcacattttg gatacatgtt caagggatac ctatgcattg gagcaatcac
      481 tggagtttgt cagggcatct ttgacaaaag tggatgaagc tgagtatatg tgtcctgatg
      541 gatcctatgc cattcaagaa aacatcccac ttctcattgc aggggtcatt ggtggctctt
      601 atagcagtgt ttccatacag gtggcaaacc tgctgcggct cttccagatc cctcagatca
      661 gctacgcatc caccagcgcc aaactcagtg ataagtcgcg ctatgattac tttgccagga
      721 ccgtgccccc cgacttctac caggccaaag ccatggctga gatcttgcgc ttcttcaact
      781 ggacctacgt gtccacagta gcctccgagg gtgat
//
