LOCUS       sequence-42592-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CYP7A1 (3SN5)
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(149..154,155..172,173..179)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    187..203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(258..263,264..279,280..286)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    294..310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(353..358,359..374,375..381)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    389..405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcaccat tcgatggtgt
       61 cctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag gatcgatcca tcgatgagct
      241 tactccccat ccccctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
      301 cggataacaa tttcncacag gaaacangat cagcttactc cccatccccc tgttgacaat
      361 taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      421 aggatccatc gatgcttagg aggtcatatg gctaagaaaa cgagctctag gagaaggcaa
      481 acgggtgaac cacctctaga gaatggatta attccatacc tgggctgtgc tctgcaattt
      541 ggtgccaatc ctcttgagtt cctcagagca aatcaaagga aacatggtca tgtttttacc
      601 tgcaaactaa tgggaaaata tgtccatttc atcacaaatc ccttgtcata ccataaggtg
      661 ttgtgccacg gaaaatattt tgattggaaa aaatttcact ttgctctttc tgcgaaggca
      721 tttgggcaca gaagcattga cccgatggat ggaaatacca ctgaaaacat aaacgacact
      781 ttcatcaaaa ccctgcaggg ccatgccttg aattccctca cggaaagcat gatggaaaac
      841 ctccaacgta tcatgagacc tcc
//
