LOCUS       sequence-425914-       10554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human NUP214. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_NUP214:1-2090
FEATURES             Location/Qualifiers
     source          1..10554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1737..1746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4395..4406
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    7617..7738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7628..7647)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(7853..7886)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        7896..7943
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7975..8769
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8029..8048)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8639..8658
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(8927..9515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9007..9026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    9641..9689
                     /note="synthetic polyadenylation signal"
     CDS             complement(9713..10111)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      10108..10117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10142..10499)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10156..10291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        10308..10499
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     10456..10476
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tgggagacga
     1261 gatggatgcc atgattcccg agcgggagat gaaggatttt cagtttagag cgctaaagaa
     1321 ggtgagaatc tttgactccc ctgaggaatt gcccaaggaa cgctcgagtc tgcttgctgt
     1381 gtccaacaaa tatggtctgg tcttcgctgg tggagccagt ggcttgcaga tttttcctac
     1441 taaaaatctt cttattcaaa ataaacccgg agatgatccc aacaaaatag ttgataaagt
     1501 ccaaggcttg ctagttccta tgaaattccc aatccatcac ctggccttga gctgtgataa
     1561 cctcacactc tctgcgtgca tgatgtccag tgaatatggt tccattattg ctttttttga
     1621 tgttcgcaca ttctcaaatg aggctaaaca gcaaaaacgc ccatttgcct atcataagct
     1681 tttgaaagat gcaggaggca tggtgattga tatgaagtgg aaccccactg tcccctccat
     1741 ggtggcagtt tgtctggctg atggtagtat tgctgtcctg caagtcacgg aaacagtgaa
     1801 agtatgtgca actcttcctt ccacggtagc agtaacctct gtgtgctgga gccccaaagg
     1861 aaagcagctg gcagtgggaa aacagaatgg aactgtggtc cagtatcttc ctactttgca
     1921 ggaaaaaaaa gtcattcctt gtcctccgtt ttatgagtca gatcatcctg tcagagttct
     1981 ggatgtgctg tggattggta cctacgtctt cgccatagtg tatgctgctg cagatgggac
     2041 cctggaaacg tctccagatg tggtgatggc tctactaccg aaaaaagaag aaaagcaccc
     2101 agagatattt gtgaacttta tggagccctg ttatggcagc tgcacggaga gacagcatca
     2161 ttactacctc agttacattg aggaatggga tttagtgctg gcagcatctg cggcttcaac
     2221 agaagttagt atccttgctc gacaaagtga tcagattaat tgggaatctt ggctactgga
     2281 ggattctagt cgagctgaat tgcctgtgac agacaagagt gatgactcct tgcccatggg
     2341 agttgtcgta gactatacaa accaagtgga aatcaccatc agtgatgaaa agactcttcc
     2401 tcctgctcca gttctcatgt tactttcaac agatggtgtg ctttgtccat tttatatgat
     2461 taatcaaaat cctggggtta agtctctcat caaaacacca gagcgacttt cattagaagg
     2521 agagcgacag cccaagtcac caggaagtac tcccactacc ccaacctcct ctcaagcccc
     2581 acagaaactg gatgcttctg cagctgcagc ccctgcctct ctgccacctt catcacctgc
     2641 tgctcccatt gccacttttt ctttgcttcc tgctggtgga gcccccactg tgttctcctt
     2701 tggttcttca tctttgaagt catcggctac ggtcactggg gagccccctt catattccag
     2761 tggctccgac agctccaaag cagccccagg ccctggccca tcaaccttct cttttgttcc
     2821 cccttctaaa gcctccctag cccccacccc tgcagcgtct cctgtggctc catcagctgc
     2881 ttcattctcc tttggatcat ctggttttaa gcctaccctg gaaagcacac cagtgccaag
     2941 tgtgtctgct ccaaatatag caatgaagtc ctccttccca ccctcaacct ctgctgtcaa
     3001 agtcaacctt agtgaaaagt ttactgctgc agctacctct actcctgtta gtagctccca
     3061 gagcgcaccc ccgatgtcgc cattctcttc tgcctccaag ccagctgctt ctggaccact
     3121 cagccacccc acacctctct cagcaccacc tagttccgtg ccattgaagt cctcagtctt
     3181 gccctcacca tcaggacgat ctgctcaggg cagttcaagc ccagtgccct caatggtaca
     3241 gaaatcaccc aggataaccc ctccagcggc aaagccaggc tctccccagg caaagtcact
     3301 tcagcctgct gttgcagaaa agcagggaca tcagtggaaa gattcagatc ctgtaatggc
     3361 tggaattggg gaggagattg cacactttca gaaggagttg gaagagttaa aagcccgaac
     3421 ttccaaagcc tgtttccaag tgggcacttc tgaggagatg aagatgctgc gaacagaatc
     3481 agatgacttg catacctttc ttttggagat taaagagacc acagagtcgc ttcatggaga
     3541 tataagtagc ctgaaaacaa ctttacttga gggctttgct ggtgttgagg aagccagaga
     3601 acaaaatgaa agaaatcgtg actctggtta tctgcatttg ctttataaaa gaccactgga
     3661 tcccaagagt gaagctcagc ttcaggaaat tcggcgcctt catcagtatg tgaaatttgc
     3721 tgtccaagat gtgaatgatg ttctagactt ggagtgggat cagcatctgg aacaaaagaa
     3781 aaaacaaagg cacctgcttg tgccagagcg agagacactg tttaacaccc tagccaacaa
     3841 tcgggaaatc atcaaccaac agaggaagag gctgaatcac ctggtggata gtcttcagca
     3901 gctccgcctt tacaaacaga cttccctgtg gagcctgtcc tcggctgttc cttcccagag
     3961 cagcattcac agttttgaca gtgacctgga aagcctgtgc aatgctttgt tgaaaaccac
     4021 catagaatct cacaccaaat ccttgcccaa agtaccagcc aaactgtccc ccatgaaaca
     4081 ggcacaactg agaaacttct tggccaagag gaagacccca ccagtgagat ccactgctcc
     4141 agccagcctg tctcgatcag cctttctgtc tcagagatat tatgaagact tggatgaagt
     4201 cagctcaacg tcatctgtct cccagtctct ggagagtgaa gatgcacgga cgtcctgtaa
     4261 agatgacgag gcagtggttc aggcccctcg gcacgccccc gtggttcgca ctccttccat
     4321 ccagcccagt ctcttgcccc atgcagcacc ttttgctaaa tctcacctgg ttcatggttc
     4381 ttcacctggt gtgatgggaa cttcagtggc tacatctgct agcaaaatta ttcctcaagg
     4441 ggccgatagc acaatgcttg ccacgaaaac cgtgaaacat ggtgcaccta gtccttccca
     4501 ccccatctca gccccgcagg cagctgccgc agcagcactc aggcggcaga tggccagtca
     4561 ggcaccagct gtaaacactt tgactgaatc aacgttgaag aatgtccctc aagtggtaaa
     4621 tgtgcaggaa ttgaagaata accctgcaac cccttctaca gccatgggtt cttcagtgcc
     4681 ctactccaca gccaaaacac ctcacccagt gttgacccca gtggctgcta accaagccaa
     4741 gcaggggtct ctaataaatt cccttaagcc atctgggcct acaccagcat ccggtcagtt
     4801 atcatctggt gacaaagctt cagggacagc caagatagaa acagctgtga cttcaacccc
     4861 atctgcttct gggcagttca gcaagccttt ctcattttct ccatcaggga ctggctttaa
     4921 ttttgggata atcacaccaa caccgtcttc taatttcact gctgcacaag gggcaacacc
     4981 ctccactaaa gagtcaagcc agccggacgc attctcatct ggtgggggaa gcaaaccttc
     5041 ttatgaggcc attcctgaaa gctcacctcc ctcaggaatc acatccgcat caaacaccac
     5101 cccaggagaa cctgccgcat ctagcagcag acctgtggca ccttctggaa ctgctctttc
     5161 caccacctct agtaagctgg aaaccccacc gtccaagctg ggagagcttc tgtttccaag
     5221 ttctttggct ggagagactc tgggaagttt ttcaggactg cgggttggcc aagcagatga
     5281 ttctacaaaa ccaaccaata aggcttcatc cacaagccta actagtaccc agccaaccaa
     5341 gacgtcaggc gtgccctcag ggtttaattt tactgccccc ccggtgttag ggaagcacac
     5401 ggagccccct gtgacatcct ctgcaaccac cacctcagta gcaccaccag cagccaccag
     5461 cacttcctca actgccgttt ttggcagtct gccagtcacc agtgcaggat cctctggggt
     5521 catcagtttt ggtgggacat ctctaagtgc tggcaagact agtttttcat ttggaagcca
     5581 acagaccaat agcacagtgc ccccatctgc cccaccacca actacagctg ccactcccct
     5641 tccaacatca ttccccacat tgtcatttgg tagcctcctg agttcagcaa ctaccccctc
     5701 cctgcctatg tccgctggca gaagcacaga agaggccact tcatcagctt tgcctgagaa
     5761 gccaggtgac agtgaggtct cagcatcagc agcctcactt ctagaggagc aacagtcagc
     5821 ccagcttccc caggctcctc cgcaaacttc tgactctgtt aaaaaagaac ctgttcttgc
     5881 ccagcctgca gtcagcaact ctggcactgc agcatctagt actagtcttg tagcactttc
     5941 tgcagaggct accccagcca ccacgggggt ccctgatgcc aggacggagg cagtaccacc
     6001 tgcttcctcc ttttctgtgc ctgggcagac tgctgtcaca gcagctgcta tctcaagtgc
     6061 aggccctgtg gccgtcgaaa catcaagtac ccccatagcc tccagcacca cgtccattgt
     6121 tgctcccggc ccatctgcag aggcagcagc atttggtacc gtcacttctg gctcatccgt
     6181 ctttgctcag cctcctgctg ccagttctag ctcagctttc aaccagctca ccaacaacac
     6241 agccactgcc ccctctgcca cgcccgtgtt tgggcaagtg gcagccagca ccgcaccaag
     6301 tctgtttggg cagcagactg gtagcacagc cagcacagca gctgccacac cacaggtcag
     6361 cagctcaggg tttagcagcc cagcttttgg taccacagcc ccaggggtct ttggacagac
     6421 aaccttcggg caggcctcag tctttgggca gtcggcgagc agtgctgcaa gtgtcttttc
     6481 cttcagtcag cctgggttca gttccgtgcc tgccttcggt cagcctgctt cctccactcc
     6541 cacatccacc agtggaagtg tctttggtgc cgcctcaagt accagtagct ccagttcctt
     6601 ctcatttgga cagtcttctc ccaacacagg aggggggctg tttggccaaa gcaacgctcc
     6661 tgcttttggg cagagtcctg gctttggaca gggaggctct gtctttggtg gtacctcagc
     6721 tgccaccaca acagcagcaa cctctgggtt cagcttttgc caagcttcag gttttgggtc
     6781 tagtaatact ggttctgtgt ttggtcaagc agccagtact ggtggaatag tctttggcca
     6841 gcaatcatcc tcttccagtg gtaacgtgtt tgggtctgga aacactggaa gagggggagg
     6901 tttcttcagt ggccttggag gaaaacccag tcaggatgca gccaacaaaa acccattcag
     6961 ctcggccagt gggggctttg gatccacagc tacctcaaat acctctaacc tatttggaaa
     7021 cagtggggcc aagacatttg gtggatttgc cagctcgtcg tttggagagc agaaacccac
     7081 tggcactttc agctctggag gaggaagtgt ggcatcccaa ggctttgggt tttcctctcc
     7141 aaacaaaaca ggtggcttcg gtgctgctcc agtgtttggc agccctccta cttttggggg
     7201 atcccctggg tttggagggg tgccagcatt cggttcagcc ccagccttta caagccctct
     7261 gggctcgacg ggaggcaaag tgttcggaga gggcactgca gctgccagcg caggaggatt
     7321 cgggtttggg agcagcagca acaccacatc cttcggcacg ctcgcgagtc agaatgcccc
     7381 cactttcgga tcactgtccc aacagacttc tggttttggg acccagagta gcggattctc
     7441 tggttttgga tcaggcacag gagggttcag ctttgggtca aataactcgt ctgtccaggg
     7501 ttttggtggc tggcgaagct aatctagagg tcaagcttcg ggggtttaaa cctgcaggca
     7561 tgcaagctga tttggctgct gccaccgctg agcaaggccg cttcgagcag acatgataag
     7621 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     7681 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     7741 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggttttttt
     7801 aagcaagtaa aacctctaca aatgtggtaa aatcttaatt aagacttcta atataacttc
     7861 gtatagcata cattatacga agttatggtt ccactgttga caattaatca tcggcatagt
     7921 atatcggcat agtataatac gacaaggtga ggaactaaac ccaggaggca gatcatgatt
     7981 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     8041 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     8101 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     8161 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     8221 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     8281 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     8341 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8401 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8461 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8521 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8581 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8641 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8701 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8761 ttcttctgat tcgaaatgac cgaccaagcg acgcccaacc ggtatcagct cactcaaagg
     8821 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9361 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9481 agcagcagat tacgcgcaga aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg
     9541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9601 aaaggatctt cacctagatc cttttatagt ccggaatcgt cacacaaaaa accaacacac
     9661 agatgtaatg aaaataaaga tattttattg cggccatcgt gatggctaga gtttagccct
     9721 cccacacata accagagggc agcaattcac gaatcccaac tgccgtcggc tgtccatcac
     9781 tgtccttcac tatggctttg atcccaggat gcagatcgag aagcacctgt cggcaccgtc
     9841 cgcaggggct caagatgccc ctgttctcat ttccgatcgc gacgatacaa gtcaggttgc
     9901 cagctgccgc agcagcagca gtgcccagca ccacgagttc tgcacaaggt cccccagtaa
     9961 aatgatatac attgacacca gtgaagatgc ggccgtcgct agagagagct gcgctggcga
    10021 cgctgtagtc ttcagagatg gggatgctgt tgattgtagc cgttgctctt tcaatgaggg
    10081 tggattcttc ttgagacaaa ggcttggcca tggtggcgct agtgtcagaa gaatcgagct
    10141 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
    10201 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
    10261 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
    10321 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
    10381 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
    10441 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcaga
    10501 aggtacctaa ccaagttcct ctttcagagg ttatttcagg ccatggtgct gcgc
//
