LOCUS       sequence-425929-        6144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full-length human CFAP52. N-terminal HiBiT 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pBiT3.1-N_CFAP52:1-620
FEATURES             Location/Qualifiers
     source          1..6144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    24..105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        338..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     796..816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          981..1113
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1096..1115
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1157..1176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1157..1175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3207..3328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3218..3237)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3443..3476)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3486..3533
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3565..4359
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3619..3638)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4229..4248
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4517..5105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4597..4616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5231..5279
                     /note="synthetic polyadenylation signal"
     CDS             complement(5303..5701)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      5698..5707
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5732..6089)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5746..5881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5898..6089
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6046..6066
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat
       61 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
      121 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
      181 ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc
      241 tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
      781 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctggttt agtgaaccgt cagatcacta gaatctttat tgcggtagtt tatcacagtt
      901 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt
      961 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
     1021 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
     1081 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
     1141 cttaaggcta gagtattaat acgactcact ataggggtta gcgatcgcca tggtgagcgg
     1201 ctggcggctg ttcaagaaga ttagcgggag ctccggtggc tcgagcggta tggataacaa
     1261 aatttcgccg gaggcccaag tggcggagct ggaacttgac gccgtgatcg gcttcaatgg
     1321 acatgtgccc actggtctca aatgccatcc tgaccaggag catatgattt atcctcttgg
     1381 ttgcacagtc ctcattcagg caataaatac taaagagcag aacttcctac agggtcatgg
     1441 caacaacgtc tcctgcttgg ccatctccag gtctggagag tacatcgcct ccggacaagt
     1501 cacattcatg gggttcaagg tagacatcat tttgtgggat tataagaaca gagagctgct
     1561 tgctcggctg tcccttcaca aaggcaaaat tgaagctctg gccttttctc caaatgattt
     1621 gtacttggta tcactaggag gcccagatga cggaagtgtg gtggtgtgga gcatagccaa
     1681 gagagatgcc atctgtggca gccctgcagc cggcctcaat gttggcaatg ccaccaatgt
     1741 gatcttctcc aggtgccggg atgagatgtt tatgactgct ggaaatggga caattcgagt
     1801 atgggaattg gatcttccaa atagaaaaat ctggccaact gagagccaaa caggacagtt
     1861 gaaaagaata gtcatgagta ttggagtgga tgatgatgat agctttttct accttggcac
     1921 cacgactgga gatattctaa aaatgaaccc caggactaaa ctgctgacag atgttgggcc
     1981 tgcgaaggac aaattcagtt tgggagtgtc agctatcagg tgcctgaaga tggggggttt
     2041 gttggtgggc tctggagccg gactgctggt cttctgtaaa agccctggct acaaacccat
     2101 caagaagatt cagttacaag gcggcatcac ttctatcaca cttcgaggag aaggacacca
     2161 gtttctcgta ggaacagaag aatcgcacat ttatcgtgtc agcttcacgg atttcaaaga
     2221 gacgctcata gcgacttgtc actttgatgc tgtcaaggat attgtctttc catttggcac
     2281 tgctgagcta tttgcaacct gtgccaagaa ggatatcagg gtgtggcaca catcatccaa
     2341 cagggagctg ctgcggatca ccgtgcccaa catgacctgc cacggcatcg acttcatgag
     2401 ggacggcaaa agcatcattt cagcatggaa cgacggtaaa atccgagcct tcgccccaga
     2461 gacaggccga ctgatgtatg tcattaacaa tgctcacagg atcggcgtca ccgccatcgc
     2521 caccaccagt gactgtaaaa gggtcatcag tggcggtggg gaaggggagg tgagggtatg
     2581 gcagataggc tgtcagaccc agaagctgga ggaggccctg aaggaacaca agtcatcagt
     2641 gtcctgcatt agggtgaaga ggaacaacga ggagtgtgtc accgccagca ccgatgggac
     2701 ttgtatcatt tgggaccttg tgcgtctcag gaggaatcag atgatactag ccaacacctt
     2761 attccagtgt gtgtgctatc accctgagga gttccagatc atcaccagcg gaacagacag
     2821 aaagattgct tactgggaag tatttgatgg gacagtaatc agagaattgg aaggttccct
     2881 gtctgggtcg ataaatggca tggatatcac acaggaaggg gtgcactttg tcacaggtgg
     2941 aaatgaccat ctggtcaaag tttgggatta taatgagggt gaagtgactc acgttggggt
     3001 gggacacagt ggcaacatca cacgcatccg cataagtcca ggaaatcaat atattgttag
     3061 tgtaagtgcc gatggagcca ttttgcgatg gaagtaccca tatacctcct aatctagagg
     3121 tcaagcttcg ggggtttaaa cctgcaggca tgcaagctga tttggctgct gccaccgctg
     3181 agcaaggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     3241 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3301 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3361 ggttcagggg gagatgtggg aggttttttt aagcaagtaa aacctctaca aatgtggtaa
     3421 aatcttaatt aagacttcta atataacttc gtatagcata cattatacga agttatggtt
     3481 ccactgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     3541 ggaactaaac ccaggaggca gatcatgatt gaacaagatg gattgcacgc aggttctccg
     3601 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3661 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3721 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     3781 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     3841 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     3901 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     3961 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4021 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4081 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4141 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4201 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4261 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4321 cgcatcgcct tctatcgcct tcttgacgag ttcttctgat tcgaaatgac cgaccaagcg
     4381 acgcccaacc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4921 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5041 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5101 ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5161 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttatagt
     5221 ccggaatcgt cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg
     5281 cggccatcgt gatggctaga gtttagccct cccacacata accagagggc agcaattcac
     5341 gaatcccaac tgccgtcggc tgtccatcac tgtccttcac tatggctttg atcccaggat
     5401 gcagatcgag aagcacctgt cggcaccgtc cgcaggggct caagatgccc ctgttctcat
     5461 ttccgatcgc gacgatacaa gtcaggttgc cagctgccgc agcagcagca gtgcccagca
     5521 ccacgagttc tgcacaaggt cccccagtaa aatgatatac attgacacca gtgaagatgc
     5581 ggccgtcgct agagagagct gcgctggcga cgctgtagtc ttcagagatg gggatgctgt
     5641 tgattgtagc cgttgctctt tcaatgaggg tggattcttc ttgagacaaa ggcttggcca
     5701 tggtggcgct agtgtcagaa gaatcgagct ttttgcaaaa gcctaggcct ccaaaaaagc
     5761 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     5821 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     5881 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     5941 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
     6001 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     6061 cattccacag ctggttcttt ccgcctcaga aggtacctaa ccaagttcct ctttcagagg
     6121 ttatttcagg ccatggtgct gcgc
//
