LOCUS       sequence-425924-        6477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human STRN isoform 2. N-terminal
            HiBiT tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_STRN:1-731
FEATURES             Location/Qualifiers
     source          1..6477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3540..3661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3551..3570)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3776..3809)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3819..3866
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3898..4692
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3952..3971)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4562..4581
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4850..5438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4930..4949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5564..5612
                     /note="synthetic polyadenylation signal"
     CDS             complement(5636..6034)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6031..6040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6065..6422)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6079..6214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6231..6422
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6379..6399
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggacgagca
     1261 ggcgggtccc ggcgtcttct tcagcaacaa ccacccgggc gccggcggtg ccaaggggct
     1321 cgggcctctg gcgggggcgg cccgagccca gtacagtctc ccggggatcc tgcacttcct
     1381 gcagcacgag tgggcccgct tcgaggtgga gagagcccag tgggaggtgg agcgggcgga
     1441 gctgcaggcc cagattgcct tcctgcaggg agaaaggaag ggccaagaaa atttgaagaa
     1501 ggatcttgtg aggaggatca aaatgttgga gtatgctctt aaacaggaaa gagccaaata
     1561 ccacaagttg aaatacggga cagaattgaa tcagggagat atgaagcctc caagctatga
     1621 ttctgatgaa ggtaatgaaa cagaagtgca gccacaacaa aacagccagt taatgtggaa
     1681 acaaggtcga caactactca gacagtatct acaggaggtg ggttatacag atactattct
     1741 agatgtgaaa tctaaacgag tgcgagcttt gttgggcttt tcaagtgatg tcacggacag
     1801 ggaagatgac aaaaatcagg actcagttgt aaatggcaca gaggctgaag ttaaagagac
     1861 agcaatgatt gcaaaatctg agttaacaga ttctgcctcc gtgctggata atttcaaatt
     1921 ccttgaaagt gcagctgcag atttcagtga tgaagatgaa gatgatgatg ttgatggaag
     1981 agagaaaagc gtcattgata cttcaacaat tgttaggaaa aaagcattgc ctgacagcgg
     2041 tgaagatcga gatacaaaag aagctctaaa ggagtttgac ttcttggtta catcagagga
     2101 aggagacaat gaatctagaa gtgcaggcga tggaacagac tgggggccca ataggtcaaa
     2161 actacaagat atgcttgcta atttgagaga tgttgatgaa cttccttcat tgcagccatc
     2221 tgtgggttca ccttccagac ccagcagctc caggcttcct gaacatgaaa ttaatagggc
     2281 agatgaagtg gaagcattga catttcctcc ttcttctgga aagtcattca tcatgggagc
     2341 agatgaagcc cttgaaagtg aactgggact tggagaacta gcaggcctta cggtggccaa
     2401 tgaagcagac tcactaactt atgatatagc aaacaataaa gatgcattga ggaagacatg
     2461 gaaccctaag tttacattga gaagtcactt tgatggcatc cgagcccttg ctttccatcc
     2521 cattgagcct gttttgataa cagcatcaga ggatcacaca ttaaaaatgt ggaatttaca
     2581 gaaaacagcc ccagccaaaa agagcacttc tcttgatgta gaacctatct atacattcag
     2641 agcccataaa ggtccagtgc tttgtgtggt aatgagcagc aatggtgagc agtgttacag
     2701 tggtggtact gatggactga tccagggctg gaataccact aatcccaaca tcgaccccta
     2761 tgattcttat gatccttctg ttttacgagg ccctctgcta ggccacacgg atgcagtctg
     2821 gggtttggct tatagtgcag cacatcagcg tttgttgtcc tgttcagcag atggcactct
     2881 gcgtttatgg aatacaactg aggttgctcc agcactaagt gtatttaatg atactaaaga
     2941 actgggaatc cctgcctctg tggatctagt gagcagtgac ccgagccata tggtagcatc
     3001 attcagcaag ggatatacaa gcatttttaa catggaaaca caacaacgca ttctcacttt
     3061 agaatccaat gtagatacaa cagccaactc ttcctgccaa ataaatagag tcatcagtca
     3121 tcctactctt ccgatcagca tcactgctca tgaagacagg cacatcaaat tctatgataa
     3181 caatacaggc aaactgatcc actcgatggt agcccaccta gaagctgtta caagtttagc
     3241 agttgatccc aatggccttt acttgatgtc tggcagtcat gactgttcaa tacgtttatg
     3301 gaatctagaa agtaagacgt gtatccaaga attcacagct catcgaaaaa agtttgaaga
     3361 atcgattcat gatgtagctt tccacccatc caaatgctat atagccagtg ctggagctga
     3421 cgcactggct aaagtctttg tataatctag aggtcaagct tcgggggttt aaacctgcag
     3481 gcatgcaagc tgatttggct gctgccaccg ctgagcaagg ccgcttcgag cagacatgat
     3541 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     3601 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     3661 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     3721 tttaagcaag taaaacctct acaaatgtgg taaaatctta attaagactt ctaatataac
     3781 ttcgtatagc atacattata cgaagttatg gttccactgt tgacaattaa tcatcggcat
     3841 agtatatcgg catagtataa tacgacaagg tgaggaacta aacccaggag gcagatcatg
     3901 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     3961 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4021 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     4081 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4141 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4201 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4261 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4321 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4381 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     4441 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4501 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     4561 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     4621 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     4681 gagttcttct gattcgaaat gaccgaccaa gcgacgccca accggtatca gctcactcaa
     4741 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4801 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4861 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4921 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4981 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5041 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5101 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5161 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5221 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5281 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5341 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5401 gcaagcagca gattacgcgc agaaaaaaag gatttcaaga agatcctttg atcttttcta
     5461 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5521 caaaaaggat cttcacctag atccttttat agtccggaat cgtcacacaa aaaaccaaca
     5581 cacagatgta atgaaaataa agatatttta ttgcggccat cgtgatggct agagtttagc
     5641 cctcccacac ataaccagag ggcagcaatt cacgaatccc aactgccgtc ggctgtccat
     5701 cactgtcctt cactatggct ttgatcccag gatgcagatc gagaagcacc tgtcggcacc
     5761 gtccgcaggg gctcaagatg cccctgttct catttccgat cgcgacgata caagtcaggt
     5821 tgccagctgc cgcagcagca gcagtgccca gcaccacgag ttctgcacaa ggtcccccag
     5881 taaaatgata tacattgaca ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg
     5941 cgacgctgta gtcttcagag atggggatgc tgttgattgt agccgttgct ctttcaatga
     6001 gggtggattc ttcttgagac aaaggcttgg ccatggtggc gctagtgtca gaagaatcga
     6061 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     6121 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     6181 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     6241 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     6301 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     6361 agcctgggga ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc
     6421 agaaggtacc taaccaagtt cctctttcag aggttatttc aggccatggt gctgcgc
//
