LOCUS       sequence-425928-        6105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human ATG16L1. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_ATG16L1:1-607
FEATURES             Location/Qualifiers
     source          1..6105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3168..3289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3179..3198)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3404..3437)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3447..3494
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3526..4320
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3580..3599)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4190..4209
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4478..5066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4558..4577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5192..5240
                     /note="synthetic polyadenylation signal"
     CDS             complement(5264..5662)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5659..5668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5693..6050)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5707..5842
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5859..6050
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6007..6027
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tgtcgtcggg
     1261 cctccgcgcc gctgacttcc cccgctggaa gcgccacatc tcggagcaac tgaggcgccg
     1321 ggaccggctg cagagacagg cgttcgagga gatcatcctg cagtataaca aattgctgga
     1381 aaagtcagat cttcattcag tgttggccca gaaactacag gctgaaaagc atgacgtacc
     1441 aaacaggcac gagataagtc ccggacatga tggcacatgg aatgacaatc agctacaaga
     1501 aatggcccaa ctgaggatta agcaccaaga ggaactgact gaattacaca agaaacgtgg
     1561 ggagttagct caactggtga ttgacctgaa taaccaaatg cagcggaagg acagggagat
     1621 gcagatgaat gaagcaaaaa ttgcagaatg tttgcagact atctctgacc tggagacgga
     1681 gtgcctagac ctgcgcacta agctttgtga ccttgaaaga gccaaccaga ccctgaagga
     1741 tgaatatgat gccctgcaga tcacttttac tgccttggag ggaaaactga ggaaaactac
     1801 ggaagagaac caggagctgg tcaccagatg gatggctgag aaagcccagg aagccaatcg
     1861 gcttaatgca gagaatgaaa aagactccag gaggcggcaa gcccggctgc agaaagagct
     1921 tgcagaagca gcaaaggaac ctctaccagt cgaacaggat gatgacattg aggtcattgt
     1981 ggatgaaact tctgatcaca cagaagagac ctctcctgtg cgagccatca gcagagcagc
     2041 cactaagcga ctctcgcagc ctgctggagg ccttctggat tctatcacta atatctttgg
     2101 gagacgctct gtctcttcct tcccagtccc ccaggacaat gtggatgctc atcctggttc
     2161 tggtaaagaa gtgagggtac cagctactgc cttgtgtgtc ttcgatgcac atgatgggga
     2221 agtcaacgct gtgcagttca gtccaggttc ccggttactg gccactggag gcatggaccg
     2281 cagggttaag ctttgggaag tatttggaga aaaatgtgag ttcaagggtt ccctatctgg
     2341 cagtaatgca ggaattacaa gcattgaatt tgatagtgct ggatcttacc tcttagcagc
     2401 ttcaaatgat tttgcaagcc gaatctggac tgtggatgat tatcgattac ggcacacact
     2461 cacgggacac agtgggaaag tgctgtctgc taagttcctg ctggacaatg cgcggattgt
     2521 ctcaggaagt cacgaccgga ctctcaaact ctgggatcta cgcagcaaag tctgcataaa
     2581 gacagtgttt gcaggatcca gttgcaatga tattgtctgc acagagcaat gtgtaatgag
     2641 tggacatttt gacaagaaaa ttcgtttctg ggacattcga tcagagagca tagttcgaga
     2701 gatggagctg ttgggaaaga ttactgccct ggacttaaac ccagaaagga ctgagctcct
     2761 gagctgctcc cgtgatgact tgctaaaagt tattgatctc cgaacaaatg ctatcaagca
     2821 gacattcagt gcacctgggt tcaagtgcgg ctctgactgg accagagttg tcttcagccc
     2881 tgatggcagt tacgtggcgg caggctctgc tgagggctct ctgtatatct ggagtgtgct
     2941 cacagggaaa gtggaaaagg ttctttcaaa gcagcacagc tcatccatca atgcggtggc
     3001 gtggtcgccc tctggctcgc acgttgtcag tgtggacaaa ggatgcaaag ctgtgctgtg
     3061 ggcacagtac taatctagag gtcaagcttc gggggtttaa acctgcaggc atgcaagctg
     3121 atttggctgc tgccaccgct gagcaaggcc gcttcgagca gacatgataa gatacattga
     3181 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3241 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3301 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta
     3361 aaacctctac aaatgtggta aaatcttaat taagacttct aatataactt cgtatagcat
     3421 acattatacg aagttatggt tccactgttg acaattaatc atcggcatag tatatcggca
     3481 tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat tgaacaagat
     3541 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3601 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3661 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     3721 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3781 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3841 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3901 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3961 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4021 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4081 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4141 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4201 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4261 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4321 ttcgaaatga ccgaccaagc gacgcccaac cggtatcagc tcactcaaag gcggtaatac
     4381 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4441 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4501 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4561 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4621 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4681 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4741 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4801 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4861 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     4921 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4981 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5041 ttacgcgcag aaaaaaagga tttcaagaag atcctttgat cttttctacg gggtctgacg
     5101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5161 tcacctagat ccttttatag tccggaatcg tcacacaaaa aaccaacaca cagatgtaat
     5221 gaaaataaag atattttatt gcggccatcg tgatggctag agtttagccc tcccacacat
     5281 aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca
     5341 ctatggcttt gatcccagga tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc
     5401 tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg ccagctgccg
     5461 cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta aaatgatata
     5521 cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg acgctgtagt
     5581 cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg gtggattctt
     5641 cttgagacaa aggcttggcc atggtggcgc tagtgtcaga agaatcgagc tttttgcaaa
     5701 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     5761 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     5821 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     5881 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     5941 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     6001 ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag aaggtaccta
     6061 accaagttcc tctttcagag gttatttcag gccatggtgc tgcgc
//
