LOCUS       sequence-425925-        6684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human TAF5. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_TAF5:1-800
FEATURES             Location/Qualifiers
     source          1..6684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3747..3868
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3758..3777)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3983..4016)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4026..4073
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4105..4899
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4159..4178)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4769..4788
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(5057..5645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5137..5156)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5771..5819
                     /note="synthetic polyadenylation signal"
     CDS             complement(5843..6241)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      6238..6247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6272..6629)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6286..6421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6438..6629
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6586..6606
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggcggcgct
     1261 ggcggaggag cagacggagg tggcggtcaa gctagagcct gagggaccgc caacgctgct
     1321 acctccgcag gcgggggacg gcgcaggcga gggtagcggc ggcactacca acaacggccc
     1381 caacggcggc ggcgggaacg ttgcggcgtc gtcgtccact ggcggggatg gcgggacccc
     1441 caagcccacg gtggctgtct ccgccgctgc cccggcgggg gcggccccgg tgcccgccgc
     1501 tgctccggac gccggcgctc cgcatgaccg acagactcta ctggccgtgc tgcagttcct
     1561 acggcagagc aaactccgcg aggccgaaga ggcgctgcgc cgtgaggccg ggctgctgga
     1621 ggaggcagtg gcgggctccg gagccccggg agaggtggac agcgccggcg ctgaggtgac
     1681 cagcgcgctt ctcagccggg tgaccgcctc ggcccctggc cctgcggccc ccgaccctcc
     1741 gggcactggc gcttcggggg ccacggtcgt ctcaggttca gcctcaggtc ctgcggctcc
     1801 gggtaaagtt ggaagtgttg ctgtggaaga ccagccagat gtcagtgccg tgttgtcagc
     1861 ctacaaccaa caaggagatc ccacaatgta tgaagaatac tatagtggac tgaaacactt
     1921 cattgaatgt tccctggact gccatcgggc agagttgtcc caactttttt atcctctgtt
     1981 tgtgcacatg tacttggagc tagtctacaa tcaacatgag aatgaagcaa agtcattctt
     2041 tgagaagttc catggagatc aggaatgtta ttaccaggat gacctacgag tattatctag
     2101 tcttaccaaa aaggaacaca tgaaagggaa tgagaccatg ttggattttc gaacaagtaa
     2161 atttgttctg cgtatttccc gtgactcgta ccaactcttg aagaggcatc ttcaggagaa
     2221 acagaacaat cagatatgga acatagttca ggagcacctc tacattgaca tctttgatgg
     2281 gatgccgcgt agtaagcaac agatagatgc gatggtggga agtttggcag gagaggctaa
     2341 acgagaggca aacaaatcaa aggtattttt tggtttatta aaagaaccag aaattgaggt
     2401 acctttggat gacgaggatg aagagggaga aaatgaagaa ggaaaaccta aaaagaagaa
     2461 gcctaaaaaa gatagtattg gatccaaaag caaaaaacaa gatcccaatg ctccacctca
     2521 gaacagaatc cctcttcctg agttgaaaga ttcagataag ttggataaga taatgaatat
     2581 gaaagaaacc accaaacgag tgcgccttgg gccggactgc ttaccctcca tttgtttcta
     2641 tacatttctc aatgcttacc agggtctcac tgcagtggat gtcactgatg attctagtct
     2701 gattgctgga ggttttgcag attcaactgt cagagtgtgg tcggtaacac ccaaaaagct
     2761 tcgtagtgtc aaacaagcat cagatcttag tcttatagac aaagaatcag atgatgtctt
     2821 agaaagaatc atggatgaga aaacagcaag tgagttgaag attttgtatg gtcacagtgg
     2881 gcctgtctac ggagccagct tcagtccgga taggaactat ctgctttcct cttcagagga
     2941 cggaactgtt agattgtgga gccttcaaac atttacttgt ttggtgggat ataaaggaca
     3001 caactatcca gtatgggaca cacaattttc tccatatgga tattattttg tgtcaggggg
     3061 ccatgaccga gtagctcggc tctgggctac agaccactat cagcctttaa gaatatttgc
     3121 cggccatctt gctgatgtga attgtaccag attccatcca aattctaatt atgttgctac
     3181 gggctctgca gacagaactg tgcggctctg ggacgtcctg aatggtaact gtgtaaggat
     3241 cttcactgga cacaagggac caattcattc cttgacattt tctcccaatg ggagattcct
     3301 ggctacagga gcaacagatg gcagagtgct tctttgggat attggacatg gtttgatggt
     3361 tggagaatta aaaggccaca ctgatacagt ctgttcactt aggtttagta gagatggtga
     3421 aattttggca tcaggttcaa tggataatac agttcgatta tgggatgcta tcaaagcctt
     3481 tgaagattta gagaccgatg actttactac agccactggg catataaatt tacctgagaa
     3541 ttcacaggag ttattgttgg gaacatatat gaccaaatca acaccagttg tacaccttca
     3601 ttttactcga agaaacctgg ttctagctgc aggagcttat agtccacaat aatctagagg
     3661 tcaagcttcg ggggtttaaa cctgcaggca tgcaagctga tttggctgct gccaccgctg
     3721 agcaaggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     3781 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3841 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3901 ggttcagggg gagatgtggg aggttttttt aagcaagtaa aacctctaca aatgtggtaa
     3961 aatcttaatt aagacttcta atataacttc gtatagcata cattatacga agttatggtt
     4021 ccactgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4081 ggaactaaac ccaggaggca gatcatgatt gaacaagatg gattgcacgc aggttctccg
     4141 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4201 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4261 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     4321 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4381 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4441 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4501 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4561 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4621 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4681 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4741 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4801 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4861 cgcatcgcct tctatcgcct tcttgacgag ttcttctgat tcgaaatgac cgaccaagcg
     4921 acgcccaacc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4981 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5041 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5101 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5161 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5221 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5281 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5341 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5401 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5461 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5521 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5581 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5641 ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5701 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt
     5761 ccggaatcgt cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg
     5821 cggccatcgt gatggctaga gtttagccct cccacacata accagagggc agcaattcac
     5881 gaatcccaac tgccgtcggc tgtccatcac tgtccttcac tatggctttg atcccaggat
     5941 gcagatcgag aagcacctgt cggcaccgtc cgcaggggct caagatgccc ctgttctcat
     6001 ttccgatcgc gacgatacaa gtcaggttgc cagctgccgc agcagcagca gtgcccagca
     6061 ccacgagttc tgcacaaggt cccccagtaa aatgatatac attgacacca gtgaagatgc
     6121 ggccgtcgct agagagagct gcgctggcga cgctgtagtc ttcagagatg gggatgctgt
     6181 tgattgtagc cgttgctctt tcaatgaggg tggattcttc ttgagacaaa ggcttggcca
     6241 tggtggcgct agtgtcagaa gaatcgagct ttttgcaaaa gcctaggcct ccaaaaaagc
     6301 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     6361 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     6421 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     6481 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
     6541 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     6601 cattccacag ctggttcttt ccgcctcaga aggtacctaa ccaagttcct ctttcagagg
     6661 ttatttcagg ccatggtgct gcgc
//
